Antiapoptotic effects of vitamins C and E against cypermethrin‐induced oxidative stress and spermatogonial germ cell apoptosis

Abstract

Toxicological studies have demonstrated the relation between use of agrochemicals and fertility issues within males. Thus, the present study aimed to elucidate the propensity of cypermethrin (CYP) in bringing testicular germ cell apoptosis and effective attenuation by vitamins C and E in caprines. Reproductive toxicity of CYP was evaluated using histomorphological, cytological, and biochemical changes in the testicular germ cells in dose-dependent (1, 5, 10 μg/mL) and time-dependent (4, 6, 8 h) manner. Histological and ethidium bromide/acridine orange fluorescence staining exhibited that vitamins C and E (0.5 and 1.0 mM) successfully diminished the CYP-induced testicular germ cells apoptosis. CYP exposure along with vitamins C and E supplementation also resulted in significantly increased ferric reducing antioxidant power activity along with the antioxidant enzymes, namely catalase, superoxide dismutase, and glutathione-s-transferase, and decreased lipid peroxidation in testicular germ cells. Thus, vitamins C and E ameliorated CYP-induced testicular germ cell apoptosis, thereby preventing spermatogonial cells degeneration and male infertility.

In Vivo Attenuation of Alcohol- and Cadmium Chloride–Induced Testicular Toxicity Modulated by Silymarin in Male Wistar Rat

“…There was also mild derangement of testicular histology of the alcohol-treated rats following Silymarin administration, exhibiting the healing property of the drug on male infertility. The testicular cell atrophy observed in our study can be linked to cellular characteristic changes apoptosis induction caused by exposure to alcohol and cadmium chloride as was reported of previous studies [40][41][42]. Silymarin increased the serum testosterone, follicle-stimulating hormone, and luteinizing hormone level of the alcohol-treated rats.…”

Effects of heavy metals on reproduction owing to infertility

“…As oxidative stress‐mediated reproductive infertility induced by heavy metals like Cd 2+ and Pb 2+ is partially reversible, it can be cured by chelating agents and rehabilitation of individuals away from the heavy metal‐loaded environments. Therapeutic potentials of natural antioxidants like vitamin C‐rich lemon, [ 108 ] vitamin C and E, [ 6,123 ] N ‐acetyl‐ l ‐cysteine, [ 124 ] and so forth, are needed to be explored against heavy metal toxicity.…”

Quercetin mediated attenuation of cadmium‐induced oxidative toxicity and apoptosis of spermatogenic cells in caprine testes in vitro

“…Aggregates of testicular cells were centrifuged at 3000 rpm for 5 min using a GeNeI™ centrifuge. The supernatant was discarded and the pellet formed was resuspended in PBS (Bhardwaj et al ., 2018). The process was repeated thrice to get a debris‐free cell suspension.…”