Anti-Trypanosoma cruzi activity of 10 medicinal plants used in northeast Mexico

Molina-Garza, Zinnia Judith; Bazaldúa-Rodríguez, Aldo Fabio; Quintanilla-Licea, Ramiro; Galaviz‐Silva, Lucio

doi:10.1016/j.actatropica.2014.04.006

Cited by 46 publications

(30 citation statements)

References 61 publications

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“…Thus, numerous studies perform screening experiments with epimastigotes and/or trypomastigotes further approach the selected active compounds in intracellular amastigotes (e.g. Pizzolatti et al., 2003, Molina-Garza et al., 2014, Legarda-Ceballos et al., 2015, Olivera et al., 2015) or in vivo .…”

Section: Discussionmentioning

confidence: 99%

Effects of a novel β–lapachone derivative on Trypanosoma cruzi : Parasite death involving apoptosis, autophagy and necrosis

Anjos

Alves

Goncalves

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

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Natural products comprise valuable sources for new antiparasitic drugs. Here we tested the effects of a novel β–lapachone derivative on Trypanosoma cruzi parasite survival and proliferation and used microscopy and cytometry techniques to approach the mechanism(s) underlying parasite death. The selectivity index determination indicate that the compound trypanocidal activity was over ten-fold more cytotoxic to epimastigotes than to macrophages or splenocytes. Scanning electron microscopy analysis revealed that the R72 β–lapachone derivative affected the T. cruzi morphology and surface topography. General plasma membrane waving and blebbing particularly on the cytostome region were observed in the R72-treated parasites. Transmission electron microscopy observations confirmed the surface damage at the cytostome opening vicinity. We also observed ultrastructural evidence of the autophagic mechanism termed macroautophagy. Some of the autophagosomes involved large portions of the parasite cytoplasm and their fusion/confluence may lead to necrotic parasite death. The remarkably enhanced frequency of autophagy triggering was confirmed by quantitating monodansylcadaverine labeling. Some cells displayed evidence of chromatin pycnosis and nuclear fragmentation were detected. This latter phenomenon was also indicated by DAPI staining of R72-treated cells. The apoptotis induction was suggested to take place in circa one-third of the parasites assessed by annexin V labeling measured by flow cytometry. TUNEL staining corroborated the apoptosis induction. Propidium iodide labeling indicate that at least 10% of the R72-treated parasites suffered necrosis within 24 h. The present data indicate that the β–lapachone derivative R72 selectively triggers T. cruzi cell death, involving both apoptosis and autophagy-induced necrosis.

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Section: Discussionmentioning

confidence: 99%

Effects of a novel β–lapachone derivative on Trypanosoma cruzi : Parasite death involving apoptosis, autophagy and necrosis

Anjos

Alves

Goncalves

et al. 2016

International Journal for Parasitology: Drugs and Drug Resistan

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“…The extracts were found effective against E. histolytica; the IC 50 was found to be 7 at a dose of 12 µg/ml and slightly moderate toxic to G. lamblia. Besides an in vitro trypanocidal activity of M. vulgare along with other plants used in traditional Mexican medicine for the treatment of parasitic infections was also investigated [120]. The results concluded that methanol extract of M. vulgare (aerial parts) was found effective against Trypanosoma cruzi epimastigotes and exhibited the highest trypanocidal activity, percentage inhibition was found in between 88% and 100% at a concentration of 150 µg/ml.…”

