Antibacterial activities of amorphous cefuroxime axetil ultrafine particles prepared by high gravity antisolvent precipitation (HGAP)

Abstract

In vitro and in vivo antibacterial activities on the Staphylococcus aureus and Escherichia coli of the amorphous cefuroxime axetil (CFA) ultrafine particles prepared by HGAP method were investigated in this paper. The conventional sprayed CFA particles were studied as the control group. XRD, SEM, BET tests were performed to investigate the morphology changes of the samples before and after sterile. The in vitro dissolution test, minimal inhibitory concentrations (MIC) and the in vivo experiment on mice were explored. The results demonstrated that: (i) The structure, morphology and amorphous form of the particles could be affected during steam sterile process; (ii) CFA particles with different morphologies showed varied antibacterial activities; and (iii) the in vitro and in vivo antibacterial activities of the ultrafine particles prepared by HGAP is markedly stronger than that of the conventional sprayed amorphous particles.

Accelerated Aqueous Solubility and Antibacterial Activity of Cefuroxime Axetil Using Microcrystalline Cellulose as Carrier

“…Omolo et al (2018) demonstrated that antibacterial activities of different nanosuspension formulations of fusidic acid delivered through poloxamer 188 were found better as compared to pure fucidic acid [28]. Further, it was revealed that both in-vitro and in-vivo test of CA amorphous ultrafine particles by high gravity anti-solvent precipitation (HGAP) technique possessed better dissolution rate and stronger antibacterial activity by agar dilution method against S. aureus and E. coli [29].…”

Draft Genome Sequence of Clostridium butyricum Strain 16-3, Isolated from Neonatal Feces

“…In total, 202,356 PacBio subreads (average subread length, 4,223 bp; N 50 , 6,195 bp) were generated. PacBio reads were assembled using the RS Hierarchical Genome Assembly Process (HGAP) protocol v3.0 ( 9 – 11 ). Default parameters were used except where otherwise noted.…”

Complete Genome Sequence of Lacticaseibacillus rhamnosus CAU 1365, Isolated from Kimchi

“…The sequences were assembled de novo with RS Hierarchical Genome Assembly Process (HGAP) Assembly v. 3.0 using PacBio library preparation. De novo assembly consisted of assembly with PreAssembler v. 2.0, alignment by Basic Local Alignment with Successive Refinement (BLASR) v. 1.0, and assembly polishing with Quiver v. 1.0 in single-molecule real-time (SMRT) Portal v. 2.3 ( 5 – 7 ). The contig was identified as circular based on the presence of identical ends.…”