Anti-tumor Effects of Cationic Hybrid Liposomes against Colon Carcinoma along with Apoptosis &lt;i&gt;in Vitro&lt;/i&gt;

Hino, Motoki; Ichihara, Hideaki; Matsumoto, Yōko; Ueoka, Ryuichi

doi:10.1248/bpb.b12-00633

Cited by 10 publications

(14 citation statements)

References 17 publications

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“…13) In addition, we elucidated the remarkably inhibitory effects on the growth of human colon cancer (HCT116) cells along with apoptosis by cationic HL including cationic lipid that targeted negatively charged characteristics of tumor cell membranes in vitro. 14) However, therapeutic effects of cationic HL in vivo have not yet been examined.…”

Section: )mentioning

confidence: 99%

“…Cationic HL were prepared by sonication (VS-N300; VELVO, Tokyo, Japan) of a mixture containing DMPC (purity>99%; NOF Co., Ltd., Tokyo, Japan), micellar molecules: C 12 (EO) 21 (Nikko Chemicals Co., Ltd., Tokyo, Japan) and 2C 14 ECl (DC-6-14; Sogo Pharmaceutical Co., Ltd. Tokyo, Japan) in 5% glucose solution at 45°C with 300 W, followed by filtration with a 0.20 µm filter.…”

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

“…HCT116 cells (5.0×10 6 cells) were intrasplenically transplanted into the mice. 16 21 , 65.7 mg/kg/d for 2C 14 ECl) were intravenously administered once each day for 14 d. Safety of 136 mg/kg/d for DMPC in chronic toxicity test using mice and rats has been reported. [17][18][19] After 14 d, the livers were isolated from the mouse models, fixed in 10% formalin solution, embedded in paraffin and sectioned at 5 µm of thickness.…”

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

“…HCT116 cells (5 21 , 65.7 mg/kg/d for 2C 14 ECl) were intravenously administered once each day for 14 d. The liver was removed from anaesthetized mice after the treatment with DMPC liposome, HL, and cationic HL and fixed in 10% formalin solution. The paraffin-embedded sections were made, and the microscopic observations of solid tumors were performed on the basis of TUNEL assay according to the conventional method.…”

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

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Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Ichihara

Hino

Ueoka

et al. 2014

Biological & Pharmaceutical Bulletin

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Therapeutic effects of cationic hybrid liposomes (HL) composed of 87 mol% dimyristoyl-phosphatidylcholine (DMPC), 5 mol% polyoxyethylene (21) dodecyl ether (C 12 (EO) 21 ) and 8 mol% O,O′-ditetradecanoyl-N-(α-trimethyl-ammonioacetyl) diethanolamine chloride (2C 14 ECl) on the metastasis of human colon carcinoma (HCT116) cells were examined in vivo. Cationic HL having a hydrodynamic diameter less than 150 nm were preserved for one month. Therapeutic effects were obtained in the hepatic metastasis mouse models of HCT116 cells after the intravenous injection of cationic HL. The histological analysis indicated the induction of apoptosis in the liver section of the hepatic metastasis mouse models treated with cationic HL in vivo. Therapeutic effects of cationic HL without any drugs on the hepatic metastasis were revealed for the first time on the basis of histological analyses in vivo.Key words hybrid liposome; hepetic metastasis; colon carcinoma; apoptosis; chemotherapy Colorectal cancer (CRC) is the third most common cancer in the world with more than 600000 deaths every year. 1)About 40% of patients with CRC develop metastases. Because the venous drainage of the colon is through the portal vein, which goes directly to the liver, more than 70% of the CRC metastases are located in the hepatic tissue. Metastatic diffusion results in a very poor prognosis with a median survival of about two years in treated patients. However, long term survival is possible in the 15% of patients that can benefit of hepatic metastasis surgery, usually after induction chemotherapy.2) Induction chemotherapy is a first-line treatment for cancer in which a patient is given doses of chemotherapy first. These doses may be high, with the goal of attempting to quickly attack the cancer, and after induction chemotherapy, additional treatment options such as surgical resection can be selected.3) While the chemotherapy drugs kill tumor cells, they also damage normal cells, causing severe side-effects such as gastrointestinal dysfunction and bone marrow toxicity. Therefore, chemotherapy that is effective for the metastasis of cancers without any side-effects is required.On the other hand, we have produced hybrid liposomes (HL) which can be prepared by just the sonication of vesicular and micellar molecules in a buffer solution. 4,5) HL are free from any contamination with organic solvents and remain stable for longer periods. The physical properties of these liposomes such as size, membrane fluidity, phase transition temperature, and hydrophobicity can be controlled by changing the constituents and compositional ratios of the HL. In the course of our study for HL, the following interesting results have been obtained. (a) Stereochemical control of the enantioselective hydrolysis of amino acid esters could be established by temperature regulation and changing the composition of the HL. 14) However, therapeutic effects of cationic HL in vivo have not yet been examined.In this study, we investigated the therapeutic effects of three-component cationic ...

