Anti-Proliferative and Anti-Biofilm Potentials of Bacteriocins Produced by Non-Pathogenic Enterococcus sp.

Molham, Fatma; Khairalla, Ahmed S.; Azmy, Ahmed F.; El-Gebaly, Eman; El-Gendy, Ahmed O.; AbdelGhani, Sameh

doi:10.1007/s12602-020-09711-1

Cited by 14 publications

(9 citation statements)

References 76 publications

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“…In our previous studies of inhibition of foodborne pathogens, we showed that L. brevis bacteriocin effectively inhibited the biofilm formation of Escherichia coli and Salmonella Typhimurium (Kim et al, 2019), and Pediococcus acidilactici bacteriocin displayed antibiofilm activities against S. Typhimurium, Pseudomonas aeruginosa, and Enterococcus faecalis (Lee et al, 2020;Seo and Kang, 2020;Yoon and Kang, 2020). Recent studies have also shown that bacteriocins of lactic acid bacteria possess antibiofilm activity against S. mutans (Conrads et al, 2019;Dufour et al, 2020;Molham et al, 2021). In accordance with the previous reports, the current study demonstrated that DB1 BLIS could be an effective antibiofilm agent to control S. mutans.…”

Section: Resultsmentioning

confidence: 95%

“…Typhimurium, Pseudomonas aeruginosa , and Enterococcus faecalis ( Lee et al, 2020 ; Seo and Kang, 2020 ; Yoon and Kang, 2020 ). Recent studies have also shown that bacteriocins of lactic acid bacteria possess antibiofilm activity against S. mutans ( Conrads et al, 2019 ; Dufour et al, 2020 ; Molham et al, 2021 ). In accordance with the previous reports, the current study demonstrated that DB1 BLIS could be an effective antibiofilm agent to control S. mutans .…”

Section: Resultsmentioning

confidence: 99%

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Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation

Kim¹,

Kim²,

Lee³

et al. 2022

Food Sci Anim Resour

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation

Kim¹,

Kim²,

Lee³

et al. 2022

Food Sci Anim Resour

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“…The cytotoxicity of the crude extracts of bioactive actinomycetes was assessed using the methyl thiazolyl tetrazolium (MTT) (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay as described earlier [24,25] with few modifications. The cancer cell lines, human hepatoma HepG2 cells, human breast cancer cell line MCF-7 and corresponding normal cell lines, namely, THLE2 and MCF-10A, were, respectively, purchased from the American Type Culture Collection (ATCC).…”

Section: Methodsmentioning

confidence: 99%

Anti-cancer and antimicrobial potential of five soil Streptomycetes : a metabolomics-based study

et al. 2022

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Lack of new anti-cancer and anti-infective agents directed the pharmaceutical research to natural products' discovery especially from actinomycetes as one of the major sources of bioactive compounds. Metabolomics- and dereplication-guided approach has been used successfully in chemical profiling of bioactive actinomycetes. We aimed to study the metabolomic profile of five bioactive actinomycetes to investigate the interesting metabolites responsible for their antimicrobial and anti-cancer activities. Three actinomycetes, namely, Streptomyces sp. SH8, SH10 and SH13, were found to exhibit broad spectrum of antimicrobial activities, whereas isolate SH4 showed the broadest antimicrobial activity against all tested strains. In addition, isolates SH8, SH10 and SH12 displayed potent cytotoxicity against the breast cancer cell line Michigan Cancer Foundation-7 (MCF-7), whereas isolates SH4 and SH12 exhibited potent anti-cancer activity against the hepatoma cell line hepatoma G2 (HepG2) compared with their weak inhibitory properties on the normal breast cells MCF-10A and normal liver cells transformed human liver epithelial-2 (THLE2), respectively. All bioactive isolates were molecularly identified as Streptomyces sp. via 16S rRNA gene sequencing. Our actinobacterial dereplication analysis revealed putative identification of several bioactive metabolites including tetracycline, oxytetracycline and a macrolide antibiotic, novamethymycin. Together, chemical profiling of bioactive Streptomycetes via dereplication and metabolomics helped in assigning their unique metabolites and predicting the bioactive compounds instigating their diverse bioactivities.

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“…Mueller-Hinton agar (Oxoid) was used, and the test was proceeding, as described ( 6 ). The plates were incubated at 37°C for 24 hr before measuring and recording zones of inhibition surrounding the discs as described ( 7 ).…”

Section: Methodsmentioning

confidence: 99%

Exploring clinically isolated Staphylococcus sp. bacteriocins revealed the production of amonabactin, micrococcin, and α-circulocin

Kassem¹,

Saafan²,

Bayomy³

et al. 2021

IJM

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Background and Objectives: Bacteriocins are considered alternative non-conventional antimicrobials produced by certain bacteria with activity against closely related species. The present study focuses on screening, characterization, and partial purification of bacteriocins produced by Staphylococcus sp. isolated from different clinical sources such as pus and blood. Materials and Methods: A total of 100 Staphylococcus isolates were screened for bacteriocin production using spot on lawn assay and agar diffusion method against five indicator bacteria. Bacteriocins from five selected highly active isolates were subjected to proteinase-K enzyme, different pH, and heating at different temperatures, and investigated the stabilities of their antimicrobials. Two selected isolates, MK65 and MK88, were molecularly identified by 16S rRNA gene sequencing, explored for the presence of 18 bacteriocin genes, and liquid chromatography-high resolution electrospray ionization mass spectrometry (LC-HRESIMS) was used to identify their different metabolites. Results: Twenty isolates exhibited inhibitory effect against at least one indicator bacteria. Micrococcus luteus ATCC 4698 showed the highest sensitivity to such bacteriocins. Proteinase K, acidic pH, and heating at 100°C triggered marked activity inhibition. However, amylase enzyme, alkaline pH, and heating at 80°C caused trivial effects. Four out of eighteen bacteriocin genes were detected using PCR. Fermentation, partial purification, and LC-HRESIMS of total protein extracts of two selected isolates, MK65 and MK88, revealed the production of different antimicrobial peptides. Conclusion: To the best of our knowledge, this is the first study to report the production of micrococcin and α-circulocin from Staphylococcus aureus MK65 and the production of amonabactin from Staphylococcus epidermidis MK88.

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Anti-Proliferative and Anti-Biofilm Potentials of Bacteriocins Produced by Non-Pathogenic Enterococcus sp.

Cited by 14 publications

References 76 publications

Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation

Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from Enterococcus faecium DB1 on Cariogenic Streptococcus mutans Biofilm Formation

Anti-cancer and antimicrobial potential of five soil Streptomycetes : a metabolomics-based study

Exploring clinically isolated Staphylococcus sp. bacteriocins revealed the production of amonabactin, micrococcin, and α-circulocin

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