Antiageing properties of Damaurone D in <i>Caenorhabditis elegans</i>

Kim, Yong Seong; Han, Young Taek; Jeon, Hoon; Seok, Dong

doi:10.1111/jphp.12979

Cited by 10 publications

(4 citation statements)

References 34 publications

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“…The use of RNAi to inhibit the transcription factor DAF-16 reduced lifespan and reduced the expression of the sod-3. In another study, the isoprenylated aurone damaurone D was tested for its longevity-promoting effect in C. elegans [180]. Similar to other studies, the compound increased the lifespan of wild-type nematodes, reduced ROS production, increased CAT and SOD activity, increased sod-3 gene expression, and also improved stress tolerance under oxidative, thermal, and osmotic stress via the upregulation of hsp-16.2 and sod-3.…”

Section: Plant Compounds and Longevitymentioning

confidence: 69%

Plant-Derived Bioactives and Oxidative Stress-Related Disorders: A Key Trend towards Healthy Aging and Longevity Promotion

Azzini²,

et al. 2020

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Plants and their corresponding botanical preparations have been used for centuries due to their remarkable potential in both the treatment and prevention of oxidative stress-related disorders. Aging and aging-related diseases, like cardiovascular disease, cancer, diabetes, and neurodegenerative disorders, which have increased exponentially, are intrinsically related with redox imbalance and oxidative stress. Hundreds of biologically active constituents are present in each whole plant matrix, providing promissory bioactive effects for human beings. Indeed, the worldwide population has devoted increased attention and preference for the use of medicinal plants for healthy aging and longevity promotion. In fact, plant-derived bioactives present a broad spectrum of biological effects, and their antioxidant, anti-inflammatory, and, more recently, anti-aging effects, are considered to be a hot topic among the medical and scientific communities. Nonetheless, despite the numerous biological effects, it should not be forgotten that some bioactive molecules are prone to oxidation and can even exert pro-oxidant effects. In this sense, the objective of the present review is to provide a detailed overview of plant-derived bioactives in age-related disorders. Specifically, the role of phytochemicals as antioxidants and pro-oxidant agents is carefully addressed, as is their therapeutic relevance in longevity, aging-related disorders, and healthy-aging promotion. Finally, an eye-opening look into the overall evidence of plant compounds related to longevity is presented.

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Section: Plant Compounds and Longevitymentioning

confidence: 69%

Plant-Derived Bioactives and Oxidative Stress-Related Disorders: A Key Trend towards Healthy Aging and Longevity Promotion

Azzini²,

et al. 2020

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“…With the oleracones D-F (1-3) in hand, we examined their effects on the longevity of nematodes. To test whether oleracones exhibit lifespan-extension activity, we performed a lifespan assay using wild-type N2 nematodes at 20 • C, as described previously [21]. As shown in Figure 2A,B, all of the oleracones tested could prolong the lifespan of the nematodes under standard culture conditions, although the effectiveness of each compound was somewhat variable.…”

Section: Scheme 1 Retrosynthetic Analysis Of Oleracones D (1) F (2) and E (3)mentioning

confidence: 97%

Synthesis and Evaluation of the Lifespan-Extension Properties of Oleracones D–F, Antioxidative Flavonoids from Portulaca oleracea L.

et al. 2019

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Plant-derived antioxidants have been widely used as supplementary health foods, as well as having been regarded as drug candidates for aging and aging-associated diseases. Oleracones, a novel series of flavonoids isolated from Portulaca oleracea L., possess potent antioxidative activities and are expected to exhibit therapeutic potential on the aging process. The current paper describes the concise sequential synthesis of oleracones D–F. Oleracones D and F were efficiently synthesized via selective intramolecular oxa-Michael addition from oleracone E. In addition, we investigated their possible lifespan-extension properties using Caenorhabditis elegans, which is excellently suited as an experimental model to study aging. A significant longevity effect was observed when nematodes were grown with 20 μM of oleracone E.

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“…Lifespan assay was done using a previously described method (5). For the thermotolerance assay, the worms were incubated at 36℃ for 1 hour and their survival rates were scored every hour on the 1 st , 3 rd , 5 th , 9 th , and 11 th days of adulthood, respectively.…”

Section: Stress Tolerance and Lifespan Assaysmentioning

confidence: 99%

“…The lipofuscin level of wild-type worms and the GFP fluorescence of transgenic worms were observed as described previously (5). Briefly, the test worms were anesthetized with 2% sodium azide before microscopy observation, and fluorescence images were captured.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Motherwort (Leonurus japonicus Houtt.) extends the lifespan and healthspan of Caenorhabditis elegans via sir-2.1 and daf-16 activation

Yang,

Yoon,

Jeon

et al. 2023

J Herbmed Pharmacol

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Introduction: As a traditional medicine, the aerial parts of Leonurus japonicus Houtt. (Lamiaceae), aka motherwort, have been extensively used to treat gynecological diseases. The current study was designed to investigate the longevity properties of the methanolic extract of L. japonicus (MLJ) using Caenorhabditis elegans model system. Methods: The longevity effect of MLJ was determined by lifespan assay. Lipofuscin accumulation, thermotolerance, and body movement were measured to test the effects on the healthspan. The antioxidant capacity of MLJ was investigated by analyzing antioxidant enzyme activities, intracellular reactive oxygen species (ROS) levels, and the survival rate against oxidative stress conditions. Pharyngeal pumping rate and body length were observed to determine the effect of MLJ on aging-related factors. Transcriptional activity of daf-16 was observed under fluorescence microscopy using a transgenic mutant carrying DAF-16::GFP transgene. Results: MLJ could significantly prolong the median and maximum lifespan of worms. In addition, MLJ reduced the accumulation of lipofuscin in aged worms and delayed the age-dependent decrease in locomotion and thermotolerance suggesting its beneficial role in the healthspan. Also, MLJ increased the stress resistance of worms against oxidative stress and decreased intracellular ROS generation by up-regulating the activities of antioxidant enzymes. Additional genetic studies showed that MLJ failed to prolong the lifespan of worms lacking daf-2, age-1, daf-16, and sir-2.1 genes. Moreover, in the presence of MLJ, the nuclear translocation of daf-16 was significantly increased. Conclusion: Collectively, our results demonstrate that the anti-aging properties of MLJ might be attributed to sir-2.1 and insulin/IGF signaling-dependent daf-16/FOXO activation.

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Antiageing properties of Damaurone D in Caenorhabditis elegans

Abstract: These results suggest that DaD has beneficial effects on the longevity, and thus it can be a valuable plant origin lead compound for the development of nutraceutical preparations targeting ageing and ageing-related diseases.

Cited by 10 publications

References 34 publications

Plant-Derived Bioactives and Oxidative Stress-Related Disorders: A Key Trend towards Healthy Aging and Longevity Promotion

Plant-Derived Bioactives and Oxidative Stress-Related Disorders: A Key Trend towards Healthy Aging and Longevity Promotion

Synthesis and Evaluation of the Lifespan-Extension Properties of Oleracones D–F, Antioxidative Flavonoids from Portulaca oleracea L.

Motherwort (Leonurus japonicus Houtt.) extends the lifespan and healthspan of Caenorhabditis elegans via sir-2.1 and daf-16 activation

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