In Vivo Attenuation of Alcohol- and Cadmium Chloride–Induced Testicular Toxicity Modulated by Silymarin in Male Wistar Rat

Abstract

The aim of the study is to investigate the in vivo attenuation of alcohol-and cadmium chloride-induced testicular toxicity modulated by Silymarin in male Wistar rats. A total of fifty-six (56) Wistar rats were used for this study and they were randomized into seven (7) groups of eight (8) rats each. Group 1 was control rats; Groups 2-7 served as the experimental groups. After 6 weeks treatment duration, the rats were euthanized, semen was collected for semen analysis, blood samples for testosterone, and FSH and LH assay determination, and left testes was harvested for histological analysis. One-way ANOVA was used to compare means at p-level < 0.05 was considered significant. Findings from this study have shown that alcohol and cadmium chloride adversely affected semen parameters, testosterone, and FSH and LH hormone milieu. Data also showed that Silymarin administration attenuated the adverse effect of alcohol and cadmium chloride on semen quality and hormones associated with reproductive functions. Hence, Silymarin mopped the effect of in vivo attenuation of alcohol and cadmium chloride testicular damage. The findings of this study have further established that alcohol and cadmium chloride adversely affected semen parameters, testicular alterations, and serum hormonal milieu. However, the effect was more significantly deleterious in rats exposed to cadmium chloride when compared to rats exposed to alcohol, subsequently alcohol-and cadmium chloride-induced degeneration of testicular tissues. Furthermore, Silymarin administration attenuated the adverse effect of alcohol on semen quality and hormones associated with reproductive functions.

Silymarin abrogates acrylamide‐induced oxidative stress‐mediated testicular toxicity via modulation of antioxidant mechanism, DNA damage, endocrine deficit and sperm quality in rats

“…Interestingly, the concomitant administration of SIL, a natural flavonoid, inhibited the ACR‐induced hormonal dysregulation in this study. The beneficial effects of natural products on testicular functions have been published in the existing literature (Belhan et al, 2021; Enebeli et al, 2021; Famurewa et al, 2020; Okkay et al, 2021). Silymarin is a natural products that has shown beneficial effect via exerting endocrine balance efficacy in several organs (Hussein et al, 2021; Yardım et al, 2021).…”

Modulatory role of welding fumes on serum zinc and copper levels and oxidative stress markers among welders: Considering smoking as a possible implication

“…The metals from our diet are assimilated into the blood, if blood levels are diminished, they are shifted into cells, if cellular levels are not enough, they are excreted if blood and cell levels are sufficient or overloaded. If the system cannot function in a proper manner, abnormal trace element ratios and concentrations might occur [23] . Examples of such commonest trace element disturbances are linked to serum copper and zinc concentrations present in welding fumes.…”

Myrrh Attenuates Gonadal Toxicity and DNA Fragmentation Induced by Ethanol in Male Rats

“…FSH acts on Sertoli cells to enhance sperm production, while LH binds to Leydig cells to promote testosterone synthesis (MacLean et al, 2022). Previous research has indicated that alcohol consumption may negatively affect the efficiency of the endocrine system, including the HPG axis, thus inhibiting the production of LH and testosterone (Enebeli et al, 2022).…”