Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte

Kim, Soonhee; Lee, Myoung-Su; Kim, Sung Hee; Yang, Hye‐Jeong; Kim, Minjung; Kim, Hak‐Soo; Ha, Joohun; Kim, Myung Sunny; Kwon, Dae Young

doi:10.1016/j.bbrc.2007.10.125

Cited by 163 publications

(139 citation statements)

References 24 publications

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…Some studies have reported that ginseng increases the PPARc mRNA expression in adipose tissue (Park et al 2005;Mollah et al 2009) and in some studies, it decreases. For instance, ginsenoside Rb1, Rg1, Rg3 and Rh2 decreased the lipid accumulation and PPARc mRNA expression levels in 3T3-L1 adipocytes (Hwang et al 2007;Park et al 2008;Hwang et al 2009), and ginsenoside Rh2 and Rg3 have been reported as an antagonist for PPARc (Hwang et al 2007;Hwang et al 2009). In our study, expression levels of mRNA and protein expression both of PPARc2 and SREBP-1 levels were decreased in WAT of KGE fed mice.…”

Section: Discussionmentioning

confidence: 99%

Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese mice

et al. 2010

View full text Add to dashboard Cite

The present study examined the antiobesity effect and mechanism of action of Korean white ginseng extracts (KGE) using high-fat diet (HFD)-induced obese mice. Mice were fed a low-fat diet (LFD), HFD or HFD containing 0.8 and 1.6% (w/ w) KGE diet (HFD ? 0.8KGE and HFD ? 1.6KGE) for 8 weeks. We also examined the effects of KGE on plasma triglyceride (TG) elevation in mice administrated with oral lipid emulsion. Body weight gain and white adipose tissue (WAT) weight were significantly decreased in the HFD ? 1.6KGE group, compared with the HFD group. The plasma TG levels were also significantly reduced in both HFD ? 0.8KGE and HFD ? 1.6KGE groups, while leptin levels were significantly decreased in only the HFD ? 1.6KGE group, compared with the HFD group. The HFD ? 1.6KGE group showed significantly lower mRNA levels of lipogenesis-related genes, including peroxisome proliferator-activated receptorc2 (PPARc2), sterol regulatory element binding protein-1c (SREBP-1c), lipoprotein lipase (LPL), fatty acid synthase (FAS) and diacylglycerol acyltransferase 1 (DGAT1), compared with the HFD group. In addition, a dose of 1000 mg/kg KGE inhibited the elevation of plasma TG levels compared with mice given the lipid emulsion alone. These results suggest that the anti-obesity effects of KGE may be elicited by regulating expression of lipogenesis-related genes in WAT and by delaying intestinal fat absorption.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese mice

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In this study, the HFD-FMIX group showed reduced plasma insulin levels compared to the HFD group. It is still unclear whether supplementation with the fermented mixture had a direct effect in decreasing FSM: 10% skim milk media fermented by L. acidophilus 3) FMIX: 10% skim milk media with 1% Bokbunja and 1% red ginseng extract fermented by L. acidophilus 3) Data are presented as a mean ± standard deviation. Means with the same alphabet in each column are not significantly different at p < 0.05 using one-way ANOVA 4) ND = Not detected.…”

Section: Discussionmentioning

confidence: 99%

“…However, multiple studies reported that treatment with red ginseng extract or its functional constituents, saponins, led to a reduction in the mRNA levels of angiogenic factors (e.g., VEGF-A and FGF-2) and matrix metalloproteinases (MMPs) (e.g., MMP-2 and MMP-9), and the expression of hypothalamic neuropeptide Y (NPY) in vivo [2][3][4]25]. In addition, immature black raspberry contains large amounts of ellagic acid, which can regulate the protein levels of Nrf2, NF-κB and CPT1 in an HFD-induced rat model of metabolic syndrome [26], and has anti-obesity effects [27].…”

Section: Discussionmentioning

confidence: 99%

“…Several groups reported that the anti-obesity effect is attributed to ginseng saponins [2][3][4], which are classified into protopanaxadiol group (ginsenosides: Ra, Rb, Rc, Rd, Rg3, Rh2, and Rs) and protophanxatriol group (ginsenosides: Re, Rf, Rg1, Rg2, and Rh1) depending on their chemical structures. Red ginseng is known to contain large amounts of Rg2, Rg3, Rh1, and Rh2 ginsenosides that are effectively produced during the heating process.…”

Section: Introductionmentioning

confidence: 99%

“…Red ginseng is known to contain large amounts of Rg2, Rg3, Rh1, and Rh2 ginsenosides that are effectively produced during the heating process. Rg3 and Rh2 ginsenosides have anti-obesity effects in vitro [3,4].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Obesity Effect of a Mixture of Skim Milk, Red Ginseng Extract, and Black Raspberry Extract Fermented with Lactobacillus acidophilus on High-Fat Diet-Fed Obese Mice

Yum¹,

Lee²,

Oh³

et al. 2016

J Food Chem Nanotechnol

View full text Add to dashboard Cite

Enzymatic transformation of ginseng leaf saponin by recombinant β‐glucosidase (bgp1) and its efficacy in an adipocyte cell line

Huq

Siraj

Kim

et al. 2015

Biotech and App Biochem

View full text Add to dashboard Cite

The major ginseng leaf saponins are transformed into the more pharmacologically active minor ginsenosides by recombinant β-glucosidase enzyme bgp1. Ginseng leaves contain six major ginsenosides: Rg1, Re, Rb1, Rb2, Rc, and Rd. Among these Rg1, Re and Rd are the most abundant. Within 3 H of incubation, all dominant major ginsenosides found in ginseng leaf had decomposed and been converted into the more active minor ginsenosides (i.e., 100% of Rg1, Re, and Rd were decomposed and converted into Rh1, Rg2, and Rg3, respectively). The recombinant β-glucosidase enzyme (bgp1) hydrolyzed all glucose moieties attached to the C-20 position of the ginsenosides Rg1, Re, Rb1, Rd, and F1. The transformed product contains pharmacologically active minor ginsenosides Rh1, Rg2, Rg3, F1, and protopanaxatriol. This transformed product was used to investigate the effects on the 3T3-L1 adipocyte cell line. The cytotoxicity assay did not show any toxicity, even when used at a concentration of 100 μg/mL. Adipogenesis was shown to decrease in response to bioconverted leaf saponin in a dose-dependent manner.

show abstract

Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte

Cited by 163 publications

References 24 publications

Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese mice

Effects of Korean white ginseng extracts on obesity in high-fat diet-induced obese mice

Anti-Obesity Effect of a Mixture of Skim Milk, Red Ginseng Extract, and Black Raspberry Extract Fermented with Lactobacillus acidophilus on High-Fat Diet-Fed Obese Mice

Enzymatic transformation of ginseng leaf saponin by recombinant β‐glucosidase (bgp1) and its efficacy in an adipocyte cell line

Contact Info

Product

Resources

About