Anti-nociceptive properties in rodents and the possibility of using polyphenol-rich fractions from sida urens L. (Malvaceae) against of dental caries bacteria

Abstract

BackgroundSida urens L. (Malvaceae) is in flora of Asian medicinal herbs and used traditionally in West of Burkina Faso for the treatment of infectious diseases and particularly used against, dental caries bacteria, fever, pain and possesses analgesic properties. This study was conducted to reveal the antibacterial effect against dental caries bacteria on the one hand, and evaluate their analgesic capacity in experimental model with Swiss mice and on the other hand, with an aim to provide a scientific basis for the traditional use of this plant for the management of dental caries bacteria.MethodThe antibacterial assays in this study were performed by using inhibition zone diameters, MIC (Minimum inhibitory concentration) and MBC (Minimal bactericidal concentration) methods. On the whole the dental caries bacteria (Gram-positive and Gram-negative bacterial strains) were used. Negative control was prepared using discs impregnated with 10% DMSO in water and commercially available Gentamicin from Alkom Laboratories LTD was used as positive reference standards for all bacterial strains. In acute toxicity test, mice received doses of extract (acetone/water extract) from Sida urens L. by intraperitoneal route and LD50 was determined in Swiss mice. As for analgesic effects, acetic acid writhing method was used in mice. The acetic acid-induced writhing method was used in mice with aim to study analgesic effects.ResultsThe results showed that the highest antibacterial activities were founded with the polyphenol-rich fractions against all bacterial strains compared to the standard antibiotic. About preliminary study in acute toxicity test, LD50 value obtained was more than 5000 mg/kg b.w. Polyphenol-rich fractions produced significant analgesic effects in acetic acid-induced writhing method and in a dose-dependent inhibition was observed.ConclusionThese results validate the ethno-botanical use of Sida urens L. (Malvaceae) and demonstrate the potential of this herbaceous as a potential antibacterial agent of dental caries that could be effectively used for future health care purposes.

Synthesis and antimicrobial evaluation of promising 7-arylamino-5,8-dioxo-5,8-dihydroisoquinoline-4-carboxylates and their halogenated amino compounds for treating Gram-negative bacterial infections

“…50 The ratio of MBC/MIC was used to determine whether the mechanism of action of the derivatives was bactericidal (MBC/MIC ¼ 1 or 2) or bacteriostatic (MBC/MIC ¼ 4 or 16). 51 Hemocompatibilityhemolysis assay. The experiment was conducted according to Sathler and collaborators (2014).…”

Comparative Study of the Antinociceptive Action of Amitriptyline With Fluoxetine and Evaluation of Their Probable Mechanism of This Action in Albino Mice

“…Pain sensation in acetic acid induced writhing method is due to prostaglandin biosynthesis (Via cyclooxygenase pathway) triggered by localized inflammatory reaction [39,40]. So, agents which reduce the number of writhing will render their effect preferably by inhibition of prostaglandin biosynthesis [39,40]. In our study, both the antidepressants were effective in preventing acetic acid-induced writhing movements in mice.…”

“…But the exact mechanism of how peripheral 5-HT3 modulation is involved in the peripheral antinociceptive action of both the drugs is still not clear. Pain sensation in acetic acid induced writhing method is due to prostaglandin biosynthesis (Via cyclooxygenase pathway) triggered by localized inflammatory reaction [39,40]. So, agents which reduce the number of writhing will render their effect preferably by inhibition of prostaglandin biosynthesis [39,40].…”

Sida tuberculata extract reduces the nociceptive response by chemical noxious stimuli in mice: Implications for mechanism of action, relation to chemical composition and molecular docking

“…In a recent work about antinociceptive properties, Bonjardim et al () demonstrate that Sida cordifolia has a pronounced antinociceptive activity on orofacial nociception in mice. In another study, the polyphenol‐rich fractions obtained from Sterculia urens produced significant analgesic effects in acetic acid‐induced writhing method (Konaté et al, ). S. cordifolia also reduced the prostanoid release in macrophage cell model, being verified antinociceptive and anti‐inflammatory activity in models induced by acetic acid and carrageenan (Martins et al, ; Sutradhar, Rahman, Ahmad, & Bachar, ).…”