Section: Antiprotozoal Activitymentioning

confidence: 83%

Marrubium vulgare L.: A review on phytochemical and pharmacological aspects

Lodhi¹,

Vadnere²,

Sharma³

et al. 2017

J Intercult Ethnopharmacol

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Marrubium vulgare L. (family: Lamiaceae), also known as the white horehound, is widely used as an herbal remedy for chronic coughs and colds. It is used in various disorders related to skin, liver, gastric, heart, and immune system. This review abridges phytochemical, pharmacological studies, and medicinal uses of M. vulgare and provides scientific proof for various ethnobotanical claims to identify gaps, which will give impulsion for novel research on M. vulgare based herbal medicines. This review summarizes selected scientific evidence on phytochemistry and pharmacological properties of M. vulgare over the past 48 years . Works related to M. vulgare was reviewed from various sources such as books, internet source, i.e., Google Search engine, PubMed, and Science Direct, and chemical abstract. The exhaustive literature was studied, and critical analysis was performed according to their phytochemical and pharmacological properties. Phytochemical investigations on different parts of M. vulgare have been reported the presence of flavonoids, steroids, terpenoids, tannins, saponins, and volatile oils (0.05%). The aerial parts contain marrubiin, together with ursolic acid and choline. Pharmacological activities such as antinociceptive, antispasmodic, antihypertensive, antidiabetic, gastroprotective, anti-inflammatory, antimicrobial, anticancer, antioxidant, and antihepatotoxic activity have been reported. M. vulgare has therapeutic potential in the treatment of inflammatory conditions, liver disorders, pain, cardiovascular, gastric, and diabetic conditions. Aerial parts of M. vulgare is a good source of labdane type diterpene especially marrubiin which is present in high concentrations. However, further scientific studies are needed to explore clinical efficacy, toxicity and to explore the therapeutic effect of major secondary metabolites such as diterpenes, phenylpropanoid, and phenylethanoid glycosides of M. vulgare.

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“…El extracto de S. molle ha sido utilizado ampliamente como repelente de insectos, CUADRO 3 Compuestos identificados en extractos de Haematoxylum brasiletto mediante pruebas coloridas incluyendo para Triatoma infestans, vector de la enfermedad de Chagas, identificándose hasta 65 compuestos con actividad repelente, como son el limoneno, eucaliptol y terpenos; sin embargo, su uso antiparasitario se ha restringido solo al extracto completo (Ferrero, Gonzá-lez, & Chopa, 2006;Abdel-Sattar et al, 2010;Molina-Garza et al, 2014). Del extracto de M. vulgare se han identificado cinco compuestos de una fracción polar con actividad anti-inflamatoria no esteroidea: el ácido cafeoil-L-máli-co, verbascosido, forsitosida, arenariosido y ballotetrosido; de los cuales sólo los dos primeros compuestos se han reportado con actividad antibiótica importante (Sahpaz, Garbacki, Tits, & Bailleul, 2002).…”

Section: Discussionunclassified

“…Se utilizaron diversas porciones de los ejemplares, como la parte aérea, tallos y hojas (Molina-Garza et al, 2014). Se pesaron 50 g del material vegetal molido y se depositó en un cartucho de celulosa (Cartucho Whatman 33 x 80 mm) para la extracción con el aparato Soxhlet con 250 mL de metanol absoluto (J. T. Baker) como solvente de extracción, se colocó a reflujo a 60 ºC por 40 h. Posteriormente, se filtró el extracto con papel Whatman N°1 y se concentró con el rotavapor Buchi R-205.…”

Section: Preparación De Los Extractosunclassified

“…C. tortuosa Liebm, 1853) (Simaroubaceae) en la disentería amebiana (Monzote, Alarcón, & Setzer, 2012), Lippia graveolens (Kunth, 1818) (Verbenaceae) en los casos de desórdenes gastrointestinales y giardiasis (Monzote et al, 2012); Persea americana (Mill, 1768) (Lauracea), la cual se usa como tratamiento de infecciones micóticas (Wang et al, 2004) y como nematicida (Dang et al, 2010) Ruta chalepensis (Linnaeus, 1767) (Rutaceae), que se considera un buen antihelmíntico y espasmolítico (Güna-ydin & Savci, 2005), Eryngium heterophyllum (Engelm, 1848) (Apiaceae), como tratamiento para diarrea, dolor de estómago fiebre y colelitiasis (Camou-Guerrero, Reyes-García, Martínez-Ramos, & Casas, 2008), Haematoxylum brasiletto (Karst, 1862) (Fabaceae) para tratar infecciones bacterianas (Rosas-Piñón et al, 2012), Marrubium vulgare (Linnaeus, 1753) (Lamiaceae), usado como hipotensivo (Bardai, Lyoussi, Wibo, & Morel, 2001) y Schinus molle (Linnaeus, 1753) (Anacardiaceae) que es un tratamiento antiespasmódico e hipotensivo (Yueqin et al, 2003). En el estudio previo, Molina-Garza et al (2014), concluyen que los extractos de las últimas cuatro plantas exhibieron la mayor actividad e inhibieron el crecimiento entre el 88 y 100 %; incluso, cabe mencionar que se ha experimentado con un amplio espectro de productos naturales contra T. cruzi, pero muy pocos han sido útiles a una concentración inhibitoria recomendada del 50 % (CI 50 ) de 10 µg/mL, en referencia a la CI 50 para nifurtimox y para benidazol, la cual debe ser menor a los 3 µg/mL (Werner & Zulantay, 2011), por lo cual fueron seleccionados para continuar con este proyecto.…”

