Anti-tumor and immunomodulatory activity of selenium (Se)-polysaccharide from Se-enriched Grifola frondosa

Mao, Guanghua; Ren, Yi; Li, Qian; Wu, Hui-Yu; Dun, Jin; Zhao, Ting; Xu, Cai-Quan; Zhang, Denghong; Jia, Qingdong; Bai, Yan-Peng; Yang, Liuqing; Wu, Xiangyang

doi:10.1016/j.ijbiomac.2015.10.083

Cited by 64 publications

(25 citation statements)

References 27 publications

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“…Similar result was reported that selenized pectic polysaccharide (isolated from Codonopsis pilosula) exhibited a linear random coil with the Se content of 478.17 μg/g (Chen et al, 2014b). Mao et al reported a globular conformation with random coil structure of Se-GP11 which was extracted and purified from Se-enriched Grifola frondosa (Mao et al, 2016).…”

Section: Solution Conformation Of Seaspssupporting

confidence: 78%

“…Selenium-polysaccharides, including functionalized selenium with polysaccharides, natural Se polysaccharides extracted from plants, bio-enrichment method or their chemically synthesized derivatives, are drawing the attention of researchers recently. The bio-activities of Se-polysaccharides are involved in cancer-therapeutic benefit in vitro/vivo (Wang et al, 2013;Shang et al, 2009;Yang 4 et al, 2012;Wu et al, 2013;Chen et al, 2008), immunoregulation (Mao et al, 2016;Gao et al, 2016), anti-fatigue (Chi et al, 2015), antibacterial (Lü, Gao, Shan & Lin, 2014), antioxidant and hypolipidemic activity (Mao et al, 2014;Liu et al, 2013). However, the selenium content of natural Se-polysaccharides is very low even in Se-enriched area.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

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It has been reported in our previous work that selenized Artemisia sphaerocephala polysaccharides (SeASPs) with the Se content range of 168-1703μg/g were synthesized by using Na2SeO3/HNO3/BaCl2 system. In the present work, the solution property of SeASP was studied by using size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS). A decrease in df values indicated that SeASPs with different conformational features that were highly dependent on MW. SeASPs exhibited a more rigid conformation (df value of 1.29-1.52) in low molecular weight range (MW of 1.026-1.426×10(4)g/mol) and compact spherical conformation in high molecular weight range (MW of 2.268-4.363×10(4)g/mol). It could be due to the degradation of polysaccharide chains in HNO3, which was supported in monosaccharide composition analysis. Congo red (CR) spectrophotometric method and atomic force microscopy (AFM) results also confirmed the conformational transition and the evidence on the shape of the rigid chains. In vitro anti-tumor assays, SeASP2 displayed greater anti-proliferative effects against three tumor cell lines (hepatocellular carcinoma HepG-2 cells, lung adenocarcinom A549 cells and cervical squamous carcinoma Hela cells) in a dose-dependent manner. This suggested that selenylation could significantly enhance the anti-tumor activities of polysaccharide derivatives in vitro.

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Section: Solution Conformation Of Seaspssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Wang

Bao

et al. 2016

Carbohydrate Polymers

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“…Similar result was shown by Mao et al that Se-enriched Grifola frondosa polysaccharide with a low Se content of 0.5981 μg/g exhibited no anti-tumor activity against HepG-2 cells in vitro[6]. It could be postulate that Se content is a key factor to influence the anti-tumor activities of selenized polysaccharides.…”

supporting

confidence: 83%

“…interleukin-2 (IL-2) levels in serum are observed. The results reveal that Se-GP11 exhibits the anti-tumor activity through improving immunomodulation activities of the tumor bearing mice [6]. Moreover, the biological activities of seleno-polysaccharides are related to antibacterial [7], antioxidant and hypolipidemic activity [8][9], anti-fatigue [10] and immuno-enhancement [11][12] In recent years, the use of microwave heating in chemical reactions has attracted a considerable amount of attention, owing to its advantages such as dramatically reduce processing times, increase product yields and enhance product purity or material properties compared to conventionally processed experiments [16][17].…”

