Antiatherosclerotic Potential of Active Principle Isolated fromEugenia jambolanain Streptozotocin-Induced Diabetic Rats

Abstract

The aim of the present study was to investigate the antiatherosclerotic effect of active principle (FIIc) isolated from aqueous fruit pulp extract of Eugenia jambolana. Crude aqueous extract of E. jambolana was subjected to purification using chromatographic techniques which yielded purified active compound (FIIc). Purity of FIIc was tested by HPLC. Phytochemical investigation of FIIc by NMR, IR, and UV spectra showed that the purified compound is α-hydroxy succinamic acid. The streptozotocin- (STZ-) induced diabetic rats were fed atherosclerotic (Ath) diet containing 1.5 mL olive oil containing 8 mg (3, 20,000 IU) vitamin D2 and 40 mg cholesterol for 5 consecutive days. The STZ-induced diabetic rats receiving Ath diet were orally administered FIIc at doses of 10, 15, and 20 mg/kg, and results were compared with reference drug, that is, glibenclamide (600 μg/mg) and healthy control. 30-day treatment with FIIc resulted in significant (P < .001) improvement in blood glucose, serum lipid profile, apolipoproteins (Apo A1 and apoB100), and endothelial dysfunction parameters. Histomorphological studies also confirmed biochemical findings. Our results showed that FIIc has protective effect on hyperglycemia-induced atherosclerosis.

Herbal Anti-Hyperglycemic Compound Increases Expression of Glucose Transporter Molecules in Diabetic Rats

“…Diethyl Amino Ethyl cellulose-52 (DEAE-52) was used as the stationary phase. This technique was described in detail in the previous studies reported by Sharma et al [24,25]. From 1 kg of E. jambolana fruit, the pulp obtained was about 650 g. From this pulp the yield of lyophilized water extract obtained was about 10 g.…”

“…Purified active compound (FIIc) was given orally to group C at a dose of 20 mg/ kg of body weight/day and Pioglitazone was given as a standard drug orally at a dose of 20 mg/kg of body weight/day for 6 weeks to group D respectively for 6 weeks. This is the effective dose of FIIc standardized in previous studies reported by Sharma et al [24,25]. An equal volume of vehicle was given to healthy control group A and diabetic untreated group B at week 0 and at week 6.…”

“…6,426,826 dated 6th August 2002; Indian Product Patent no. 2,30,753 February 2009) from the fruit pulp of Eugenia Jambolana [24]. Hence this study was designed to estimate the effect of active herbal compound (FIIc) on peripheral insulin sensitivity by measuring the expression levels of GLUT-4, GLUT-8 and Kv 1.3 Potassium channel at mRNA and at protein level in streptozotocin induced diabetic rats.…”

Herbal Anti-Hyperglycemic Compound Improves Glycemic Control and Insulin Sensitivity in Diabetic Rats

“…The fruit may stimulate insulin secretion. The water extract of the fruit pulp also showed anti-hyperglycemic activity in normal and alloxan-induced diabetic rats [8] .…”

In vivoassessment of antidiabetic and antioxidative activity of natural phytochemical isolated from fruit-pulp ofEugenia jambolanain streptozotocin-induced diabetic rats

“…So far, no study has been reported about purification/isolation of active principle from aqueous fruit-pulp extract of E. jambolana. In our lab, we have isolated/purified an active principle from the aqueous extract of fruit-pulp of E. jambolana [13,14] …”