Antiallergic Effects of Lactobacillus pentosus Strain S-PT84 Mediated by Modulation of Th1/Th2 Immunobalance and Induction of IL-10 Production

Abstract

Background: Many types of fermented food are consumed in Japan. Although some are produced by plant-origin lactic-acid bacteria (LAB) fermentation, the physiological functions of such bacteria remain unclear. We therefore isolated LAB of plant origin from Kyoto pickles and determined the immunological activity of heat-killed preparations of plant-origin LAB. Methods: The Lactobacillus pentosus strain S-PT84 was selected from among 16 LAB of plant origin as the strongest interleukin (IL)-12-inducing strain. IL-12- and IL-10-inducing activities were determined with macrophages from BALB/c mice. The in vivo immunomodulating effect of S-PT84was determined with BALB/c mice fed S-PT84. The antiallergic activity of S-PT84 was examined in ovalbumin (OVA)/alum-administered BALB/c mice. Results: The L. pentosus strain S-PT84 induced production of both IL-12 and IL-10 in vitro. S-PT84 enhanced splenic natural-killer activity and modulated the T helper (Th) type 1/type 2 balance toward a Th1-dominant state. In the OVA-induced allergy model, orally administered S-PT84 lowered serum IgE levels and suppressed active cutaneous anaphylaxis reaction and splenic IL-4 production. IL-10 production from splenocytes of OVA-immunized mice was upregulated by feeding S-PT84. Conclusions: Despite heat-killing, S-PT84 exhibited antiallergic effects by modulating the Th1/Th2 balance and inducing regulatory T cells. The L. pentosus strain S-PT84, which is of plant origin and isolated from a traditional Japanese food, is expected to be useful for treatment of many immune diseases including allergies, tumors, infectious diseases and auto-immune diseases.

Effects of Bifidobacterium breve on inflammatory gene expression in neonatal and weaning rat intestine

“…Although lactobacilli and bifidobacteria are often used as probiotics, their functions seem to be different. Nonaka et al suggested that lactobacilli modulate the Th1/Th2 immune balance toward Th1 reactions (8). Shima et al reported that the Lactobacillus casei strain Shirota enhances the expression of genes involved in defense/ immune functions and in lipid metabolism, whereas the B. breve strain Yakult downregulates the expression of many genes (26).…”

“…In numerous human trials, although the precise mechanisms have not been determined, intake of lactobacilli and bifidobacteria has been reported to be beneficial with respect to stool frequency and stool consistency (2,3). The potential benefit of probiotics in the modulation and regulation of the immune response has also been reported in both humans and animals (4)(5)(6)(7)(8).…”

“…Gene expression in the rat colon, small intestine, and spleen was examined by microarray and real-time PCR after feeding 5 × 10 8 colony-forming units/animal B. breve to rat pups during the newborn (days [1][2][3][4][5][6][7][8][9][10][11][12][13][14] or weaning (days 21-34) period.…”

Tetragenococcus halophilus MN45 Isolated from Miso Inhibits IgE Production

“…Recently, it has been reported that IL-10 and TGF-β produced by regulatory T cells suppress allergic inflammatory responses (Hayashi et al, 2009;Nonaka et al, 2008). Therefore, studies on MN45 induction of anti-inflammatory cytokines and anti-allergic effect are now in progress.…”

Oral Administration of Lactic Acid Bacteria Isolated from Traditional South Asian Fermented Milk `Dahi' Inhibits the Development of Atopic Dermatitis in NC/Nga Mice

“…Some of antiallergic LAB were isolated from traditional fermented foods, such as Kyoto pickles ( 15 ), soy sauce moromi ( 16 ), and Mongolian fermented milk ( 17 ). Traditional fermented foods-derived LAB are able to exert certain immunomodulatory effects, but there are not many reports concerning their beneficial effects on AD.…”