Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

-The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.Index terms: Agaricus blazei, bioprospecting, fresh mushroom yield, polysaccharide-protein complex. Termos para indexação: Agaricus blazei, bioprospecção, produção de cogumelos frescos, complexo polissacarídeo-proteínas. Bactérias cultiváveis promotoras do crescimento do cogumelo e seu impacto sobre a produtividade de Agaricus blazei

Section: Methodsmentioning

confidence: 99%

Cultivable mushroom growth-promoting bacteria and their impact on Agaricus blazei productivity

Chu

Hameed

et al. 2013

Pesq. agropec. bras.

“…The products derived from the fruiting bodies of this genus can promote biological responses favouring cancer treatment in humans and have been used as antitumourigenic drugs (saRangi et al, 2006). These edible mushrooms are known to be Se accumulators.…”

mentioning

confidence: 99%

Selenium uptake and associated anti-oxidant properties inPleurotus fossulatuscultivated on wheat straw from seleniferous fields

Bhatia¹,

Bansal²,

Prakash³

et al. 2014

Acta Alimentaria

The present study was carried out to examine the antioxidant activity in oyster mushroom cultivated on selenium (Se) rich substrate. Pleurotus fossulatus was cultivated on Se-rich wheat straw collected from the seleniferous belt of Punjab (India) and its potential to accumulate Se from substrate was examined. Using different assay systems the modulations in the anti-oxidant profile of Se enriched mushroom was studied in comparison to the mushrooms cultivated on normal straw. The oyster mushrooms were observed to potentially mobilize Se from Se-rich substrates to fruiting bodies, resulting in significantly high uptake (37.2±0.6 mg g -1 ) as compared to control (3.57±0.53 mg g -1 ). The antioxidant activity, as determined by various assays, such as reducing power, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, and metal chelating activity, was higher in the experimental mushrooms when compared to control. The results obtained demonstrate that Se-fortified mushrooms through cultivation on straw containing organic forms of Se can be considered as natural and effective dietary supplements of organic Se for humans. The present study proposes the use of Se-rich agricultural residues as substrates for mushroom cultivation for human and livestock supplementation.

“…Polysaccharide-protein complex content was determined according to Sarangi et al (2006). Lyophilized PSPC was used to determine the polysaccharide content, using the phenol-sulfuric acid method (Sarangi et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

“…Lyophilized PSPC was used to determine the polysaccharide content, using the phenol-sulfuric acid method (Sarangi et al, 2006). All standards were purchased from Sigma Chemical Co., St. Louis, MO, USA.…”

Section: Methodsmentioning

confidence: 99%

Beneficial bacterial strains on Agaricus blazei cultivation

Chu

2012

Pesq. agropec. bras.

-The objective of this work was to evaluate the effects of six bacterial strains isolated from Agaricus blazei (ABM) on its cultivation. The six strains were characterized as to their effects on the productivity, polysaccharide-protein complex (PSPC), and polysaccharide contents of ABM cultured on sterilized casing soils. Three isolates enhanced ABM mycelium growth. Inoculation of Arthrobacter sp. or Exiguobacterium sp. on sterile peat casing soil resulted in 64% increase in ABM mushroom total fresh matter yield compared to the uninoculated control. Inoculation of Exiguobacterium sp., Microbacterium esteraromaticum or Pseudomonas resinovorans on sterilized loamy casing soil resulted in 62, 95, and 59% increase in ABM mushroom total fresh matter yield, respectively. The PSPC content in ABM increased 7 to 10% in casing soil inoculated with five of the six isolates compared to the uninoculated control. Exiguobacterium sp. inoculated on casing soil resulted in a mushroom-polysaccharide content 15% higher than the control. Moreover, inoculation of five of the six isolates on the casing soil reduced the harvesting time from 10 to 27 days. The evaluated beneficial microbes improve the yield, PSPC, and polysaccharide contents, besides reducing the harvesting time in ABM culture.Index terms: Agaricus blazei, beneficial microbe, casing soil, mushroom productivity, polysaccharide-protein complex, probiotics. Estirpes bacterianas benéficas ao cultivo de Agaricus blazeiResumo -O objetivo deste trabalho foi avaliar os efeitos de seis estirpes bacterianas isoladas de Agaricus blazei (ABM) sobre o seu cultivo. As seis estirpes foram caracterizadas quanto a seus efeitos sobre a produtividade, o conteúdo do complexo polissacarídeo-proteína (CPP) e o conteúdo de polissacarídeos, em ABM cultivado em solos esterilizados. Três isolados aumentaram o crescimento micelial de ABM. A inoculação de Arthrobacter sp. ou Exiguobacterium sp. em cobertura de turfa estéril resultou em aumento de 64% na produção total de cogumelos, em comparação ao controle sem inoculação. A inoculação de Exiguobacterium sp., Microbacterium esteraromaticum ou Pseudomonas resinovorans em solo argiloso esterilizado resultou em 62, 95, e 59% de aumento na produção total de cogumelos, respectivamente. O conteúdo de CPP em ABM aumentou de 7 a 10% em solo com inoculação de cinco dos seis isolados, em comparação ao controle sem inoculação. A inoculação de Exiguobacterium sp. em terra de cobertura resultou em conteúdo de polissacarídeo nos cogumelos 15% superior ao do controle. Além disso, a inoculação de cinco dos seis isolados na terra de cobertura reduziu o tempo de colheita de 10 a 27 dias. Os microrganismos benéficos avaliados melhoram a produção e os conteúdos de CPP e de polissacarídeos, além de reduzir o tempo de colheita no cultivo de ABM.Termos para indexação: Agaricus blazei, microrganismo benéfico, terra de cobertura, produtividade de cogumelos, complexo polissacarídeo-proteína, probiótico.