Anti-Tumor Activity Of Four Ayurvedic Herbs In Dalton Lymphoma Ascites Bearing Mice And Their Short-Term <i>In Vitro</i> Cytotoxicity On DLA- Cell-Line

Abstract

The anti-tumor activity and chemopreventive potential of four Ayurvedic herbs viz. Curcuma longa L., Ocimum sanctum L., Tinospora cordifolia (Wild) Miers ex Hook.f & Thomas and Zizyphus mauritiana Lam. were evaluated using Dalton Lymphoma ascites (DLA) tumor model in Swiss Albino mice. The outcome was assessed using survival time, peritoneal ascitic fluid (Tumor volume) and hematological indices as parameters. Animals were divided into five groups (n = 6) viz. one DLA control and four Herb + DLA treated groups. All the four herb + DLA groups were pre-treated with respective herbs for 7 days and hematological indices were measured for entire five groups. On day-8 animals were inoculated with 1x10 6 DLA cells i.p., and Herb + DLA groups were continued with oral herbal treatment for 21-days. Hematological parameters and tumor volume were assessed to find the effects of herbs. Short term in vitro cytotoxicity was determined by Trypan Blue exclusion method and LDH leakage assay using different concentrations of herbal extracts and 5-FU as a positive control and IC 50 for each herbal extract and 5-FU were determined. Oral administration of crude herb increased the survival time and decreased the peritoneal ascitic fluid content significantly. Hb, RBCs and total WBC which were altered by DLA inoculation were restored significantly by all the herbs except O. sanctum. All the four herbs showed in vitro cytotoxic activity against DLA cell-line. Moreover inter group comparison of all the four herbs for anti-tumor activity showed efficacy in the following order-T. cordifolia > Z. mauritiana ≥ C. longa > O. sanctum respectively.

Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells

“…Have been reported, various parts from O. sanctum Linn. provide several advantages as an anti-inflammatory [12], antiallergic [13], antioxidant [14,15], radioprotective [16], and anticarcinogenic [17]; however until today, there is only restricted research explore how is the mechanism of O. sanctum Linn. act as neuroprotective [11,18].…”

Aqueous Ethanolic Extract of Tinospora cordifolia as a Potential Candidate for Differentiation Based Therapy of Glioblastomas

“…The plant extract and epoxy cleordane isolated from this plant have been shown to possess chemoprotective potential [29–31]. Several recent studies have reported that various extracts of Tinospora cordifolia plant possess bioactive components which inhibit cellular proliferation in various in vitro models and also show antineoplastic [32], antitumor [33–35], anti-angiogenesis [36,37] and anti metastatic activity in various in vivo models [35,37,38]. …”

Evaluation of native and exotic Brazilian plants for anticancer activity

“…Among them are Baccharis dracunculifolia [20], Bidens pilosa [21], Chelidonium majus [22], Chenopodium ambrosioides [23], Citrus limon [24], Coffea arabica [25], Curcuma longa [26], C. zedoaria [27], Hypericum perforatum [28], Leonurus sibiricus [29], Malpighia glabra [30], Malva sylvestris [31], Maytenus ilicifolia [32], Mikania hirsutissima [33], Nicotiana tabacum [34], Oenothera biennis [35], Origanum majorana [36], Petiveria alliacea [37], Phyllanthus tenellus [38], Piper tuberculatum [39], Schinus molle [40], S. terebinthifolia [41], Senna obtusifolia [42], Solanum cernuum [43], Stevia rebaudiana [44], Taraxacum officinale [45], Tetradenia riparia [46], Tropaeolum majus [46], Urtica dioica [47], and Vernonia polyanthes [48] (Table 1). Similarly, the carcinogenesis preventive extract of Hibiscus rosa-sinensis [49] also afforded low to moderate values.…”