Antialgal effects of α-linolenic acid on harmful bloom-forming Prorocentrum donghaiense and the antialgal mechanisms

Abstract

Harmful algal blooms (HABs) induced by Prorocentrum donghaiense occur frequently and cause a serious threat to the marine ecosystem. In this study, antialgal effects of α-linolenic acid (ALA) that is generally extracted from diverse macroalga on P. donghaiense were investigated. Specifically, the growth, cellular morphology and ultrastructure, reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), cytochrome C (Cyt-C), and caspase-9,3 activity of untreated and treated P. donghaiense were investigated. The results showed that ALA significantly inhibited the growth of P. donghaiense. Under ALA exposure, the cellular morphology and ultrastructure were damaged. ALA also induced ROS overproduction in the algal cells, decreased MMP, induced Cyt-C release, and activated caspase-9,3, which strongly relates to algal apoptosis. In summary, this study revealed the responses of morphology and physiology of P. donghaiense when exposed under ALA, and shows the potential of biotechnology on controlling P. donghaiense.

Essential oils from Moroccan plants as promising ecofriendly tools to control toxic cyanobacteria blooms

“…Accordingly, the search for an ecofriendly alternative approach based on the isolation of new bioactive molecules with potent effects against toxic cyanobacteria especially M. aeruginosa becomes an important issue in the field of water environment protection. The use of natural products is now emerging as one of the leading solution to prevent the development of microalgae (Li et al, 2016;Tazart et al, 2018;Wang et al, 2018;Zerrif et al, 2018). The essential oils (EOs) are among natural plant products that are obtained from many aromatic and medicinal plants.…”

Inhibitory effect and mechanism of three macrophytes extract on Microcystis aeruginosa growth and physiology

“…Many previous studies have identified oxidative damage as one of the important allelopathic and toxicological mechanisms of macrophyte allelochemicals on phytoplankton and cyanobacteria (Hong et al ., 2008; Zhao et al ., 2013; Mohamed, 2017; Wang et al ., 2018a; Jiang et al ., 2019; Mowe et al ., 2019; Kang et al ., 2020). In our work, several physiological common indicators have been identified regarding cells responses to the treatment.…”

Synthesis, Characterization, and Applications of Rare-Earth-Based Complexes with Antibacterial and Antialgal Properties

“…Chlorella (GY-D25 Chlorella vulgaris) was purchased from Shanghai Guangyu Biological Technology Co., Ltd. and kept in our laboratory, and the diatom was provided by LuoYangtuents Ship Material Research Institute, Qingdao monitoring station. This microalga was cultured in f/2 medium (Guillard 1975) prepared with seawater in a constant temperature incubator at 20 ± 3 °C with cool-white light of 100 μmol photons m –2 s –1 and a light/dark ratio of 12 h/12 h. The cultures were shaken three times per day. , …”

“…This microalga was cultured in f/2 medium (Guillard 1975) prepared with seawater in a constant temperature incubator at 20 ± 3 °C with cool-white light of 100 μmol photons m −2 s −1 and a light/dark ratio of 12 h/12 h. The cultures were shaken three times per day. 39,40 2.6.2. Effect of the Complexes on the Growth of Chlorella.…”