Section: Introductionmentioning

confidence: 93%

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Wang

Yang

Bao

et al. 2017

International Journal of Biological Macromolecules

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In this work, selenylation of Artemisia sphaerocephala polysaccharide (SeASP) was studied by using HSeO/HNO/BaCl reaction system in microwave field. SeASP exhibited the Se content range of 111-264μg/g with high yields (72.1-94.9%). C NMR results indicated that the weak C-6 substitution was occurred. The decrease (from 7.348×10g/mol to 1.736-4.667×10g/mol) in weight average molecular mass (M) of SeASP was observed in size exclusion chromatography combined with multi angle laser light scattering (SEC-MALLS) analysis. SeASP exhibited a more rigid solution conformation which might be due to the degradation of polysaccharide chains in acidic reaction reagent. This was also supported by atomic force microscopy (AFM) result that SeASP showed short chains and island-like topography. In anti-tumor activity assays, SeASP exhibited the inhibition rates of 32.381% and 39.776% against human non-small cell lung cancer cell line (H1650) at the concentration of 100 and 200μg/mL, respectively. The relatively weak inhibition effect of SeASP was not related to cell apoptosis and cell cycle arrest, suggesting Se content might be a key factor to influence the anti-tumor activities of selenized polysaccharides in vitro.

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“…Most diseases of the human body, such as anemia, coronary heart disease, diabetes, and cancer, are related to a lack of selenium [13, 14]. Research has shown that organic selenium is more effective and safer than inorganic selenium as a dietary supplement [15] and that the biological activity of selenium polysaccharide is markedly higher than that of selenium or polysaccharide alone [16].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the fermentation process of Cordyceps sobolifera Se-CEPS and its anti-tumor activity in vivo

Yang

Zhang

2016

J Biol Eng

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BackgroundCordyceps sobolifera (C. sobolifera) isolated from cicadae was used as the starting fungus to produce selenium-enriched C. sobolifera extracellular polysaccharide (Se-CEPS). An orthogonal experimental design based on a single-factor experiment was used to optimize the C. sobolifera fermentation conditions, including the potato juice, peptone, and KH2PO4 concentrations. Ultraviolet (UV) and infrared (IR) analyses of CEPS and Se-CEPS were conducted, as well as an in vivo anti-tumor analysis.ResultsUnder optimal conditions (i.e., 40 potato juice, 0.4 KH2PO4, and 0.5 % peptone), the fermentation yield of Se-CEPS was 5.64 g/L. UV and IR spectra showed that Se-CEPS contained a characteristic absorption peak of a selenite Se = O double bond, demonstrating the successful preparation of Se-CEPS. Activity tests showed that Se-CEPS improved the immune organ index, serum cytokine content, and CD8+ and CD4+ T lymphocyte ratio in colon cancer CT26 tumor-bearing mice, thereby inhibiting tumor growth. When combined with 5-FU, Se-CEPS reduced the toxicity and enhanced the function of 5-FU.ConclusionThe result of these experiments indicated that orthogonal experimental design is a promising method for the optimization of Se-CEPS production, and the Se-CEPS from C. sobolifera can improve the anti-tumor capacity of mice.Electronic supplementary materialThe online version of this article (doi:10.1186/s13036-016-0029-0) contains supplementary material, which is available to authorized users.

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Anti-tumor and immunomodulatory activity of selenium (Se)-polysaccharide from Se-enriched Grifola frondosa

Cited by 64 publications

References 27 publications

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Synthesis of selenium-containing Artemisia sphaerocephala polysaccharides: Solution conformation and anti-tumor activities in vitro

Microwave-assisted synthesis, structure and anti-tumor activity of selenized Artemisia sphaerocephala polysaccharide

Optimization of the fermentation process of Cordyceps sobolifera Se-CEPS and its anti-tumor activity in vivo

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