Anti-osteoporotic activity of aqueous-methanol extract of Berberis aristata in ovariectomized rats

Yogesh, H S; Chandrashekhar, V. M.; Katti, H.R.; Ganapaty, S; Raghavendra, HL; Gowda, Girish; Goplakhrishna, B.

doi:10.1016/j.jep.2010.12.013

Cited by 51 publications

(26 citation statements)

References 16 publications

(21 reference statements)

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“…The number of positively stained osteoclasts in the sections of the median portion of the whole femora may be recorded [15]. The femur section was examined for any histological changes like architecture, bone compactness, fragility with disruptive, lytic changes, thinning of the trabeculae resulting in intertrabecular spaces widening, increased ossification, mineralization and increased osteoclastic activity and reduced bone resorption, which indicates the recovery with essential features of normal bone [67].…”

Section: Histopathologymentioning

confidence: 99%

“…In addition, increase in the concentration of calcium, phosphorous and creatinine in urine is a supporting factor for bone loss in the OVX rats [72]. Hence, urine samples are collected in metabolic cages without providing food 1 day before euthanizing the animals and acidified with 2 mL 1 mol/L HCl, then the level of urine calcium, phosphorus and creatinine is estimated using automatic or semiautomatic analyzer and using commercially available test kits [67]. Urinary excretion of Ca, P and DPD is also expressed as the ratio to Cr concentration (Ca/Cr; P/Cr; DPD/Cr) [59].…”

Section: Urine Analysesmentioning

confidence: 99%

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Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Satpathy

Patra

Ahirwar

2015

Journal of Complementary and Integrative Medicine

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Postmenopausal osteoporosis, a silent epidemic, has become a major health hazard, afflicting about 50% of postmenopausal women worldwide and is thought to be a disease with one of the highest incidences in senile people. It is a chronic, progressive condition associated with micro-architectural deterioration of bone tissue that results in low bone mass, decreased bone strength that predisposes to an increased risk of fracture. Women are more likely to develop osteoporosis than men due to reduction in estrogen during menopause which leads to decline in bone formation and increase in bone resorption activity. Estrogen is able to suppress the production of proinflammatory cytokines like interleukin (IL)-1, IL-6, IL-7 and tumor necrosis factor (TNF-α). This is why these cytokines are elevated in postmenopausal women. In this review article we have made an attempt to collate the various methods and parameters most frequently used for screening of antiosteoporotic activity in postmenopausal osteoporosis. Pertaining to ovariectomized animal model, this is the most appropriate model for studying the efficacy of different drugs to prevent bone loss in postmenopausal osteoporosis.

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Section: Histopathologymentioning

confidence: 99%

Section: Urine Analysesmentioning

confidence: 99%

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Satpathy

Patra

Ahirwar

2015

Journal of Complementary and Integrative Medicine

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“…Ovariectomy results in a drastic decrease in uterine weight and vagina thickness at the beginning of the treatment (18,32), and these changes worsen during the period of subchronical treatment allowing dyslipidemia and decrease in bone mineral density and biomechanical strength as seen in postmenopausal women that we named the postmenopausal-like model. The model has been used previously by different laboratories to investigate the effects of natural and synthetic products on menopausal symptoms (15,33,34). These changes are mostly due to estrogen deficiency.…”

Section: Fig 4 Effects Of 3-day Treatment Withmentioning

confidence: 99%

Effects of Millettia macrophylla (Fabaceae) Extracts on Estrogen Target Organs of Female Wistar Rat

et al. 2013

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Abstract. The present study aims to determine the estrogenicity of Millettia macrophylla, a Cameroonian medicinal plant, in ovariectomized rats and to investigate the underlying mechanisms, in order to justify scientifically its traditional use. To accomplish this objective, we used dichloromethane (DCM) and methanol (MeOH) extracts of the stem bark of M. macrophylla. In the cell culture based assay, the MeOH extract significantly transactivated estrogen receptor a (ERa) and estrogen receptor b (ERb); in addition, the estrogen-like effects of both, DCM and MeOH extracts, could be inhibited in vitro by the pure ER antagonist ICI 182,780, indicating that these effects were primarily mediated through ERs. In animal experiments, both DCM and MeOH extracts significantly increased the uterine and vaginal epithelial heights in the 3-day treatment assay, while only the MeOH extract exhibited such effects in the sub-chronic treatment regimen. Furthermore, the MeOH extract significantly decreased fasting serum triglycerides, total cholesterol levels and artherogenic risk in the sub-chronic treatment. These results indicate that M. macrophylla extracts have estrogen-like effects supporting their traditional use in

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“…8 Hormone replacement therapy (HRT) is a treatment for the prevention of bone loss and for restoring the rate of bone resorption and formation. 9 However, long-term HRT may increase a patient's risk of developing breast cancer, endometrial cancer, thromboembolic events, or vaginal bleeding. 10 Therefore, because of women's reluctance to comply with HRT, safer ingredients originating from foods or beverages such as tea are needed for the treatment or prevention of menopausal symptoms, such as osteoporosis.…”

Section: ■ Introductionmentioning

confidence: 99%

Inhibitory Effects of the Leaves of Loquat (Eriobotrya japonica) on Bone Mineral Density Loss in Ovariectomized Mice and Osteoclast Differentiation

Tao

Furuta

Nagata

et al. 2014

J. Agric. Food Chem.

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The loquat, Eriobotrya japonica Lindl. (Rosaceae), is a small tree native to Japan and China that is widely cultivated for its succulent fruit. Its leaves are used as an ingredient of a tasty tea called "Biwa cha" in Japanese. The anti-osteoporosis effects of the leaves of loquat in vitro and in vivo have been investigated. After 15 days of feeding normal diet or diet supplemented with 5% loquat leaves, the body weight, viscera weights, and bone mineral density (BMD) of both groups of eight ovariectomized (OVX) mice were compared. The result showed that the loss of BMD in loquat-fed mice was significantly prevented in three parts of the body, especially in the trabecular bone of the head (P < 0.05), abdomen (P < 0.01), and lumbar (P < 0.05) compared to the control group. No hypertrophy in the uterus by the loquat leaves diet was observed. The effect of the extract (447.25 g) prepared from the dried leaves of loquat (2.36 kg) was further studied on RANKL-induced osteoclast differentiation and cell viability. The extract suppressed the differentiation of osteoclasts under 50, 125, 250, and 500 μg/mL. Through bioactivity-guided fractionation, ursolic acid (1) was isolated and inhibited osteoclast differentiation under 4 and 10 μg/mL. It was concluded that loquat leaves possess the potential to suppress ovariectomy-induced bone mineral density deterioration.

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Anti-osteoporotic activity of aqueous-methanol extract of Berberis aristata in ovariectomized rats

Cited by 51 publications

References 16 publications

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Effects of Millettia macrophylla (Fabaceae) Extracts on Estrogen Target Organs of Female Wistar Rat

Inhibitory Effects of the Leaves of Loquat (Eriobotrya japonica) on Bone Mineral Density Loss in Ovariectomized Mice and Osteoclast Differentiation

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