Antibacterial Action of Selenium-Enriched Probiotics Against Pathogenic Escherichia coli

Abstract

The purpose of this study was to evaluate the inhibitory activity of selenium-enriched probiotics against pathogenic Escherichia coli (E. coli) in vitro and in vivo. Escherichia coli was co-cultured in vitro with each probiotic strain individually, and a mixture of the four strains and its population was counted at various time points. We also collected a cell-free culture supernatant (CFCS) of each probiotic strain and the four-strain mix to examine their antibacterial activity, using the cylinder plate method. Results demonstrated that co-culture with probiotics significantly reduced the number of E. coli. The different sizes of the inhibition zones made by each CFCS proved that E. coli was inhibited by the metabolites of the probiotics. In vivo, Kunming mice were allocated to different groups supplemented with selenium-enriched and other probiotics. After 28 days, the mice were inoculated with pathogenic E. coli so that we could compare mortality rates and inspect other indexes of each treatment. The mortality of the group with selenium-enriched probiotics was the lowest. In addition, the organic antioxidant status improved, immunity was fortified, and the internal environment of the intestinal tract was enhanced with selenium-enriched probiotic supplementation. In conclusion, selenium-enriched probiotics can strongly antagonize pathogenic E. coli in vitro and in vivo.

Selenium nanoparticles inhibit Staphylococcus aureus growth

“…8 It was shown in that study that seleniumenriched probiotics (at a concentration of 0.509 µg selenium per gram of probiotics) inhibited the growth of E. coli after 96 hours in vitro. In vivo, mice were fed with and without selenium-enriched probiotics for 28 days and then inoculated with E. coli; mortality of the treated group was the lowest.…”

Preparation of Selenium-enriched Bifidobacterium Longum and its Effect on Tumor Growth and Immune Function of Tumor-Bearing Mice

“…Several studies have suggested that organic Se is usually less toxic and more bioavailable than inorganic Se (Brown et al, 2000). Recently, research showed that some microorganisms were capable of accumulating large amounts of Se and transforming inorganic Se into organic Se (Suhajda et al, 2000;Yang et al, 2009;Jin et al, 2012). Of these microorganisms, Se-enriched yeast has been studied extensively and is now commercially available as a nutritional Se supplement (Suhajda et al, 2000).…”

“…Of these microorganisms, Se-enriched yeast has been studied extensively and is now commercially available as a nutritional Se supplement (Suhajda et al, 2000). In addition, Lactobacillus was also found to have the ability to transform inorganic Se into organic compounds (Yang et al, 2009). In our previous study, Se was enriched to transformed B. longum carrying pBV22210-endostatin (B. longum-En).…”

Inhibition of various gram-positive and gram-negative bacteria growth on selenium nanoparticle coated paper towels

“…For example, selenium-enriched probiotics have been shown to strongly inhibit the growth of pathogenic Escherichia coli in vivo and in vitro. 9 The synthesized organoselenium compounds were shown to be as effective as penicillin at inhibiting Staphylococcus aureus growth in solution in vitro. 10 In addition, the growth of planktonic S. aureus was strongly inhibited in the presence of selenium nanoparticles.…”