Antiadenovirus Activities of Several Classes of Nucleoside and Nucleotide Analogues

Naesens, Lieve; Lenaerts, Liesbeth; Andrei, Gracíela; Snoeck, Robert; Beers, Danielle Van; Holý, Antonı́n; Balzarini, Jan; Clercq, Erik De

doi:10.1128/aac.49.3.1010-1016.2005

Cited by 141 publications

(124 citation statements)

References 29 publications

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“…Cidofovir is perhaps the most effective antiadenoviral agents, 21,22 which has been used to treat infections of adenoviruses like type 5. 23,24 Cidofovir also has a broad spectrum of activity against other viruses such as human herpes virus, adenovirus, polyomavirus, and human poxviruses.…”

Section: Effect Of Cidofovir On Gene Expressionmentioning

confidence: 99%

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Rathod

Vangipuram²,

Krishnan

et al. 2006

Int J Obes

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Objective: Human adenovirus Ad-36 causes adiposity in animal models and shows association with human obesity. Ad-36 enhances differentiation of 3T3-L1 and human preadipocytes, without cell lysis, a characteristic that may contribute to its adipogenic effect observed in vivo. Ad-2, another human adenovirus is nonadipogenic in animals and in 3T3-L1 cells and shows no correlation with human obesity. The objective of this study was to determine the adipogenic roles of viral mRNA and DNA, which may explain the differential effects of Ad-36 and Ad-2 on preadipocyte differentiation. Methods: This study determined the duration of selected Ad-36 gene expression in 3T3-L1 cells, and the effect on preadipocytes differentiation, when Ad-36 gene expression was attenuated by Cidofovir, an antiadenoviral agent. Results:The results showed that Ad-36, but not Ad-2, expresses viral mRNA. Ad-36 gene expression peaked at 2-4 days postinoculation and very low levels persisted after day 7. Despite the viral mRNA expression, Ad-36 infection of 3T3-L1 cells was abortive as indicated by a progressive decrease in viral DNA quantity. Attenuation of Ad-36 mRNA expression by Cidofovir reduced the adipogenic effect of the virus. Conclusion: In conclusion, viral mRNA expression, although transient, is a prerequisite for enhancing differentiation of preadipocytes by Ad-36. Viral DNA replication was not required for the effect. This is the first evidence for the role of gene expression of an adipogenic human virus in enhancing preadipocytes differentiation. This study provides the basis for further understanding novel regulatory modulators of preadipocytes differentiation.

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Section: Effect Of Cidofovir On Gene Expressionmentioning

confidence: 99%

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Rathod

Vangipuram²,

Krishnan

et al. 2006

Int J Obes

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“…Não há tratamento específi-co nem profilático para evitar o contágio interhumanos ou a transmissão para o segundo olho do paciente. Alguns agentes como o ganciclovir tem mostrado benefícios potenciais para esta condição (10)(11)(12)(13)(14) e por esta razão foi realizado este estudo. O ganciclovir tópico já é comercializado em vários países da Europa, Ásia, África e América do Sul para tratamento de herpes ocular.…”

Section: Introductionunclassified

Uso de ganciclovir 0,15% gel para tratamento de ceratoconjuntivite adenoviral

Yabiku¹,

Yabiku²,

Bottós³

et al. 2011

Arq. Bras. Oftalmol.

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Comitê de Ética em Pesquisa da UNIFESP/EPM -CEP 2042/08 RESUMOObjetivos: Determinar a efetividade e a toxicidade do ganciclovir 0,15% gel no tratamento de ceratoconjuntivites adenovirais e na prevenção de complicações tais como infiltrados corneanos, membranas ou pseudomembranas conjuntivais. Métodos: Ensaio clínico duplo-cego, intervencionista, randomizado. Os 33 pacientes com diagnóstico clínico de ceratoconjuntivite adenoviral com início dos sintomas há menos de cinco dias foram randomizados em dois grupos: Grupo 1 (tratamento) com 19 pacientes que usaram ganciclovir e Grupo 2 (controle) com 14 pacientes que usaram lágrima artificial sem conservante. Todos pacientes responderam a um questionário de sinais e sintomas e foram submetidos a um exame oftalmológico. No 6º dia de tratamento responderam ao mesmo questionário por telefone e no 10º dia foram reavaliados pelo mesmo examinador e responderam novamente ao questioná-rio. Os sinais e sintomas foram comparados. Para análise estatística foi utilizado os testes T de Student, Mann-Whitney e Wilcoxon, com significância estatística p<0,05. Resultados: Tendência de melhor resposta no grupo tratamento em relação à percepção pelos pacientes, além da melhora mais rápida desse grupo em relação ao grupo controle (p=0,26). Houve menor transmissão para o olho adelfo (p=0,86) e para pessoas do convívio (p=0,16) no grupo tratamento. Comparando os dois grupos não houve diferença estatística em relação aos sintomas e sinais da conjuntivite. Comparando isoladamente cada grupo entre o pré-tratamento e no decorrer do tratamento, observou-se melhora estatisticamente significativa da dor, prurido e fotofobia apenas no grupo tratamento. Ganciclovir não mostrou toxicidade e teve maior tolerância pelos pacientes. Não houve diferença significativa no aparecimento de complicações da conjuntivite entre os dois grupos. Conclusões: O estudo evidenciou uma tendência à melhora mais rápida dos sinais e sintomas dos pacientes tratados com ganciclovir em relação ao grupo controle, porém não estatisticamente significante. Esses resultados precisam ser confirmados por estudos adicionais, com maior número de pacientes e um seguimento mais extenso. Clinical Trails.gov: NCT01349452

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“…Other agents such as Ganciclovir, ribavirin [76], vidarabine and foscarnet [93] have failed to show consistent activity against adenovirus in vitro for various reasons. Ganciclovir requires activation by phosphorylation to monophosphate form by viral kinase and since adenoviruses, in contrast to members of the herpesviridae, lack viral thymidine kinase, the efficacy of ganciclovir is modest in comparison to cidofovir [93].…”

Section: Antiviral Agentsmentioning

confidence: 99%

“…Ganciclovir requires activation by phosphorylation to monophosphate form by viral kinase and since adenoviruses, in contrast to members of the herpesviridae, lack viral thymidine kinase, the efficacy of ganciclovir is modest in comparison to cidofovir [93].…”

Section: Antiviral Agentsmentioning

confidence: 99%

The Repertoire of Adenovirus in Human Disease: The Innocuous to the Deadly

Khanal¹,

Ghimire²,

Dhamoon³

2018

Preprint

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Abstract:Adenovirus is a family of double stranded DNA viruses that are a significant cause of upper respiratory tract infections in children and adults. Less commonly, the adenovirus family can cause a variety of gastrointestinal, ophthalmologic, genitourinary, and neurologic diseases. Most adenovirus infections are self-limited in the immunocompetent host and are treated with supportive measures. Fatal infections can occur in immunocompromised patients and less frequently in the healthy. Adenoviral vectors are being studied for novel biomedical applications including gene therapy and immunization. In this review we will focus on the spectrum of adenoviral infections in humans.

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Antiadenovirus Activities of Several Classes of Nucleoside and Nucleotide Analogues

Cited by 141 publications

References 29 publications

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Uso de ganciclovir 0,15% gel para tratamento de ceratoconjuntivite adenoviral

The Repertoire of Adenovirus in Human Disease: The Innocuous to the Deadly

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