Anti-obesity effect of Polygala tenuifolia

Abstract

This study was performed in order to investigate the anti-obesity effect of Polygala tenuifolia on lipid mechanism in 3T3-L1 adipocytes. The chemical composition of the P. tenuifolia was analyzed in order to assess its nutritional value. Total dietary fiber was the highest among the proximate component of the P. tenuifolia. These results showed that the P. tenuifolia may be used as a potential functional ingredient for anti-obesity effect. Intracellular lipid droplets in the adipocyte were stained with oil-red O dye and quantified. In comparison to the control, lipid accumulation was significantly decreased by 40.1% and 22.4% when treated with the water extract and 70% EtOH extract of the P. tenuifolia at the concentration of 10 μg/mL, respectively. The anti-adipogenic effect of the water extract was stronger than that of the 70% EtOH extract. The gene expression levels were measured via Western blot and real-time PCR. As a result, the water extract was found to have decrease the gene expression of SREBP-1c, PPAR, C/EBP α, FAS, ACC in a dose-dependent manner. These indicate that the water extract inhibits pre-adipocyte differentiation and adipogenesis by blocking the SREBP-1c gene expression in 3T3-L1 cells. Therefore, P. tenuifolia can be used as an effective anti-obesity agent. Key words：Polygala

Effect of Centella asiatica Extract on Anti-obesity Suppression via Inhibition of Adipogenesis-related Gene Expression in Preadipocyte

“…As shown in Figure 3, these were inhibited during the 1.0 mg/mL treatment by 1.52, 1.81, 1.13, and 1.18 times, respectively, compared to the control group. The inhibition of CEBP-α expression in preadipocytes via CHE treatment was 2.2 times higher than that reported in Lee et al, where it was demonstrated that the inhibition of preadipocyte differentiation produced anti-obesity effects (Hwang et al, 2014). Additionally, Wang et al (1995) confirmed that lipid accumulation did not occur when CEBP-α was eliminated from the in vivo model, further demonstrating that the inhibition of this gene is essential to obtain the antiobesity effect as it upregulates preadipocyte differentiation (Wang et al, 1995).…”

Anti-Obesity Effect of Polygalin C Isolated from Polygala japonica Houtt. via Suppression of the Adipogenic and Lipogenic Factors in 3T3-L1 Adipocytes

“…Recently, bioactive compounds have been extensively studied, and provide not only essential roles as nutrients but also biochemical and pharmacological functions beneficial to human health [ 43 ]. In previous studies, Polygala genus plants have been reported to possess anti-obesity effects [ 29 , 44 ]. However, there are no studies on the anti-obesity activities and mechanisms of PC isolated from P. japonica Houtt.…”

A 4-week Repeated dose Oral Toxicity Study of Mecasin in Sprague-Dawley Rats to Determine the Appropriate Doses for a 13-week, Repeated Toxicity Test

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