Anti-photoaging effects of flexible nanoliposomes encapsulated Moringa oleifera Lam. isothiocyanate in UVB-induced cell damage in HaCaT cells

Abstract

Skin photoaging is premature skin aging damage that occurs after repeated exposure to ultraviolet (UV) radiation. Although isothiocyanates extracted from the moringa tree ( Moringa oleifera Lam. ) (MITC) exhibit excellent effects against skin photoaging, its application is restricted because of its characteristics, such as extremely low water solubility, bioavailability, and easy degradation. Currently, flexible nanoliposomes have gained increasing interest as a biocompatible polymer for applications such as transdermal drug delivery. We prepare amphiphilic hyaluronic acid (HA) conjugated with ceramide (CE) to modify nanoliposomes for MITC (HACE/MITC NPs) delivery. The HACE/MITC nanoparticles (NPs) are prepared and characterized for entrapment efficiency, particle size, polydispersity index, zeta potential, in vitro release, in vivo skin permeation, and in vitro protective effect of photoaging. The zeta potential of MITC NPs and HACE/MITC NPs is −24.46 mV and −24.93 mV, respectively. After modification of HACE, the entrapment efficient of MITC liposome increased from 62.54% to 70.67%, and the particle size decreased from 266.1 nm to 192.8 nm. In vivo skin permeation, permeated drug increased from 49.42 to 71.40%. Moreover, the results showed that the entrapment of MITC in nanoliposomes improves its stability, efficacy, and skin permeation. Further, HACE/MITC NPs are favorable for uptake by HaCaT cells without requiring changes in cell morphology, which significantly improves the activities of antioxidant enzymes, scavenges UVB-induced reactive oxygen species, protects skin from damage, and reduces MMP-1, MMP-3, and MMP-9 expression caused by radiation-induced photoaging. Our results strongly suggest that flexible nanoliposomes successfully improved the cell membrane permeation of MITC, and that anti-photoaging and HACE/MITC NPs can potentially be used as candidates for photoaging therapy.

A Comprehensive Review of Moringa oleifera Bioactive Compounds—Cytotoxicity Evaluation and Their Encapsulation

“…By using this method, unstable liquid compounds can be converted into free-flowing stable powder. Core bioactive components have been encapsulated using a variety of techniques, including cross-linking gelation, freeze-drying, microemulsion, spray drying, and different nanoformulations (nanoliposomes) ( Figure 3 ) [ 72 , 73 , 74 ].…”

Moringa oleiferaLam.: a comprehensive review on active components, health benefits and application

“…13–15 Furthermore, the isothiocyanates contained in M. oleifera seeds also play a crucial role in anti-inflammatory, antioxidant, antibacterial and anticancer. 16 And the essential oil has coagulant effect that can accelerate wound healing. 17…”

“…3 Polyphenols in M. oleifera. [1] Cinnamic acid, [2] p-coumaric acid, [3] caffeic acid, [4] ferulic acid, [5] isoferic acid, [6] sinapic acid, [7] vanillic acid, [8] syringic acid, [9] gallic acid, [10] protocatechuic acid, [11] gentiolic acid, [12] chlorogenic acid, [13] niazirin, [14] salvianolic acid A, [15] salvianolic acid B, [16] quinic acid, [17] ellagic acid, [18] procyanidin, [19] diseases 11 and anti-epilepsy, 9 affect reproductive diseases 12 and other activities.…”

“…[13][14][15] Furthermore, the isothiocyanates contained in M. oleifera seeds also play a crucial role in anti-inammatory, antioxidant, antibacterial and anticancer. 16 And the essential oil has coagulant effect that can accelerate wound healing. 17 While previous research on M. oleifera mainly focused on the leaves and seeds, there has been limited discourse on its stems.…”

Nano–Liposomes Double Loaded with Curcumin and Tetrandrine: Preparation, Characterization, Hepatotoxicity and Anti–Tumor Effects

“…Studies have shown that, compared with inorganic delivery carriers, liposomes are more compatible and softer, suggesting that they are easier to be modified for drug delivery systems [ 13 ]. Thus, there are gradually more studies focusing on the modification of liposome structure to improve the feasibility of drug loading and the stability of the preparation [ 20 ].…”