Anti-thrombotic effects of α-linolenic acid isolated from Zanthoxylum bungeanum Maxim seeds

Abstract

BackgroundThe current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo.MethodsThe seeds were crushed and subsequently subjected to saponification, acid hydrolysis, gradient freezing, urea inclusion and complexation of silver nitrate to obtain the unsaturated fatty acids. The chemical characteristics of isolated ALA were validated by 1HNMR, 13CNMR and mass spectrometry, and then the anti-thrombotic effect of ALA and its mixture with linoleic acid (1:1) were evaluated in the following experiments.ResultsThe alpha-linolenic acid was isolated and purified from Jiaomu through our newly established methods. ALA and its mixture with linoleic acid can prolong the hemorrhage and coagulation time as well as enhanced the survival rate of mice subjected to collagen-adrenaline induced thrombosis. In addition, the thrombosis on A-V bypass and platelet aggregation of rats will be reduced after treated with ALA or its mixture, and the expression level of Akt and PI3K protein decreased 26% and 31%, respectively.ConclusionsWe designed and optimized a very simple and high-yield procedure to isolate ALA and linoleic acid mixture from seeds of Zanthoxylum bungeanum Maxim and demonstrated that such mixture can obtain a good anti-thrombotic effect through the modulation of PI3K/Akt signaling.

Antimelanogenic Effects of Raphanus sativus L. var. niger Roots on α‐MSH Stimulated B16F10 Melanoma Cells

“…This is known to inhibit melanogenesis by downregulation of MITF via ERK activation. [13][14][15][16][17][18] For instance, compound 1 showed six signals in 13 C NMR spectrum corresponding to one thione (δ 194.9), two olefinic (δ 139.2 and 136.1), two methylene (δ 47.1 and 27.2), and one methylthio (δ 17.5) carbons. The melanin contents and cell viabilities with varying concentrations (10-40 μg/mL) were also examined for Hex and EtOAc fractions ( Figure 1).…”

Chemometrics-Assisted Identification of Anti-Inflammatory Compounds from the Green Alga Klebsormidium flaccidum var. zivo

“…Reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled with a single quadrupole mass spectrometer (qMS) and a multi-channel UV detector was utilized to generate chemical profiles of the 21 Figure 1C) [12][13][14][15][16]. A rapid structural identification or prediction of these compounds was conducted as follows.…”

“…A rapid structural identification or prediction of these compounds was conducted as follows. Figure 1C) [12][13][14][15][16]. A rapid structural identification or prediction of these compounds was conducted as follows.…”

The effect of Zanthoxylum bungeanum maxim extract on crow's feet: A double‐blind, split‐face trial

“…In addition, ZBM is used in traditional Chinese medicine for the treatment of abdominal pain, dyspepsia, parasitosis, and eczema. Recent studies have revealed the many pharmacological effects of ZBM, including antibacterial, anti‐inflammatory, antioxidant, neuroprotective and anti‐ wrinkles (Artaria, Maramaldi, Bonfigli, Rigano, & Appendino, ; Tang et al, ; Yang et al, ; Zhang, Luo, et al, ).…”