Anti-proliferative activities of Centella asiatica extracts on human respiratory epithelial cells in vitro

Heikal, Mohd; Mariam, Siti; Ilham, Mohd; Fong, Mee; Latiff, Jalan Yaacob; Razak, Bandar Tun; Raja, Jalan; Dato, Taman; Razali, A. R.

doi:10.5897/jmpr12.660

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…The survived cells continued to proliferate thus showing an increase in cell number after day 4 till day 6 except in 1000 μg/ mL C.asiatica supplemented group. Our results also showed that the effect of C.asiatica against fibroblast proliferation was dosage dependent and this was in agreement with study by Mohd Heikal et al (2014).…”

Section: Discussionsupporting

confidence: 82%

“…Asiatic acid (AA), which is one of the major components of C.asiatica has been shown to possess antioxidant and antiinflammatory activities which prevents the infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) of cigarette smoke exposure mice (Lee et al 2016). However, the extract of C.asiatica has shown significant cytotoxicity on various human cells including airway epithelial cells above certain concentration (Mohd Heikal et al 2014;Sampson et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

Lokanathan¹

2017

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ABSTRAK Model tiga dimensi (3D) menyerupai ciri-ciri persekitaran tisu asli, justeru morfologi dan isyarat-isyarat sel daripada kultur 3D selalunya lebih menyerupai fisiologi asal berbanding sel kultur dua dimensi (2D ABSTRACTThree dimensional (3D) models mimic the features of native tissue environment. Thus, morphology and signalling of cells from 3D culture are often more physiological than routine two dimensional (2D) cell culture. It is also known that the cell-secreted products have paracrine effect on other cells growth. In this experimental study, we optimised the nasal fibroblast culture on a 3D cell culture system and studied the effects of secretome from the 3D culture (3DCM) onto fibroblast growth and cytoprotection. Nasal fibroblast was isolated from human nasal turbinates. The suitable microcarrier was selected by culturing the fibroblasts in passage 3 on various types PolyGEM™ polystyrene microcarriers. Then, the cells were cultured on selected microcarrier using a 3D culture system and the conditioned medium (CM) was collected. 3DCM were supplemented to fibroblasts to study for attachment, proliferation, and cytoprotective effects against cytotoxicity of Centella asiatica. Bicinchonic Acid Assay (BCA) was performed to quantify protein amount in CMs. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed for preliminary profiling and comparison of 2DCM and 3DCM protein profile. Our study showed that the 3DCM did not significantly enhance cell attachment and proliferation. The secretome of both 2DCM and 3DCM found to have significant cytoprotective effect onto nasal fibroblast against cytotoxicity of C.asiatica extract. 3DCM had higher protein concentration than 2DCM. SDS-PAGE showed three exclusive proteins in 3DCM and four exclusive proteins in 2DCM. Future study should be conducted on utility of nasal fibroblast secretome on cytoprotection against harmful agents in environment and cytotoxicity of natural products.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

Lokanathan¹

2017

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“…RECA exerted anti-proliferative effects on hWJMSCs in a dose-dependent manner, as at higher concentrations ≥1200 μg/ml, it significantly inhibited cell proliferation. Similar trend in the inhibition of cell growth was also reported on human cornea and respiratory epithelium cells upon supplementation of C. asiatica (L.) extract at higher concentrations [31, 32]. The inhibitory effects of C. asiatica (L.) might be due to the presence of madecassoside and asiaticoside in the C. asiatica (L.) extract, which inhibit the proliferation of keratinocytes [33].…”

Section: Discussionsupporting

confidence: 64%

The effects of Centella asiatica (L.) Urban on neural differentiation of human mesenchymal stem cells in vitro

