Anti-rheumatoid activity of ethanolic extract of Sesamum indicum seed extract in Freund's complete adjuvant induced arthritis in Wistar albino rats

Abstract

Sesamum indicum, one of the first recorded plants used for its seeds, is reported to have analgesic, antioxidant, anticancer, anti-obesity as well as hepato and nephro protective activities. The current study evaluated the effects of two doses (400 and 800 mg/kg) of ethanolic extract of S. indicum seeds in Freund's complete adjuvant induced arthritis in rats in comparison with diclofenac and methotrexate by the changes produced in body weight, body temperature, paw volume and spontaneous activity, hemoglobin, erythrocyte sedimentation rate, total white blood cells, red blood cells, Interleukin-6 and Tumor necrosis factor-α as well as joint changes in X-ray and histological changes in joint tissue. Unlike the untreated group, the groups treated with S. indicum showed significant decrease in paw volume, body weight, white blood cell count, erythrocyte sedimentation rate, Interleukin-6 and Tumor necrosis factor-α and an increase in body weight, spontaneous activity, hemoglobin level, and red blood cell count. Histopathological examination showed gross reduction in synovial inflammation and cartilage damage. X-ray revealed significant improvement in joint space. The effect of ethanolic extract of S. indicum was found to be equivalent to methotrexate and greater than diclofenac.

Effectiveness of Sesame Seeds Cookies (Sesamum Indicum Seeds) Combination of Iron In Increasing Hemoglobine Levels Of Adolescents

“…Teenage girls who took Fe tablets combined with 91 mg sesame seed cookies had higher hemoglobin levels 1,600 than girls who were only given 1,000 Fe tablets with p = 0,000. This is in line with research conducted on albino rats where sesame seed extract induced an increase in hemoglobin and red blood cell levels (Ruckmani et al, 2018). This is because sesame seeds contain a variety of essential nutrients to increase hemoglobin levels, including flavonoids that can ward off free radicals.…”

Anti-Inflammatory and Anticancer Properties of Bioactive Compounds from Sesamum indicum L.—A Review

“…Correspondingly, BSSEE with concentrations of 1 ppm and 2 ppm promotes the activation of Nrf2 and improves the levels of MAPKs and NF-кB. In another study of BSSEE, Freund's complete adjuvant (FCA)-induced arthritis rat model was used [116]. The study examined the effect of BSSEE on the inflammatory cytokines (IL-6 and TNF-α).…”

Clinical Assessment of Anti-inflammatory Activity of 940 Nanometer Low Level laser Therapy on Carrageenan Induced Arthritis in Temporomandibular Joint in Wistar Albino Rats

“…Induction of arthritis was made using the most common method used to evaluate the effects of different types of treatment protocols on inflammatory arthritis which is induction of arthritis using carrageenan solution injected directly into TMJ, carrageenan is polysaccharide obtained from sea weed, it have potent pro-inflammatory effect in animal joint when injected into the joint space 11 , previous studies had proof the carrageenan effectiveness in arthritis induction in TMJ of rats 19,20,24,25 . Pilot study revealed massive edema into TMJ area and decrease amount of food intake by rats suffering from arthritis comparing to normal rats and apparently that decrease in food consumption caused these rats look thinner, thus we made an attempt to evaluate these clinical findings caused by inducted TMJ arthritis and the effect of treatment protocol on head circumference changes caused by edema and weight changes caused by decrease food consumption due to loss proper function of inflamed TMJ.According to our knowledge there was no similar articles available that used head circumference and/or weight changes as clinical parameters for evaluating treatment protocol in induced arthritis, although several authors were report relations between similar parameters and pan-arthritis or joint arthritis (other than TMJ) in human studies as well as animal experimental studies including studies on rats, Vierboom et al, reported significant relation between weight changes and several diseases including arthritis in observational study in human 26 , Leech et al, found relation between lower extremities arthritis with weight and growth in human study 27 , Borb‫י‬ly et al, mentioned significant weight loss in mouse after induction of experimental autoimmunity arthritis 28 ,in the same way Hasan et al, reported similar weight loss in induced paw arthritis inSprague-Dawley rats using complete Freund's adjuvant 29 , identical weight loss secondary to induced arthritis were reported by several authors in Westar rats induced arthritis 21,22,30,32 , also there were several articles used body weight as parameter to assess TMJ function as secondary outcome depending on the fact that limited mouth opening would interfere with normal feeding such as the study of Angelo et al, and Richmond who did randomized clinical trial on Black Merino sheep temporomandibular joint andMiyamoto et al, who used body weight of mice as valuable index for evaluation TMJ diseases 33,35 . As mentioned above no specific head circumference measurement as parameter to evaluate TMJ edema was previously reported, but many authors used similar way to assess edema in arthritic joints by measuring diameters or volume changes, it's good to mention some examples of these techniques; Uttra et al, used Vernier caliper to measure paw diameter to evaluate paw induced arthritis in rats 31 , others relied on volume changes in paws to assess i n d u c e d a r t h r i t i s u s i n g d i s p l a c e m e n t plethysmometry 29,36,...…”

“…Each animal was weighted before the induction of arthritis and every next day till the end of experiment (eight times) to evaluate weight changes 21,22 , and percentage of weight changes were recorded for each animal.Circumferences of animal head were measured using measuring tape in a circle around the head just anteriorly to the ears in line over TMJs in both sides before the induction of arthritis and every next day till the end of experiment (eight times) to evaluate circumferences changes which represent edema in TMJ 23 and percentage of circumferences changes were recorded for each animal.…”