Antibacterial Activities and Antibacterial Mechanism of Polygonum cuspidatum Extracts against Nosocomial Drug-Resistant Pathogens

Abstract

Abstract:Recently, drug resistance due to the extensive abuse and over-use of antibiotics has become an increasingly serious problem, making the development of alternative antibiotics a very urgent issue. In this study, the Chinese herbal medicine, Polygonum cuspidatum, was extracted with 95% ethanol and the crude extracts were further purified by partition based on solvent polarity. The antimicrobial activities of the extracts and fractions were determined by the disk diffusion and minimum inhibitory concentration (MIC) methods. The results showed that the ethyl ether fraction (EE) of the ethanol extracts possesses a broader antimicrobial spectrum and greater antimicrobial activity against all of the tested clinical drug-resistant isolates, with a range of MIC values between 0.1-3.5 mg/mL. The active extract showed complete inhibition of pathogen growth and did not induce resistance to the active components. In addition, according to scanning electron microscope observations, EE resulted in greater cell morphological changes by degrading and disrupting the cell wall and cytoplasmic membrane, whereby ultimately this cell membrane integrity damage led to cell death. In conclusion, the EE extracts from Polygonum cuspidatum may provide a promising antimicrobial agent for therapeutic applications against nosocomial drug-resistant bacteria.

In-Vitro Antimicrobial Activity of Herbal Extracts From Tabuk Region (Kingdom of Saudi Arabia) Against Nosomial Pathogens: A Preliminary Study

“…A study conducted by Su, et al (2015) revealed that ethyl ether extraction from Polygonum cuspidatum tends to provide a promising and capable agent for therapeutic applications against nosocomial pathogens. The drug resistant gene transfer occurs due to the spread of drug resistant bacteria among different hosts.…”

In vitro Antibacterial, Antioxidant and Anti-Inflammatory Effects of Senecio asperulus and Gunnera perpensa from Mohale's Hoek, Lesotho

“…The antibacterial activity was conducted using the disc diffusion method as adopted from Thitilertdecha et al 11 and Su et al 12 To determine the minimum inhibition concentration (MIC) of each plant extract, selected Gram positive and Gram-negative micro-organisms where treated with 4 concentrations of plant extracts from serial dilution namely: 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml and 0.125 mg/ml. After serial dilutions were prepared, 6 mm filter paper discs were impregnated with the extract at each concentration.…”

Eugenyl acetate inhibits growth and virulence factors of drug‐resistant Acinetobacter baumannii

“…Plant extracts and their derived products are recognized as alternative candidates for the treatment of infections caused by pathogenic microbes. The extracts obtained from Holarrhena antidysenterica, Larrea tridentata , Polygonum cuspidatum, as well as plant‐derived compounds such as norwogonin from Scutellaria baicalensis , and terchebulin from Terminalia chebula have been previously reported to exhibit antibacterial activity against A. baumannii. Therefore, targeting plant products could be a suitable alternate to control infections caused by A. baumannii.…”