Anti-tumor effect of electrotherapy alone or in combination with interleukin-2 in mice with sarcoma and melanoma tumors

Serša, Gregor; Miklavčič, Damijan; Batista, Urška; Novaković, Srdjan; Bobanović, Fedja; Vodovnik, L.

doi:10.1097/00001813-199206000-00008

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…In contrast, the magnetically induced eddy currents in our study induced neither a pH change (data not shown) nor heat (2.66 Â 10 À10 8C/pulse), so they did not play a role in the anti-tumor effect observed in our present study. Several reports showed the functional activation of the immune system brought about by the application of electrical stimulation [Sersa et al, 1992;Chou et al, 1997;Miklavcic et al, 1997;Cabrales et al, 2001]. However, the effects of magnetic stimulation on immune systems were poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…Electrical stimulation such as direct current (DC) is known to induce various biological responses such as anti-tumor effects [Humphrey and Seal, 1959;David et al, 1985;Nordenstrom, 1989] and/or immunomodulatory effects, and a relationship between the antitumor effects was suggested [Sersa et al, 1992;Chou et al, 1997;Miklavcic et al, 1997;Cabrales et al, tumors and immune systems have not been well clarified [Jovanova-Nesic and Skokljev, 1990;Okada et al, 2002;Roman et al, 2002]. In our previous study, we reported on effects of pulsed magnetic stimulations on tumor growth in vivo and in vitro [Yamaguchi et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

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Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Yamaguchi

Ogiue-Ikeda

Sekino

et al. 2005

Bioelectromagnetics

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We investigated the effects of pulsed magnetic stimulation on tumor development processes and immune functions in mice. A circular coil (inner diameter = 15 mm, outer diameter = 75 mm) was used in the experiments. Stimulus conditions were pulse width = 238 micros, peak magnetic field = 0.25 T (at the center of the coil), frequency = 25 pulses/s, 1,000 pulses/sample/day and magnetically induced eddy currents in mice = 0.79-1.54 A/m(2). In an animal study, B16-BL6 melanoma model mice were exposed to the pulsed magnetic stimulation for 16 days from the day of injection of cancer cells. A tumor growth study revealed a significant tumor weight decrease in the stimulated group (54% of the sham group). In a cellular study, B16-BL6 cells were also exposed to the magnetic field (1,000 pulses/sample, and eddy currents at the bottom of the dish = 2.36-2.90 A/m(2)); however, the magnetically induced eddy currents had no effect on cell viabilities. Cytokine production in mouse spleens was measured to analyze the immunomodulatory effect after the pulsed magnetic stimulation. tumor necrosis factor (TNF-alpha) production in mouse spleens was significantly activated after the exposure of the stimulus condition described above. These results showed the first evidence of the anti-tumor effect and immunomodulatory effects brought about by the application of repetitive magnetic stimulation and also suggested the possible relationship between anti-tumor effects and the increase of TNF-alpha levels caused by pulsed magnetic stimulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Yamaguchi

Ogiue-Ikeda

Sekino

et al. 2005

Bioelectromagnetics

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“…When used in combination with certain drugs, the same type of electrotherapy potentiates the effectiveness of these compounds (8,9). With tumor necrosis factor-a (TNF-a), DC electrotherapy even effected the permanent removal of solid tumors in mice (10).…”

Section: Introductionmentioning

confidence: 99%

Blood Perfusion in a Murine Fibrosarcoma Tumor Model After Direct Current Electrotherapy a Study with⁸⁶Rb Extraction Technique

Jarm

Ĉmazˆar

Serša

et al. 1998

Electro- and Magnetobiology

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Electrotherapy with low-level direct current (DC) can induce antitumor effects in various tumor models. Applied in combination with certain anticancer drugs, it can significantly increase their effectiveness. It has been suggested that the demonstrated effects of electrotherapy arise from its modification of tumor blood flow. The effect of such treatment on blood perfusion of solid subcutaneous Sa-1 fibrosarcoma tumors in A/J mice was investigated with a &rubidium extraction technique. Following electrotherapy, the relative tissue perfusion of tumors was decreased by more than 50%. Three days after treatment, partial reperfusion of tumors occurred. The dynamics of the perfusion changes induced by electrotherapy are in agreement with tumor growth dynamics following this procedure. The effect of electrotherapy on the blood supply of tumors may be the major mechanism of antitumor action in our model. Electrotherapy could be useful as an adjuvant local procedure to other treatment modalities that require a hypoxic environment for their effectiveness.Address correseondence to: Toma; Jarm, University of Ljubljana, Faculty of Electrical Engineering, Triaska 25, SI-lo00 Ljubljana, Slovenia. Fax: +386 (0)61 1264-658.

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“…The best ECT effect was achieved by using a multi-array electrode. 69 The other interesting work of this group, mentioned in a previous Section, [51][52][53][54][55][56] uses ECT in combination with bleomycin or immunomodulators such as tumor necrosis factor (TNF-), interferon-(IFN-), interleukin-2 (IL-2) etc., with much beneficial effects.…”

Section: Electrochemical Treatment (Ect) Ofmentioning

confidence: 99%

Phenomenology and Mechanisms of Electrochemical Treatment (ECT) of Tumors

Vijh

Modern Aspects of Electrochemistry

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Anti-tumor effect of electrotherapy alone or in combination with interleukin-2 in mice with sarcoma and melanoma tumors

Cited by 24 publications

References 12 publications

Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Blood Perfusion in a Murine Fibrosarcoma Tumor Model After Direct Current Electrotherapy a Study with⁸⁶Rb Extraction Technique

Phenomenology and Mechanisms of Electrochemical Treatment (ECT) of Tumors

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Anti-tumor effect of electrotherapy alone or in combination with interleukin-2 in mice with sarcoma and melanoma tumors

Cited by 24 publications

References 12 publications

Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Effects of pulsed magnetic stimulation on tumor development and immune functions in mice

Blood Perfusion in a Murine Fibrosarcoma Tumor Model After Direct Current Electrotherapy a Study with86Rb Extraction Technique

Phenomenology and Mechanisms of Electrochemical Treatment (ECT) of Tumors

Contact Info

Product

Resources

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Blood Perfusion in a Murine Fibrosarcoma Tumor Model After Direct Current Electrotherapy a Study with⁸⁶Rb Extraction Technique