Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin‐Induced Premature Aging in Adult Mice

Abstract

The roots of Vicatia thibetica de Boiss are a kind of Chinese herb with homology of medicine and food. This is the first report showing the property of the extract of Vicatia thibetica de Boiss roots (HLB01) to extend the lifespan as well as promote the healthy parameters in Caenorhabditis elegans (C. elegans). For doxorubicin- (Doxo-) induced premature aging in adult mice, HLB01 counteracted the senescence-associated biomarkers, including P21 and γH2AX. Interestingly, HLB01 promoted the expression of collagen in C. elegans and mammalian cell systemically, which might be one of the essential factors to exert the antiaging effects. In addition, HLB01 was also found as a scavenger of free radicals, thereby performing the antioxidant ability. Lifespan extension by HLB01 was also dependent on DAF-16 and HSF-1 via oxidative stress resistance and heat stress resistance. Taken together, overall data suggested that HLB01 could extend the lifespan and healthspan of C. elegans and resist Doxo-induced senescence in mice via promoting the expression of collagen, antioxidant potential, and stress resistance.

Nicandra physalodes Extract Exerts Antiaging Effects in Multiple Models and Extends the Lifespan of Caenorhabditis elegans via DAF-16 and HSF-1

“…The approval number was 20211009013. Mouse assays were conducted according to the previously reported procedures [ 12 , 17 ]. Metformin served as a positive control.…”

“…Thrashing Assay and Pharyngeal Pumping Assay. The thrashing assay and pharyngeal pumping assay were performed following the previously described methods [12]. The experiments were performed with reference to the lifespan assay.…”

“…With the aim of identifying effective and safe antiaging agents, we screened 836 Chinese herbal medicine extracts through Caenorhabditis elegans ( C. elegans ) lifespan experiments [ 12 ]. After three rounds of lifespan screening experiments, Nicandra physalodes extract (HL0285) ( Figure 1(a) ) was selected for the study because of its apparent lifespan extension activity.…”

Longevity-promoting properties of ginger extract in Caenorhabditis elegans via the insulin/IGF-1 signaling pathway

“…Numerous studies have reported that plant extracts or natural phytochemicals improve the lifespan of worms and mammals. 4,28 To examine the effect of GE on the lifespan of C. elegans, the worms were treated with different concentrations of GE. As shown in (Fig.…”