Anti-quorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa

Doğan, Şule; Gökalsın, Barış; Şenkardeş, İsmail; Doğan, Ahmet; Sesal, Nüzhet Cenk

doi:10.1016/j.jep.2019.02.020

Cited by 32 publications

(16 citation statements)

References 43 publications

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“…Hypericum L. (Hypericaceae) is comprised of approximately 500 species that can be found around the world. Various products from Hypericum species have been used as antidepressant, sedative, diuretic, antiphlogistic, analgesic, astringent, and antipyretic remedies in Europe, America, Africa, and Asia [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. One of the most intensively studied medicinal plants from this genus is H. perforatum L. or St. John’s wort, which is a perennial herb that his known for its beneficial pharmacological properties [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

“…For example, H. perforatum L. has been widely used in many countries in antibacterial, antiviral, anti-inflammatory, antinociceptive, or analgesic remedies [ 4 ]. Extracts from this herb have also been reported as a therapeutic remedy for burns, skin wounds, cuts, stomach aches, and ulcers [ 5 ]. In addition, H. perforatum extracts have also been reported to have anti-angiogenic, anti-fibroblastic, and antioxidant properties [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition and Immunomodulatory Activity of Hypericum perforatum Essential Oils

Schepetkin

Özek

et al. 2020

Biomolecules

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Hypericum L. (Hypericaceae) extracts have been used for their therapeutic effects; however, not much is known about the immunomodulatory activity of essential oils extracted from this plant. We isolated essential oils from the flowers and leaves of H. perforatum and analyzed their chemical composition and innate immunomodulatory activity. Analysis of flower (HEOFl) versus leaf (HEOLv) essential oils using gas chromatography–mass spectrometry revealed that HEOFl was comprised mainly of monoterpenes (52.8%), with an abundance of oxygenated monoterpenes, including cis-p-menth-3-en-1,2-diol (9.1%), α-terpineol (6.1%), terpinen-4-ol (7.4%), and limonen-4-ol (3.2%), whereas the sesquiterpenes were found in trace amounts. In contrast, HEOLv was primarily composed of sesquiterpenes (63.2%), including germacrene D (25.7%) and β-caryophyllene (9.5%). HEOLv also contained oxygenated monoterpenes, including terpinen-4-ol (2.6%), while monoterpene hydrocarbons were found in trace amounts. Both HEOFl and HEOLv inhibited neutrophil Ca2+ mobilization, chemotaxis, and reactive oxygen species (ROS) production, with HEOLv being much more active than HEOFl. Furthermore, the pure sesquiterpenes germacrene D, β-caryophyllene, and α-humulene also inhibited these neutrophil responses, suggesting that these compounds represented the active components of HEOLv. Although reverse pharmacophore mapping suggested that potential protein targets of germacrene D, β-caryophyllene, bicyclogermacrene, and α-humulene could be PIM1 and mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAK2), a kinase binding affinity assay did not support this finding, implying that other biological targets are involved. Our results provide a cellular and molecular basis to explain at least part of the beneficial immunotherapeutic properties of the H. perforatum essential oils.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Immunomodulatory Activity of Hypericum perforatum Essential Oils

Schepetkin

Özek

et al. 2020

Biomolecules

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“…This elegant regulatory strategy has attracted extensive attention to develop therapeutics against P. aeruginosa-related infections, particularly acute infections. A wide array of natural and synthetic compounds targeting QS systems have been developed and tested in vitro and in vivo (Starkey et al, 2014;Asfour, 2018;Dogan et al, 2019). For example, furanone C-30 (Hentzer et al, 2002), gingerol (Kim et al, 2015) and garlic extract (Rasmussen et al, 2005) have been reported to possess anti-QS activities.…”

Section: Discussionmentioning

confidence: 99%

Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen that can infect a wide variety of hosts including humans, plants, and animals. The production of virulence factors is the determinant of the infection paradigm and is under orchestrated regulation via cell-to-cell communication process called quorum sensing (QS). To disable QS circuits and prevent bacterial infections, a large battery of anti-QS agents, particularly from traditional Chinese medicine have been developed. Here, we used P. aeruginosa as a model microorganism to investigate the effect of traditional Chinese medicine Tanreqing (TRQ) formula on bacterial pathogenicity. Phenotypic analysis showed that TRQ treatment could completely inhibit the production of phenazine pyocyanin and moderately inhibit the production of virulence factors such as rhamnolipids, elastase, and alkaline protease. Further transcriptomic analyses revealed that TRQ treatment could significantly attenuate the expression of QS-regulated genes in P. aeruginosa and TRQ-treated P. aeruginosa regulon shared a large overlap with QS regulon. Component contribution to QS inhibition shed light on the indispensable role of all five components in TRQ formula. Further genetic analysis indicated that upstream regulators of QS systems, including two-component systems GacS/GacA and PprA/PprB, were both inhibited by TRQ treatment. Finally, our TRQ formula could efficiently protect Caenorhabditis elegans from killing by P. aeruginosa. Altogether, we have proved TRQ formula as an effective and specific agent to attenuate bacterial virulence and combat bacterial infections.

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“…Punicalagin, an active ingredient in the Chinese herbal medicine Punica granatum L., downregulates the expression of Serratia marcescens virulence-related genes and the expression of QS-related genes (sdiA and srgE), reducing the invasion of colon cells by S. marcescens [90]. Trans-cinnamaldehyde (CA) and salicylic acid (SA) in Cinnamomum cassia Presl also effectively downregulate both the las and rhl QS systems and significantly reduce virulence phenotypes at both the transcriptional and extracellular levels in P. aeruginosa PAO1 [91]. Zeaxanthin, an active constituent of Chinese herbal medicine Lycium barbarum L., reduces the expression of the virulence factors of Pseudomonas aeruginosa by QS [92].…”

Section: Regulation Of Natural Products On Bacterial Virulencementioning

confidence: 99%

Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing

Yang

Meng

et al. 2020

BioMed Research International

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Quorum sensing (QS) has emerged as a research hotspot in microbiology and medicine. QS is a regulatory cell communication system used by bacterial flora to signal to the external environment. QS influences bacterial growth, proliferation, biofilm formation, virulence factor production, antibiotic synthesis, and environmental adaptation. Through the QS system, natural products can regulate the growth of harmful bacteria and enhance the growth of beneficial bacteria, thereby improving human health. Herein, we review advances in the discovery of natural products that regulate bacterial QS systems.

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Anti-quorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa

Cited by 32 publications

References 43 publications

Chemical Composition and Immunomodulatory Activity of Hypericum perforatum Essential Oils

Chemical Composition and Immunomodulatory Activity of Hypericum perforatum Essential Oils

Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa

Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing

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