Sida tuberculata extract reduces the nociceptive response by chemical noxious stimuli in mice: Implications for mechanism of action, relation to chemical composition and molecular docking

Abstract

Sida tuberculata R.E.Fr. (Malvaceae) is a medicinal plant widely found in Southern Brazil, and popularly used for inflammatory disorders and to pain relief. A phytochemical analysis followed by an investigation about antinociceptive potential and mechanism of action were performed with leaves and roots extracts. Methanolic extracts, designated as S. tuberculata leaves extract (STLE) and S. tuberculata roots extract, were analyzed both by UHPLC-MS. The in vivo antinociceptive potential of STLE (10-300 mg kg −1 ) was assessed in mice subjected to the acetic acid-induced abdominal writhes and formalin model. Agonist/antagonist tests and computational docking suggest the involvement of opioid and adenosinergic systems. The main chemical class detected on extracts was the ecdysteroids, and 20-hydoxyecdysone (20HE) was confirmed as the major phytoconstituent. The pretreatment with STLE (100 mg kg −1 ) reduced more than 70% abdominal contortions induced by acetic acid model and produced significant inhibition on formalin-induced licking response. The mechanism of action study revealed STLE might act through opioid and adenosine systems. Molecular docking suggested kaempferol derivative and 20HE might interacting with μ-opioid receptor. Thus, the results suggest the existence of antinociceptive potential from S. tuberculata extracts being in accordance to the traditional use.

Flavonoid‐Rich Fraction from Croton blanchetianus (Euphorbiaceae) Leaves Exerts Peripheral and Central Analgesic Effects by Acting via the Opioid and Cholinergic Systems

“…[3][4][5] Efforts directed at discovering new and safer therapies have revealed the potential of several medicinal plants, which have been traditionally used as analgesics, as alternatives. [6] Some studies investigating the mechanisms of action underlying the analgesic effects of medicinal plants have reported involvement of the opioid and adenosinergic systems [7,8] and the inhibition of the synthesis of prostaglandins and nociceptive substances. [9,10] Flavonoids are secondary metabolites widely found in plants, with several biological effects, such as analgesic and anti-inflammatory effects.…”

Chemical Composition and In Vitro Antioxidant Activity of Sida rhombifolia L. Volatile Organic Compounds

“…Endemic and medicinal plants in this rich flora constitute a vital place. The genus Sida (Malvaceae) has about 200 species generally scattered in tropical and subtropical areas, whose pharmacological uses have been supported by several studies [ 4 , 5 , 6 ]. S. rhombifolia L., commonly known as teaweed and Queensland hemp, is an erect perennial shrub bearing yellowish flowers.…”