Anti-poliovirus activity of<i>Baccharis dracunculifolia</i>and propolis by cell viability determination and real-time PCR

Búfalo, Michelle Cristiane; Figueiredo, A.; Sousa, João Paulo Barreto de; Candeias, J. A. N.; Bastos, Jairo K.; Sforcin, José Maurício

doi:10.1111/j.1365-2672.2009.04354.x

Cited by 95 publications

(63 citation statements)

References 21 publications

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“…This product has attracted researchers' interest in the last decade because of its numerous biological and pharmacological properties, such as antimicrobial (Sforcin et al, 2000;Freitas et al, 2006;Búfalo et al, 2009), antitumoral (Bazo et al, 2002), immunomodulatory (Sforcin, 2007), anti-inflammatory (Silva et al, 2012), antioxidant (Kumazawa et al, 2010), among others.…”

Section: Introductionmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Búfalo

Ferreira

Costa

et al. 2013

Journal of Ethnopharmacology

152

102

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Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages, Journal of Ethnopharmacology, http://dx.doi.org/ 10. 1016/j.jep.2013.06.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractEthnopharmacological relevance: Propolis is a bee product with numerous biological and pharmacological properties, such as immunomodulatory and anti-inflammatory activities.It has been used in folk medicine as a healthy drink and in food to improve health and prevent inflammatory diseases. However, little is known about its mechanism of action.Thus, the goal of this study was to verify the antioxidant activity and to explore the antiinflammatory properties of propolis by addressing its intracellular mechanism of action.Caffeic acid was investigated as a possible compound responsible for propolis action. Materials and Methods:The antioxidant properties of propolis and caffeic acid were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging method. To analyze the anti-inflammatory activity, Raw 264.7 macrophages were treated with different concentrations of propolis or caffeic acid, and nitric oxide (NO) production, a strong pro-inflammatory mediator, was evaluated by the Griess reaction. The concentrations of propolis and caffeic acid that inhibited NO production were evaluated on intracellular signaling pathways triggered during inflammation, namely p38 mitogenactivated protein kinase (MAPK), c-jun NH 2 -terminal kinase (JNK1/2), the transcription nuclear factor (NF)-NB and extracellular signal-regulated kinase (ERK1/2), through Western blot using specific antibodies. A possible effect of propolis on the cytotoxicity of hepatocytes was also evaluated, since this product can be used in human diets.Results: Caffeic acid showed a higher antioxidant activity than propolis extract. Propolis and caffeic acid inhibited NO production in macrophages, at concentrations without cytotoxicity. Furthermore, both propolis and caffeic acid suppressed LPS-induced signaling pathways, namely p38 MAPK, JNK1/2 and NF-NB. ERK1/2 was not affected by propolis extract and caffeic acid. In addition, propolis and caffeic acid did not induce hepatotoxicity at concentrations with strong anti-inflammatory potential. Conclusions:Propolis exerted an antioxidant and anti-inflammatory action and caffeic acid may be involved in its inhibitory effects on NO production and intracellular signaling cascades, suggesting its use as a natural source of safe anti-inflammatory drugs.

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Section: Introductionmentioning

confidence: 99%

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Búfalo

Ferreira

Costa

et al. 2013

Journal of Ethnopharmacology

152

102

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“…The liquid propolis was administered orally for 14 days before infected with Staphylococcus aureus non protein A. Infection with S. aures was conducted on the 15th day by injecting the bacterial inoculum dose of 1x10 9 cfu intraperitoneally. On the 15th day, all rats in each group were sacrificed to be taken peritoneal macrophages and blood, further tests phagocytic activity, NO production and antibodies.…”

Section: Animalsmentioning

confidence: 99%

“…[2,3,4,5,6]. This product has attracted the interest of researchers in recent decades because of the properties of biological and pharmacological, among others such as antimicrobial [7,8,9], antitumor [10], immunomodulator [11], anti-inflammatory [12], an antioxidant [13].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Studies of the Immunomodulator Effect of the Propolis Trigona spp. Extract in a Mouse Model

Kalsum¹

2017

IOSR JAVS

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Propolis helps boost the immune system naturally because propolis is rich in bioactive compounds and can help increase the production and activity of immune cells. This product has attracted the interest of researchers in recent decades because of the properties of biological and pharmacological, among others such as antimicrobial, antitumor, anti-inflammatory, antioxidant and immunomodulatory. One of bee species that potentially produce propolis in Indonesia is a local bee species Trigona spp. This study aimed to to determine the immunomodulatory effects of ethanol extract of propolis on the Sprague Dawley rats were infected by Staphylococcus aureus. Identification of the phytochemical compounds was undertaken to determine the bioactive compounds contained in propolis liquid. The effects of propolis were analyzed using the macrophage activity as determined by the phagocytic activity and by the production of nitric oxide (NO) in Sprague Dawley rat peritoneal macrophages and the production of antibodies. The results showed that the liquid propolis produces (a) an increase in the phagocytic index, (b) a significant increase in NO production, and (c)

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“…In recent studies, reports showed a wide range of biological and pharmacological properties of propolis including antibacterial (Kim & Chung, 2011;SiLvA et al, 2012), antiviral (BuFALo et al, 2009), antifungal (OtA et al, 2001, antioxidant (DA SiLvA FrozzA et al, 2013), and anti-inflammatory (SiLvA et al, 2012) characteristics.…”

mentioning

confidence: 99%

Chemical composition and antimicrobial activity of propolis collected from some localities of Western Algeria

Benhanifia¹,

Shimomura²,

Tsuchiya³

et al. 2014

Acta Alimentaria

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The chemical analysis and antibacterial activity of propolis collected from some parts of Western Algeria were investigated. The ethanolic extracts of propolis (EEP) were evaluated for further investigation. The major constituents in EEP were identified by high-performance liquid chromatography (HPLC) analysis. All EEP samples were active against Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus), but no activity was found against Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli). The mean diameters of growth inhibition of the EEP ranged between 8.05 and 21.4 mm. The propolis extract obtained from Sidi bel Abbés (SFS-SBA) was more active than other samples as well as showed unique HPLC profile. These results support the idea that propolis can be a promising natural food preservative in food industry and alternative candidate for management of bacterial infections caused by drug-resistant microorganisms.

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Anti-poliovirus activity ofBaccharis dracunculifoliaand propolis by cell viability determination and real-time PCR

Cited by 95 publications

References 21 publications

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Propolis and its constituent caffeic acid suppress LPS-stimulated pro-inflammatory response by blocking NF-κB and MAPK activation in macrophages

Preliminary Studies of the Immunomodulator Effect of the Propolis Trigona spp. Extract in a Mouse Model

Chemical composition and antimicrobial activity of propolis collected from some localities of Western Algeria

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