Antiacetylcholinesterase and antioxidantent-Kaurene diterpenoid, melissoidesin fromIsodon wightii(Bentham) H. Hara

Abstract

The ent kaurene diterpenoid, melissoidesin was isolated from the acetone extract of the leaves of Isodon wightii and the structure was designated as 3beta, 11beta, 15beta-trihydroxy-6alpha-acetoxy-ent-kaur-16-ene based on spectral data and previous reports. Melissoidesin isolated from the acetone extract of leaves showed potent antiacetylcholinesterase activity and the IC(50) value was observed as 215 microg mL(-1). DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of melissoidesin was significant and the IC(50) value was 138 microg mL(-1). The significant reducing property of the melissoidesin was stronger in high concentration. IC(50) value of melissoidesin on hydroxyl radicals and metal chelation was observed as 99 and 143 microg mL(-1), respectively. The 50% inhibitory concentration of melissoidesin on lipid peroxidation was calculated as 133 microg mL(-1). These findings indicate that ent kaurene diterpenoid, melissoidesin was promising antiacetylcholinesterase and antioxidant which can be used as food and drug preparations.

Chemical composition and biological activity of the essential oil from the wood of Pinus heldreichii Christ. var. leucodermis

“…It is well known that phenolic compounds are strong antioxidant agents, but recently it has been published that essential oils rich in nonphenolic compounds may also exhibit antioxidant properties (Kordali et al 2005). It has been published in recent literature, that compounds like limonene, α-pinene, β-pinene and kaurene type diterpenoids, which are among the most abundant in the studied wood oil, have shown strong to moderate antioxidant activity (Ruberto and Baratta 2000;Thirugnanasampandan et al 2008), so the exhibited antioxidant activity could suggest to be attributed mainly to them.…”

Chemical composition, antioxidant, anti-elastase, and anti-inflammatory activities of Illicium anisatum essential oil

“…Considering the different groups of chemical compounds present in IAE, it is most likely that the biological activity is attributable to a synergism between components. Eucalyptol and kaurene have been reported to have significant anti-oxidant activity (11)(12)(13).…”

Cholinesterase-inhibitory diterpenoids and chemical constituents from aerial parts of Caryopteris mongolica

“…In assay methods, the AChE from electric eel, which is not a mammal, has often been used for screening AChE inhibitors because it is similar to the vertebrate nerve and muscle AChE [17]. Thus, in order to estimate the cholinesterase-inhibitory activities of the components isolated (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), including diterpenoids and diterpenoid glycosides, inhibition activities against AChE from human erythrocytes, AChE from electric eel, and BChE from horse serum were evaluated.…”

“…In this study, a diterpenoid diglucoside (1) and four flavone glycosides (2)(3)(4)(5) together with 15 known compounds (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), including two abietane-type diterpenoids (14,15) were obtained as the constituents of aerial parts of C. mongolica collected in Töv Province, Mongolia (Fig. 1).…”

“…1). In the last decade, various groups have reported Lamiaceae diterpenoids which have anticholinesterase activities, such as clerodane-type diterpenoids and tetracyclic diterpenoids from Ballota limbata [5], cis-clerodane-type tricyclic diterpenoids from Otostegia limbata [6], ent-kaurane diterpenoids from Isodon wightii and Sideritis arguta and congesta [7][8][9], cryptotanshinone from Salvia miltiorrhiza [10], kabdane-type diterpenoids from Leonurus heterophyllus [11], taxodion and ferruginol from Salvia stamines [12], and sclareol from Salvia chrysophylla [13]. These were mentioned as potent acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors because both AChE and BChE are targets of candidate phytochemicals for the treatment of neurodegenerative disorders such as Alzheimer's disease (AD) because of the cholinergic hypothesis [14].…”