Anti-thrombotic effect of strawberries

Abstract

Because of the high mortality, prevention of arterial thrombotic disease has top priority in developed countries. As inappropriate diet is known to predispose to acute thrombotic events, regular intake of an anti-thrombotic diet may offer a convenient and effective method of prevention. As part of a systematic investigation into the anti-thrombotic effect of fruits and vegetables, strawberry varieties were tested in this study. An in vitro platelet function test (haemostatometry) was used for screening strawberry filtrates. Those that showed significant antiplatelet effect were further assessed with a laser-induced thrombosis test in mice. Measurement of flow-mediated vasodilation in the femoral artery of mice reflected the effect on the vascular endothelium. Correlation between the effects on platelet reactivity in vitro and the antioxidant activity (hypoxanthine/xanthine oxidase test) or phenolic compound content was assessed. Strawberry varieties KYSt-4 (Nohime), KYSt-11 (Kurume IH-1) and KYSt-17 (Kurume 58) showed significant antiplatelet activity both in vitro and, after oral administration, in vivo. Both KYSt-11 and KYSt-17, but not KYSt-4, significantly reduced flow-mediated vasodilation; that is, caused endothelial dysfunction. Antiplatelet activities were heat stable. Significant correlation was found between antiplatelet and antioxidant activities (P=0.049, R=0.23) or total phenolic compounds (P=0.0096, R=0.36). Of the tested strawberry varieties, KYSt-4, KYSt-11 and KYSt-17 showed significant anti-thrombotic effect. The dual mechanism of the effect may involve a direct inhibition of both platelet function and antioxidant activities.

Platelet Aggregation Inhibitors from Aerial Parts of Ruta Chalepensis Grown in Jordan

“…Garlic, onion, ginger, strawberries and tomatoes are well known and well studied representatives in this aspect (Rahman and Billington, 2000;Goldman et al 1996;Chrubasik et al 2005;Dutta Roy et al 2001). Compounds responsible for the inhibition of platelet aggregation have been determined and reported, such as sulphurous compounds in garlic and onion, gingerol and zingiberine in ginger and phenolics in strawberries (Naemura et al 2005). Several medicinal plants with diverse active constituents such as alkaloids, fl avonoids, tannins and coumarins have shown promising results in inhibition of the platelet aggregation (Afi fi and Aburjai, 2005;El Haouari et al 2006;Mekhfi et al 2004;Mekhfi et al 2006;Sheen et al 1996;Teng et al 1992).…”

Mechanism of the Antithrombotic Effect of Dietary Diacylglycerol in Atherogenic Mice

“…Earlier we have developed a sensitive and reproducible thrombosis model using heliumneon laser irradiation to form a thrombus in the carotid artery of mice deficient in ApoE and LDLR [4] . This method enabled us to assess the effect of diets on thrombus formation [10,13,18,[28][29][30] . Using this test, we could demonstrate an antithrombotic effect of DAG [10] .…”

“…This method has been described in details elsewhere [15][16][17][18] . Animals were anesthetized with sodium pentobarbital (65 mg/ kg, i.m.)…”

Chemopreventive Potential of Select Herbal Teas and Spices on Azoxymethane-Induced Aberrant Crypt Foci in Fisher 344 Male Rats

“…There have been numerous health benefits of strawberry fruit, including increased serum antioxidant capacity [8], and anti-thrombotic effects [9].…”