Platelet Aggregation Inhibitors from Aerial Parts of Ruta Chalepensis Grown in Jordan

Abstract

Abstract:From the aerial parts of Ruta chalepensis L., grown in Jordan, two furanocoumarins (bergapten and chalepensin), one fl avonoid glycoside (rutin) as well as several minor compounds have been isolated. The structural elucidation of these compounds was established based on spectral data (UV, IR, MS, 1 H-NMR and 13 C-NMR). In Jordan, R. chalepensis is recommended for the treatment of rheumatism, mental disorders and menstrual problems. Fresh and dried leaves are used as fl avoring agent in food and beverages. Antiplatelet activities of the crude methanolic and ethylacetate extracts in addition to the three isolated major compounds were measured by the aggrometric method according to Beretz and Casenave. Optical aggregometer connected to dual channel recorder was used for measuring aggregation. Both, ethylacetate and methanol extracts inhibited ADP-induced platelet aggregation (ADP-IA) of human blood. However, only ethylacetate extract was able to induce 50% inhibition of collagen-induced platelet aggregation (Co-IA) platelet rich plasma. Bergapten was more active against ADP-IA compared to chalepensin while the latter was more active against Co-IA compared to bergapten.

Fumigant Toxicity of Rue Essential Oil, Ruta Graveolens L. on Tetranychus Urticae Koch (Acari: Tetranychidae)

“…The genus Ruta features Shrubby plants that are native to the Mediterranean region and are represented by 40 species in the world [18], which were used as traditional medicine in many countries to treat a variety of diseases such as rheumatism, neuralgia, menstrual bleeding, fever, arthritis, hepatic diseases, antifertility, and gastrointestinal disorders [19]. Also, recent studies [20][21][22], revealed that rue species have analgesic, antipyretic, anti-inflammatory, antifungal, emmenagogue, insect repellent, molluscicidal, nematicidal, antimicrobial, anthelmintic, sedative, and antiplatelet properties. R. graveolens is a small aromatic shrub cultivate in Egypt and known for its medicinal aromatic properties, since ancient times [23].…”

Selected Wild Plants Ethanol Extracts Bioactivity on the Coagulation Cascade

“…In addition, Ruta chalepensis is recorded as possible apportion factor among pregnant women, eliciting its prospect effect on the circulatory system. This coincided with its use in traditional medicine in menstrual problems as was proved to have antiplatelet activities [28]. The remaining examined plant species were chosen due to their other recorded bioactivities [22].…”

Microwave-assisted Extraction and RP-HPLC Quantification of Bergapten from Pithecellobium dulce

“…Bergapten is a fumaro-coumarin, which has acquired a place in the treatment of psoriasis and other dermatoses [1] . It also showed platelet aggregation inhibitory activity [2] .…”