Omar

Lokanathan

Razi

et al. 2019

BMC Complement Altern Med

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Background Centella asiatica (L.) Urban, known as Indian Pennywort, is a tropical medicinal plant from Apiaceae family native to Southeast Asian countries. It has been widely used as a nerve tonic in Ayuverdic medicine since ancient times. However, whether it can substitute for neurotrophic factors to induce human mesenchymal stem cell (hMSCs) differentiation into the neural lineage remains unknown. This study aimed to investigate the effect of a raw extract of C. asiatica (L.) (RECA) on the neural differentiation of hMSCs in vitro. Methods The hMSCs derived from human Wharton’s jelly umbilical cord (hWJMSCs; n = 6) were treated with RECA at different concentrations; 400, 800, 1200, 1600, 2000 and 2400 μg/ml. The cytotoxicity of RECA was evaluated via the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) and cell proliferation assays. The hWJMSCs were then induced to neural lineage for 9 days either with RECA alone or RECA in combination with neurotrophic factors (NF). Cell morphological changes were observed under an inverted microscope, while the expression of the neural markers S100β, p75 NGFR, MBP, GFAP and MOG was analyzed by quantitative polymerase chain reaction and immunocytochemistry. The cell cycle profile of differentiated and undifferentiated hWJMSCs was investigated through cell cycle analysis. Results RECA exerted effects on both proliferation and neural differentiation of hWJMSCs in a dose-dependent manner. RECA reduced the proliferation of hWJMSCs and was cytotoxic to cells above 1600 μg/ml, with IC 50 value, 1875 ± 55.67 μg/ml. In parallel with the reduction in cell viability, cell enlargement was also observed at the end of the induction. Cells treated with RECA alone had more obvious protein expression of the neural markers compared to the other groups. Meanwhile, gene expression of the aforementioned markers was detected at low levels across the experimental groups. The supplementation of hWJMSCs with RECA did not change the normal life cycle of the cells. Conclusions Although RECA reduced the proliferation of hWJMSCs, a low dose of RECA (400 μg/ml), alone or in combination of neurotrophic factors (NF + RECA 400 μg/ml), has the potential to differentiate hWJMSCs into Schwann cells and other neural lineage cells. Electronic supplementary material The online version of this article (10.1186/s12906-019-2581-x) contains supplementary material, which is available to authorized users.

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“…Centella asiatica is one of the most popular herbs that can be found abundantly in China, Japan, Italy, Sri Lanka, Iran, India, Madagascar, America, Australia, South Africa, Indonesia, and Malaysia [ 2 , 3 , 4 , 5 ]. It is a perennial herb in the Umbelliferae family and presents medicinal properties such as anti-inflammatory [ 6 , 7 , 8 ], anti-ulcer [ 9 ], antimicrobial [ 10 , 11 , 12 ], and memory-enhancing properties [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on Different Extraction Methods of Centella asiatica and Extracts Bioactive Compounds Effects on Antimicrobial Activities

Idris

Nadzir

2021

Antibiotics

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The interest of consumers in using products containing phytochemicals derived from plants is growing day by day due to the shift of consumers’ preferences from convenience to environmental sustainability. One plant utilized in many products is Centella asiatica, a herb commonly used in folk medicine, health supplements, and beauty products. Extraction of bioactive compounds from C. asiatica was performed using conventional methods and modern methods (e.g., microwave or ultrasound-assisted and subcritical water extraction). This review summarizes the variety of methods used to extract active compounds from C. asiatica, their influence on the bioactive compounds and antimicrobial activity in vitro and in vivo, and the safety and toxicology of C. asiatica extract.

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Anti-proliferative activities of Centella asiatica extracts on human respiratory epithelial cells in vitro

Cited by 7 publications

References 23 publications

Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

Effects of Secretome from Dynamic 3D Cell Culture System onto Growth and Cytoprotection of Nasal Fibroblast

The effects of Centella asiatica (L.) Urban on neural differentiation of human mesenchymal stem cells in vitro

Comparative Studies on Different Extraction Methods of Centella asiatica and Extracts Bioactive Compounds Effects on Antimicrobial Activities

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