Anti-Tuberculosis Drugs and Human Polymorphonuclear Leukocyte Functions

Kielland, Live Gran; Vage, Richard André; Eide, Geir Egil; Sørnes, Steinar; Naess, A

doi:10.1159/000330442

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…Most studies have focused on the effects of antibiotics on immune cellular functions [22,23,24]. In this study, changes in the immunomodulatory gene expression in LPS-stimulated PMNs and cellular function were identified.…”

Section: Discussionmentioning

confidence: 99%

Effects of Erythromycin and Rifampicin on Immunomodulatory Gene Expression and Cellular Function in Human Polymorphonuclear Leukocytes

Ubagai

Kikuchi-Ueda

et al. 2013

Chemotherapy

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Background: We investigated the effects of two antibiotics, erythromycin and rifampicin, on the immunomodulatory gene expression and cellular function of human polymorphonuclear leukocytes (PMNs). Methods: We used real-time quantitative PCR to examine the expression of immunomodulatory genes. The production of reactive oxygen species (ROS) was determined by fluorescence-activated cell sorting. PMN chemotaxis was analyzed using a KK chemotaxis chamber. Results: Stimulation of PMNs with lipopolysaccharide (LPS) resulted in increases in the mRNA levels of immunomodulatory genes. Rifampicin significantly inhibited the overexpression of TLR2, TLR4, CD14 and IL8Rs. However, erythromycin suppressed only the upregulation of TLR2 and TNFA. Neither antibiotic had an effect on the production of ROS. Rifampicin significantly inhibited PMN chemotaxis, but erythromycin had no effect. Conclusions: Erythromycin and rifampicin may play anti-inflammatory roles by affecting the expression levels of immunomodulatory genes or the chemotaxis of PMNs.

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Section: Discussionmentioning

confidence: 99%

Effects of Erythromycin and Rifampicin on Immunomodulatory Gene Expression and Cellular Function in Human Polymorphonuclear Leukocytes

Ubagai

Kikuchi-Ueda

et al. 2013

Chemotherapy

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“…Discussion: Neutrophil function tests are an integral component of a clinical immunology laboratory and play a decisive role in the diagnosis of several congenital and acquired phagocyte function disorders (1). In addition, these tests are also being used in research to study the immunomodulatory activities of drugs, synthesized compounds and natural products (7)(8)(9).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Three Different Techniques for Isolation of Neutrophils From Blood and Their Utility in Performing Nitroblue Tetrazolium Test

Pattan¹,

Bhat²,

Pattar³

et al. 2020

Int. J. Res. Granthaalayah

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Background & Objectives: Assays for neutrophils constitute an important component of screening tests in clinical immunology. There are no standard protocols for performing many of these tests and procedures vary from one laboratory to another. In addition, normal ranges for these assays in healthy Indian population have not been defined. Hence, an attempt is made to evaluate and present a simple technique for WBC isolation and NBT test. Methods: The study involved participation of 30 healthy adult volunteers. Ten of blood sample collected from each subject was subjected to three different procedures for isolation of WBCs - Ficoll-Hypaque gradient, dextran sedimentation and gelatin sedimentation methods. Cells isolated from these procedures were then used to perform NBT test. Smears were prepared, stained with Giemsa and results were expressed as % of stimulated and unstimulated cells. Results: The mean cell yield from both dextran and gelatin methods was comparable (2921.67cells vs 2806.67cells/cu mm). The cell yield from Ficoll-Hypaque method was much lower (1408.33 cells/cu mm). In NBT test, the mean readings of stimulated (61%) and unstimulated cells (18%) were almost similar in all three procedures of cell isolation. Conclusions: Comparison of procedures show that gelatin and dextran sedimentation methods yield high amount of relatively purified WBCs. The efficacy of cells isolated from all three procedures in NBT test was almost similar. The range of stimulated and unstimualted cells in the subjects were within expected levels. Gelatin sedimentation is economical, easy to perform and can be adopted to any clinical laboratory for WBC isolation.

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Comparison of four methods for the isolation of murine blood neutrophils with respect to the release of reactive oxygen and nitrogen species and the expression of immunological receptors

et al. 2013

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Anti-Tuberculosis Drugs and Human Polymorphonuclear Leukocyte Functions

Cited by 6 publications

References 42 publications

Effects of Erythromycin and Rifampicin on Immunomodulatory Gene Expression and Cellular Function in Human Polymorphonuclear Leukocytes

Effects of Erythromycin and Rifampicin on Immunomodulatory Gene Expression and Cellular Function in Human Polymorphonuclear Leukocytes

Comparison of Three Different Techniques for Isolation of Neutrophils From Blood and Their Utility in Performing Nitroblue Tetrazolium Test

Comparison of four methods for the isolation of murine blood neutrophils with respect to the release of reactive oxygen and nitrogen species and the expression of immunological receptors

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