Anti-tumor effects of Solanum nigrum L. extraction on C6 high-grade glioma

Li, Jiahui; Li, Song-Ya; Shen, Mingxue; Qiu, Runze; Fan, Hongwei; Li, Ying-bin

doi:10.1016/j.jep.2021.114034

Cited by 22 publications

(17 citation statements)

References 60 publications

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“…The antiproliferative activities of the crude organic solvent-based extracts of S. nigrum and isolated compounds were evaluated on tumor cell lines of liver (HepG2), ( Fekry et al, 2019 ) colon (HT29 and HCT-116), breast (MCF-7), ( Churiyah et al, 2020 ) and cervical (U14 and HeLa) ( Chothiphirat et al, 2019 , Moglad, 2019 ). Cytotoxic effects of a number of glycoalkaloids have also been considered on several cancer cell lines such as HepG2 ( Ahmad et al, 2017 , Ling et al, 2019 , Li et al, 2021 )…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Nawaz

Jamal

Arif

et al. 2021

Saudi Journal of Biological Sciences

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Plants have natural products which use to possess antiproliferative potential against many cancers. In the present study, six isolated fractions (ethyl acetate, petroleum ether, chloroform, n-butanol, ethanol and aqueous) from Solanum nigrum were evaluated for their cytotoxic effect on different cell lines. Hepatic carcinoma cell line (HepG2), cervical cancer cell line (HeLa) and baby hamster kidney (BHK) used as normal non-cancerous cells were evaluated for cytotoxicity against isolated fractions. Cell viability assay was performed to evaluate the cytotoxicity of all fractions on different cell lines followed by the lactate dehydrogenase and vascular endothelial growth factor assays of most active fraction among all screened for cytotoxic analysis. HPLC analysis of most active fractions against cytotoxicity was performed to check the biological activity of compounds. Results displayed the potent cytotoxic activity of ethyl acetate fraction of S. nigrum against HepG2 cells with IC 50 value of 7.89 μg/ml. Other fractions exhibited potent anticancer activity against HepG2 cells followed by HeLa cells. Fractions in our study showed no cytotoxicity in BHK cells. Cytotoxic activity observed in our current study exposed high antiproliferative potential and activity of ethyl acetate fraction against HepG2 cells. The results demonstrated that S. nigrum fractions exhibited anticancer activity against hepatic and cervical cancer cell lines with non-toxic effect in normal cells. These results reveal significant potential of S. nigrum for the therapeutic of cancers across the globe in future.

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Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Nawaz

Jamal

Arif

et al. 2021

Saudi Journal of Biological Sciences

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“…TMZ is a novel methylating agent that demonstrated activity against recurrent GBM and is ineffective due to drug resistance (Wu et al, 2021). TCMs were considered anti-GBM auxiliary drugs, such as Solanum nigrum L. (Li et al, 2021), Panax ginseng, licorice, Lycium barbarum, Salvia miltiorrhiza bunge, Coptis rhizoma, and Sophora flavescens (Wang et al, 2019). TCM is a helpful complementary and alternative medicine, however, there are few studies on TCM for GBM (Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…TCM is a helpful complementary and alternative medicine, however, there are few studies on TCM for GBM (Wang et al, 2019). The anti-GBM effects of TCM extract provided the new medium for the treatment of GBM (Li et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology and Inflammatory Microenvironment Strategy Approach to Finding the Potential Target of Siraitia grosvenorii (Luo Han Guo) for Glioblastoma

Li¹,

Bi²,

Zhang³

et al. 2021

Front. Genet.

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Background: Glioblastoma (GBM) is the most common and aggressive primary intracranial tumor of the central nervous system, and the prognosis of GBM remains a challenge using the standard methods of treatment—TMZ, radiation, and surgical resection. Traditional Chinese medicine (TCM) is a helpful complementary and alternative medicine. However, there are relatively few studies on TCM for GBM.Purpose: We aimed to find the connection between TCM and anti-GBM.Study design: Network pharmacology and inflammatory microenvironment strategy were used to predict Siraitia grosvenorii (Luo Han Guo) target for treating glioblastoma.Methods: We mainly used network pharmacology and bioinformatics.Results: CCL5 was significantly highly expressed in GBM with poor prognostics. Uni-cox and randomForest were used to determine that CCL5 was especially a biomarker in GBM. CCL5 was also the target for SG and TMZ. The active ingredient of Luo Han Guo — squalene and CCL5 —showed high binding efficiency. CCL5, a chemotactic ligand, was enriched and positively correlated in eosinophils. CCL5 was also the target of Luo Han Guo, and its effective active integrate compound –— squalene — might act on CCL5.Conclusion: SG might be a new complementary therapy of the same medicine and food, working on the target CCL5 and playing an anti-GBM effect. CCL5 might affect the immune microenvironment of GBM.

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“…However, the side effects of these drugs on the human body are becoming increasingly obvious and cannot be ignored. Doxorubicin can produce serious cardiac toxicity [ 15 ], and temozolomide can cause blood, skin, eyes, and gastrointestinal reactions [ 16 ]. Therefore, it is still urgent to explore new drugs for cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

“…Traditional Chinese medicine (TCM) has a history of thousands of years and is widely used in oncology [ 16 ]. About 30% of small molecules approved by the Food and Drug Administration between 1999 and 2008 are compounds or derivatives, both from natural products [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy

et al. 2022

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Cancers have always been the most difficult to fight, the treatment of cancer is still not considered. Thus, exploring new anticancer drugs is still imminent. Traditional Chinese medicine has played an important role in the treatment of cancer. Polyphenol oxidase (PPO) extracted from Edible mushroom has many related reports on its characteristics, but its role in cancer treatment is still unclear. This study aims to investigate the effects of PPO extracted from Edible mushroom on the proliferation, migration, invasion, and apoptosis of cancer cells in vitro and explore the therapeutic effects of PPO on tumors in vivo. A cell counting kit-8 (CCK8) assay was used to detect the effect of PPO on the proliferation of cancer cells. The effect of PPO on cancer cell migration ability was detected by scratch test. The effect of PPO on the invasion ability of cancer cells was detected by a transwell assay. The effect of PPO on the apoptosis of cancer cells was detected by flow cytometry. Female BALB/c mice (18–25 g, 6–8 weeks) were used for in vivo experiments. The experiments were divided into control group, model group, low-dose group (25 mg/kg), and high-dose group (50 mg/kg). In vitro, PPO extracted from Edible mushroom significantly inhibited the proliferation, migration, and invasion capability of breast cancer cell 4T1, lung cancer cell A549, and prostate cancer cell C4-2, and significantly promoted the apoptosis of 4T1, A549, and C4-2. In vivo experiments showed PPO inhibitory effect on tumor growth. Collectively, the edible fungus extract PPO could play an effective role in treating various cancers, and it may potentially be a promising agent for treating cancers.

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Anti-tumor effects of Solanum nigrum L. extraction on C6 high-grade glioma

Cited by 22 publications

References 60 publications

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Network Pharmacology and Inflammatory Microenvironment Strategy Approach to Finding the Potential Target of Siraitia grosvenorii (Luo Han Guo) for Glioblastoma

Polyphenol Oxidase as a Promising Alternative Therapeutic Agent for Cancer Therapy

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