Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model

Öztürk, Şamil; Sönmez, Pınar Kılıçaslan; Özdemir, İlhan; Topdaği, Yunus Emre; Tuğlu, Mehmet İbrahim

doi:10.17826/cumj.573200

Cited by 4 publications

(5 citation statements)

References 31 publications

(19 reference statements)

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“…TUNEL stainings were scored semi-quantitatively to determine the number of positive staining, none (-), weak (+), moderate (++), high (+++), very high (++++). These analyses were performed in 2 sections for each animal, at a magnification rate of X40 for at least 10 different regions per section (11,12).…”

Section: Tunel Methods and Apoptotic Activitymentioning

confidence: 99%

“…Following this step, the sections were incubated with a polyclonal nuclear factor Kappa-B (NF-κB p50, ab323360), tumor necrosis factor (TNF-α, ab194403), then the sections were incubated with biotinylated anti-mouse Immunoglobulin-G (DAKO LSAB 2 Kit, İnvitrogen). Following this step, they were stained with ABC complex and then with Mayer's Hematoxylin (11,12).…”

Section: Immunohistochemical Staining With Tnf-α and Nf-κbmentioning

confidence: 99%

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Investigation of Apoptotic and Inflammatory Activity in Liver Tissue of Rats Fed with Clam (Pecten maximus, Linnaeus 1758)

İrkin

2021

Revista Romana De Medicina De Laborator

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One of the most important threats for living things in aquatic ecosystems is environmental pollution. The changes in water quality caused by environmental pollution also reduce the quality of life for organisms in the environment. Among these, the crustaceans which are most affected by the negative changes of environment, fed by the filtration method, are seen as pollution indicator. The consumption of these creatures reaches all steps of the pyramid, especially humans, through the food chain. People who frequently use seafood in their diets may be affected by these negative changes. Heavy metal contents of the clams obtained from Dardanelles were determined by the ICPOES. Twenty-four female Wistar albino rats were fed for 30 days with the experimental diet using clams (Pecten maximus), which was dried and formed into pellets and added to the food in certain proportions. At the end of the study, the subjects were sacrificed under anesthesia, liver tissues were taken, and histochemical examination was performed. TUNEL method was performed to detect apoptotic activity, and immunohistochemical staining with TNF-α and NF-κB antibodies to determine inflammation. Concluding from the results, it was observed that the degeneration of vital digestive system tissues such as liver was inevitable in living creatures that frequently consume seafood obtained from unhealthy environment in their daily diets. The high analysis values of the heavy metal (P. maximus) in food additive can be considered as a reason for histopathological results.

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Section: Tunel Methods and Apoptotic Activitymentioning

confidence: 99%

Section: Immunohistochemical Staining With Tnf-α and Nf-κbmentioning

confidence: 99%

Investigation of Apoptotic and Inflammatory Activity in Liver Tissue of Rats Fed with Clam (Pecten maximus, Linnaeus 1758)

İrkin

2021

Revista Romana De Medicina De Laborator

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“…After the tissue samples were placed in deionized water two times and for 2 minutes, they were kept in Mayer's hematoxylin for 5 minutes for counterstaining and washed in tap water for 10 minutes. Then, samples were passed through xylene and graded alcohols and covered with a coverslip using entellan (Bio Mount, Bio-Optica) [30].…”

Section: Immunohistochemically Stainingmentioning

confidence: 99%

“…Then dyeing was done with DAB, and background staining was done with Mayer's hematoxylin. TUNEL positive cells were detected by the blind method, and the averages were evaluated statistically [30].…”

Section: Tunel Assaymentioning

confidence: 99%

Protective Effects of Quercetin/Moss Extract (Homalothecium Sericeum Hedw.) Combination on STZ-Induced Diabetic Rats

Yayıntaş¹

2022

J Diabet Res Rev Rep

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Diabetes Mellitus (DM) is the most common chronic endocrine disorder. Although new drugs and administration techniques for diabetes were developed, the nature of complications that occur chronically and reduce patients’ quality of life have not been entirely determined. In our study, 35 male rats were used. A single dose of 40 mg/kg streptozotocin was injected intraperitoneal into 28 rats, and a total of five groups were formed. The diabetes model was performed by applying 40mg/kg STZ and was not established in the subjects in the control and citrate buffer groups with STZ solvent. The quercetin and moss extract were given by gavage for three weeks. The liver and pancreas tissues were examined by histochemical and immunohistochemical methods. Ki-67 (proliferation marker), antinuclear factor kappa (NF-κB), and interleukin 6 (IL-6) immunohistochemically staining revealed significant differences in inflammation and regeneration in the liver and pancreas tissues. In addition, the TUNEL staining method was used to determine apoptosis. Positivity was significant between the treatment and diabetes groups. It was determined that the agents used as treatment changed serum superoxide dismutase (SOD), malondialdehyde (MDA), C-peptide, insulin, and glucose levels.

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“…Following this step, the sections were incubated with the ABC complex (DAKO LSAB 2 Kit). For background staining, Mayer's Hematoxylin (Gürpınar et al, 2012;Öztürk et al, 2019).…”

Section: Immunohistochemically Examinationmentioning

confidence: 99%

Kirlenmiş Alanlardan Toplanan Akdeniz Midyesi'nin Ratlarda İmmünohistokimyasal Metotla Hepatotoksik Etkisi

Çakına

İrkin

Özdemir³

et al. 2021

Acta Aquatica Turcica

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Mussels (Mytilus galloprovincialis) are aquatic organisms that can accumulate all the factors that pollute the fresh and saltwater environment. Although the Dardanelles has been exposed to environmental pollution for many years, it is also an important region for crustaceans. We aimed to investigate the histopathological changes in the liver parenchyma which is an important gland of the digestive system by feeding these mussels to rats. Twenty-four male Wistar albino were used in the study. The first group (control): fed with standard rat feed, the second group (experiment 1): 4/5 mussel + 1/5 standard rat feed daily; third group (experiment 2): 4/5 mussel + 1/5 standard rat feed every other day, the fourth group (experiment 3): groups were formed with 4/5 mussel + 1/5 standard rat feed every three days. All liver tissue samples taken from the experimental and control groups were stained with hematoxylin-eosin, and immuohitochemically staining inflammatory marker TNF-α and NF-κB after routine histopathological follow-up and analyzed with a light microscope image analysis system. It was observed that mononuclear cells caused inflammation of portal areas, increased sinusoidal dilatation and congestion and degeneration due to vacuolization in hepatocytes in the liver parenchyma of mussel-fed rats. Besides, immunohistochemical staining, TNF-α, and NF-κB immunoreactivity were observed in the liver cells of especially in the second group of rats. As a result, it has been shown that the consumption of mussels obtained and marketed without considering environmental pollution may trigger important digestive system organs of liver diseases.

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Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model

Cited by 4 publications

References 31 publications

Investigation of Apoptotic and Inflammatory Activity in Liver Tissue of Rats Fed with Clam (Pecten maximus, Linnaeus 1758)

Investigation of Apoptotic and Inflammatory Activity in Liver Tissue of Rats Fed with Clam (Pecten maximus, Linnaeus 1758)

Protective Effects of Quercetin/Moss Extract (Homalothecium Sericeum Hedw.) Combination on STZ-Induced Diabetic Rats

Kirlenmiş Alanlardan Toplanan Akdeniz Midyesi'nin Ratlarda İmmünohistokimyasal Metotla Hepatotoksik Etkisi

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