Anti‐oxidation and immune responses in mice upon exposure to manganese superoxide dismutase expressed in silkworm larvae, <i>Bombyx mori</i> L

Yue, Wan‐Fu; Li, Guangli; Liu, Jianmei; Sun, Jianzhong; Sun, Hongxiang; Li, Xinghua; Wu, Xiyong; Miao, Yun‐gen

doi:10.1016/j.cellbi.2007.03.007

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…After centrifugation at 2,500 × g for 30 min at 4°C, the supernatant was collected for the measurement of antioxidant enzymes activities. The analysis of level of reactive oxygen species (ROS), activity of glutathione peroxidase (GPx), activity of glutathione reductase (GR), and activity of catalase (CAT) was carried out according to the methods previously reported [32–35]. The activity of superoxide dismutase (SOD) was determined by commercially available kit (Cayman, Ann Arbor, MI, USA).…”

Section: Methodsmentioning

confidence: 99%

Antihypertriglyceridemia and Anti-Inflammatory Activities ofMonascus-Fermented Dioscorea in Streptozotocin-Induced Diabetic Rats

Shi

Liao

Pan

2011

Experimental Diabetes Research

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The rice fermented by Monascus, called red mold rice (RMR), and has a long tradition in East Asia as a dietary staple. Monascus-fermented dioscorea called red mold dioscorea (RMD) contains various metabolites to perform the ability of reducing oxidative stress and anti-inflammatory response. We used Wistar rats and induced diabetes by injecting streptozotocin (STZ, 65 mg/kg i.p.). RMD was administered daily starting six weeks after disease onset. Throughout the experimental period, significantly (P < .05) lowered plasma glucose, triglyceride, cholesterol, free fatty acid and low density lipoprotein levels were observed in the RMD-treated groups. The RMD-treated diabetic rats showed higher activities of glutathione disulfide reductase, glutathione reductase, catalase and superoxide dismutase (P < .05) in the pancreas compared with the diabetic control rats. RMD also inhibited diabetes-induced elevation in the levels of interleukin (IL)-1β, IL-6, interferon-γ and tumor necrosis factor-α. Pancreatic β-cells damaged by STZ in the RMD supplemented groups were ameliorated. The results of this study clearly demonstrated that RMD possesses several treatment-oriented properties, including the control of hyperglycemia, antioxidant effects, pancreatic β-cell protection and anti-inflammatory effects. Considering these observations, it appears that RMD may be a useful supplement to delay the development of diabetes and its complications.

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Section: Methodsmentioning

confidence: 99%

Antihypertriglyceridemia and Anti-Inflammatory Activities ofMonascus-Fermented Dioscorea in Streptozotocin-Induced Diabetic Rats

Shi

Liao

Pan

2011

Experimental Diabetes Research

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“…We have also investigated the effect of silkworm powder containing SOD on the antioxidation and the immune system of the mouse, focusing on hemolysin response, hemagglutination against SRBC, PFC assay and the dose-dependent increase of phagocytic activity. All treated mice showed significant enhancement of immunity (Yue et al, 2007(Yue et al, , 2008.…”

Section: Discussionmentioning

confidence: 97%

Demonstration of protein absorption in the intestinal epithelium of fish and mice by laser scanning confocal microscopy

Yue

Zhou

Malik

et al. 2010

Biological Chemistry

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Selective permeability for small proteins and oligopeptides occurs in the intestinal epithelium of many animal species and humans. Whole proteins are sometimes endocytosed and undergo partial hydrolysis in intestinal epithelial cells with the probable release of essential oligopeptides into the bloodstream. Increased permeability to certain proteins can cause asthma and other metabolic disorders. Permeable proteins have also been successfully used to deliver vaccines or drugs via oral consumption. Protein absorption has been inferred in many cases and demonstrated in some cases by histochemical, tracer, and analytical techniques. However, the nature and importance of protein absorption remains largely unknown. Here, we demonstrate the movement of two lumenal proteins (GFP: 26 kDa and OFP: 23 kDa) across the intestinal epithelium of fish and mice using laser scanning confocal microscopy. The results provide evidence that small proteins can be taken up intact by intestinal epithelial cells, even though large proteins are digested to single amino acids or protein fragments before they are absorbed. Our results suggest that it is possible to orally administer some small proteinous medicines for therapeutic purposes.

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Effects of silkworm larvae powder containing manganese superoxide dismutase on immune activity of mice

Yue

Liu

et al. 2007

Mol Biol Rep

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To study the function of silkworm larvae powder containing superoxide dismutase and potential practical development, we investigated the safety assessment and effects on immune activity of mice such as the growth of immunity-related organs, delayed-type hypersensitivity (DTH) and charcoal particle clearance ability. The mean body weights in treated mice were significantly heavier than that of control, meanwhile, the ratio of splenocytes/body weight and the thoracic gland/body weight in treated mice was significantly enhanced after 30 days treated with silkworm larvae powder containing manganese superoxide dismutase. The treated mice resulted in a profound activation of the DTH and charcoal particle clearance, and indicated the treated mice have stronger phagocytic activity to exogenous materials. Our data also indicated the feeding treatment was safe with 360 folds of recommended human dosage in acute toxic test. In long-term test, there were no effects of silkworm larvae powder containing SOD on treated mice's growth and inside organs as long as 90 days. Further the electronic microscope investigation showed the intestine, liver, splenocyte and stomach in mice were no obvious changes both in organs and sub-organs such as nucleus, endoplasmic reticulum, mitochondrion, Golgi and peroxisomes after treated for as long as 90 days.

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Anti‐oxidation and immune responses in mice upon exposure to manganese superoxide dismutase expressed in silkworm larvae, Bombyx mori L

Cited by 8 publications

References 17 publications

Antihypertriglyceridemia and Anti-Inflammatory Activities ofMonascus-Fermented Dioscorea in Streptozotocin-Induced Diabetic Rats

Antihypertriglyceridemia and Anti-Inflammatory Activities ofMonascus-Fermented Dioscorea in Streptozotocin-Induced Diabetic Rats

Demonstration of protein absorption in the intestinal epithelium of fish and mice by laser scanning confocal microscopy

Effects of silkworm larvae powder containing manganese superoxide dismutase on immune activity of mice

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