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Section: )mentioning

confidence: 99%

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

Section: Preparation Of Hybrid Liposomesmentioning

confidence: 99%

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Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Ichihara

Hino

Ueoka

et al. 2014

Biological & Pharmaceutical Bulletin

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“…54 Hybrid liposomes composed of L-a-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(n)dodecyl ether (C 12 (EO) n ) were able to inhibit the proliferation of and induce apoptosis in human CRC cells in vitro. [55][56][57] Intravenous administration of DMPC/ C 12 (EO) n or DMPC/docosahexaenoic acid hybrid liposomes into a liver metastases mouse model of xenografted human CRC exhibited significant therapeutic effects. [58][59][60] Hybrid liposomes preferentially accumulated into cancer cells and triggered cancer cell apoptosis which resulted in the inhibition of hepatic malignant transformation and prolonged survival of CRC mice with liver metastasis.…”

Section: Hybrid Liposomesmentioning

confidence: 99%

Application of liposomes in drug development – focus on gastroenterological targets

Zhang¹,

Wang²,

Mao³

et al. 2013

IJN

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Over the past decade, liposomes became a focal point in developing drug delivery systems. New liposomes, with novel lipid molecules or conjugates, and new formulations opened possibilities for safely and efficiently treating many diseases including cancers. New types of liposomes can prolong circulation time or specifically deliver drugs to therapeutic targets. This article concentrates on current developments in liposome based drug delivery systems for treating diseases of the gastrointestinal tract. We will review different types and uses of liposomes in the development of therapeutics for gastrointestinal diseases including inflammatory bowel diseases and colorectal cancer.

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Mixed Hybrid Lipid/Polymer Vesicles as a Novel Membrane Platform

Schulz

Binder

2015

Macromol. Rapid Commun.

131

127

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Vesicles can be individually fabricated from naturally occurring lipid or synthetic block copolymer molecules via self-assembly in aqueous solutions; the blending of both vesicle-forming amphiphiles leads to the formation of hybrid membranes. Their final stabilities and lateral morphologies are strongly determined by the molar composition, size, and charge properties of the interacting components as well as by the lipid chain melting temperature. Upon merging the best properties of lipo- and polymersomal membranes, hybrid lipid/polymer vesicles represent a new scaffold for medical applications combining, e.g., combining the biocompatibility of liposomes with the high thermal and mechanical stability and functional variability of polymersomes within a single vesicle type. Up to now, several hybrid membrane systems and their corresponding vesicular morphologies have been studied, highlighting the attractive properties and features useful in selective delivery receptor scaffolding.

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Anti-tumor Effects of Cationic Hybrid Liposomes against Colon Carcinoma along with Apoptosis <i>in Vitro</i>

Cited by 10 publications

References 17 publications

Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Application of liposomes in drug development – focus on gastroenterological targets

Mixed Hybrid Lipid/Polymer Vesicles as a Novel Membrane Platform

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Anti-tumor Effects of Cationic Hybrid Liposomes against Colon Carcinoma along with Apoptosis <i>in Vitro</i>

Cited by 10 publications

References 17 publications

Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Therapeutic Effects of Cationic Hybrid Liposomes on the Hepatic Metastasis of Colon Carcinoma Along with Apoptosis <i>in Vivo</i>

Application of liposomes in drug development &ndash; focus on gastroenterological targets

Mixed Hybrid Lipid/Polymer Vesicles as a Novel Membrane Platform

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Product

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Application of liposomes in drug development – focus on gastroenterological targets