Antimelanogenic Effects of Raphanus sativus L. var. niger Roots on α‐MSH Stimulated B16F10 Melanoma Cells

Abstract

This study was conducted to identify the antimelanogenic components from Raphanus sativus L. var. niger (black radish). Phytochemical study with the ethanol extract of black radish roots resulted in the isolation of six compounds: 3-(E)-(methylthio)methylene-2-pyrrolidinethione (1), ascorbigen (2), β-sitosterol (3), palmitic acid (4), α-linolenic acid (5), and 1-linoleonyl glycerol (6). Among the isolates, the thione 1 efficiently inhibited melanin synthesis in a concentration-dependent manner in α-MSH stimulated B16F10 melanoma cells. Western blotting analysis for the key melanogenic enzymes showed that compound 1 decreased tyrosinase and tyrosinase-related protein-2 expressions. These results demonstrated that the black radish extract including pyrrolidinethione 1 could be useful as antimelanogenic agents in cosmetic or pharmaceutical applications.

Separation and anti‐inflammatory evaluation of phytochemical constituents from Pleurospermum candollei (Apiaceae) by high‐speed countercurrent chromatography with continuous sample load

“…13 C‐NMR (MeOH‐ d 4 , 100 MHz) δ C : 181.0 (COOH, C‐1), 132.7 (CH, C‐9), 131.0 (CH, C‐10), 129.1 (CH, C‐13), 129.1 (CH, C‐12), 128.8 (CH, C‐15), 128.2 (CH, C‐16), 36.2 (CH 2 , C‐2), 30.6 (CH 2 , C‐4), 30.3 (CH 2 , C‐7), 30.3 (CH 2 , C‐5), 30.2 (CH 2 , C‐6), 28.1 (CH 2 , C‐3), 26.5 (CH 2 , C‐8), 26.5 (CH 2 , C‐11), 26.3 (CH 2 , C‐14), 21.4 (CH 2 , C‐17), 14.6 (CH 3 , C‐18). The spectral information was found in comparison to the literature [29] and the compound was named α‐linolenic acid with the molecular formula C 18 H 30 O 2 .…”