Antiadhesive effects of mitomycin C and streptopeptidase A in rats with intraperitoneal adhesions

Abstract

Intraabdominal adhesion formation is a frequent problem after major abdominal surgery. For many years, there have been various attempts to decrease adhesions by using systemic and local drugs and mechanical barriers. In this study we aimed to evaluate the antifibrinolytic antiadhesive effects of mitomycin C (MMC) and streptopeptidase A (SA) against intraabdominal adhesions. Forty-eight rats were divided into six groups, each with eight rats. Group 1 (sham group) rats were laparotomized by transverse incision only. In Group 2 (laparotomy and talcum powder), 2 ml talcum powder was scattered equally onto the intestinal surface after laparotomy. Group 3 (SA only), 2 g SA was introduced onto the intestinal surface. Group 4 (talcum powder and SA), 2 ml talcum powder was scattered onto the intestinal surface and then 2 g SA was applied on the same area. Group 5 (MMC only), 2 ml MMC was introduced onto the intestinal surface. Group 6 (talcum powder and MMC), 2 ml talcum powder was scattered onto intestinal surface and then MMC was applied onto same area. We assessed adhesion grades macroscopically, as well as, hydroxproline levels biochemically. Macroscopicaly, the number of rats with moderate or severe adhesions was significantly higher in the control group than all other groups (P < 0.05). SA and MMC groups had only mild adhesions. No intraabdominal problem was detected in rats with SA or MMC. Hydroxyproline (HP) levels were significantly higher in control group than all other groups (P < 0.05). There was no statistical significance between the rats with SA and MMC (P > 0.05) according to the HP measurements. MMC and SA may have potential antiadhesive effects. Both substances could be beneficial against adhesion formation after laparotomies.

P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3

“…Blockers were injected intraperitoneally every day for 2 weeks after the injury. Induction of adhesions by talc powder was performed as described previously 48 .…”

Comparison of Substances Used for Prevention of Intra-Abdominal Adhesions: An Experimental Study in Rats

“…4,9 Nonsteroid anti-inflamatuar ilaçlar (NSAİD), glikokortikoidler, progesteron/östrojen, düşük molekül ağırlıklı heparin, fibrinolitikler, metilen mavisi, pentoksifilin, streptokinaz, antibiyotikler, prostoglandin sentez inhibitörleri gibi farmakolojik maddeler, mitomisin-C gibi kemoterapötik ajanlar, %4 icodekstrin solüsyonu, hyaluronan, fosfolipidler gibi adjuvan maddeler, karboksimetilsellüloz, okside edilmiş selüloz ve politetrafloroetilen gibi solid bariyerler sıklıkla kullanılan antiadezif maddelerdir. 3,4,7,8,10,[18][19][20][21][22] Turkiye Klinikleri J Med Sci 2012;32 (2) Günaydın ve ark.…”

“…3,4,7 Bu alanda yapılan çalışmalarla ilgili literatür gözden geçirildiğinde solid, jel ve solüsyon bariyerlerinin yanı sıra proliferasyonu, inflamasyonu, fibrin yapımını önleyici maddeler ve kemoterapötik ilaçların kullanıldığı görülmektedir. 3,4,[7][8][9][10][11] Bu çalışmanın amacı, söz konusu malzemelerden güncel olarak kullanılan kompozit bir jel olan polietilenoksid (PEO) ve anyonik polisakkarit yapıda viskoelastik jel olan sodyum-karboksimetilselluloz (CMS); yağlayıcı ve kaydırıcı visköz jel olan hyaluronan (HA); adezyon önleyici olarak daha önce denenmemiş yüzey aktif bir ajan ve yara iyileşmesine ve bakterilere etkili bir madde olan oktenidin dihidroklorid (ODH) ve bu maddeyle sinerjistik etki gösteren fenoksietanol (FE); ve yüzey gerilimini azaltan, lipid ve proteinden oluşan kompleks bir madde olan surfaktanın (SFT) karın içi yapışıklık önlemedeki etkinliklerini ve birbirinden farklılıklarını deneysel olarak değerlendirmektir.…”

Role of Single-Dose Clostridiopeptidase A Collagenase in Peritoneal Adhesions

“…In the cited study, talcum powder was spread onto the intestinal surfaces to ensure peritoneal adhesion, and 2 g of streptopeptidase A was applied onto the surfaces 2 min later. In the cited study, streptopeptidase A had antiadhesive effects [25]. …”