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Actividad contra Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) de extractos metanólicos de plantas de uso medicinal en México

Pérez-Treviño

Galaviz

Iracheta-Villarreal

et al. 2017

RBT

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Activity against Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) of methanolic extracts of medicinal use plants in Mexico. American trypanosomiasis is a potentially lethal disease caused by the hemoflagellate protozoan Trypanosoma cruzi. This neglected disease affects from 6 to 7 million people worldwide. Currently there are only two medicines to treat this disease: beznidazol and nifurtimox, both effective if they are administrated in the acute phase of infection, although their effectiveness fades away in the chronic phase; it also induces significant side effects. The aim of this study is to screen the trypanocidal activity of methanolic extracts from Hematoxilum brasiletto, Marrubium vulgare, Schinus molle, and Cympongon citratus, against T. cruzi epimastigotes, followed by the chromatographic separation, and identification of active compounds of the best candidate by colored chemical reactions; furthermore, it was also determined their cytotoxic effect in human lymphocytes and the brine shrimp Artemia salina. The extract of H. brasiletto showed the highest anti-T. cruzi activity with a inhibitory concentration (IC 50 ) of 543 µg/mL; in descending order, it was followed by M. vulgare (IC 50 = 647 µg/mL), S. molle (IC = 827 µg/mL) and finally, C. citratus (IC = 1 210 µg/mL). The chromatographic fraction Fr22 from H. brasiletto showed the best anti-T. cruzi effectivity (IC 50 = 0.238 mg/mL), when compared to the other fraction or the whole extract, with no cytotoxic effect against human lymphocytes or A. salina. The active compounds were identified as tannins, quinones, flavonoids and sesquiterpenlactones. In conclusion, active compounds against T. cruzi were identified for the first time in H. brasiletto, with non-cytotoxic effects. The H. brasiletto extract, according to our results, could be used as an alternative treatment for the Chagas disease; however, additional studies will be necessary to test their activity and doses in a murine model, with the complete identification of the active compounds, on which we are investigating. Rev. Biol. Trop. 65 (4) La tripanosomiasis americana es una enfermedad potencialmente mortal causada por el protozoario Trypanosoma cruzi (Chagas, 1909). Las zonas endémicas abarcan 21 países de América Latina, donde el parásito se transmite principalmente por vía vectorial (Calvo, 2015). La enfermedad presenta dos fases; el inicio de la infección es la fase aguda, y permanece hasta dos meses con parasitemia, fiebre y malestar. La etapa crónica, que se desarrolla después de algunos años en el 25-35 % de las personas infectadas, se caracteriza por no presentar parasitemia; en esta fase, los parásitos se alojan y reproducen en nidos de amastigotes de órganos y sistema nervioso periférico, de los cuales un 30 % desarrolla desórdenes cardíacos y un 10 %, trastornos nerviosos y digestivos como el megacolon o megaesófago (Martino, 2012). Actualmente, los movimientos migratorios la han diseminado desde las regiones endé-micas hasta Canadá y varios países europeos (Slot, Ho...

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Anti-Trypanosoma cruzi activity of 10 medicinal plants used in northeast Mexico

Cited by 46 publications

References 61 publications

Effects of a novel β–lapachone derivative on Trypanosoma cruzi : Parasite death involving apoptosis, autophagy and necrosis

Effects of a novel β–lapachone derivative on Trypanosoma cruzi : Parasite death involving apoptosis, autophagy and necrosis

Marrubium vulgare L.: A review on phytochemical and pharmacological aspects

Actividad contra Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) de extractos metanólicos de plantas de uso medicinal en México

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