Anti-Nociceptive Effects of an Ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn in Mice: Involvement of Adenosinergic Mechanisms

Woode, Eric; Amoateng, Patrick; Ansah, Charles; Duwiejua, M.

doi:10.3923/jpt.2009.17.29

Cited by 20 publications

(24 citation statements)

References 34 publications

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“…[3] The whole plant extract have been reported to possess potent anti-inflammatory, [4] antibacterial and antioxidant [5] and antinociceptive. [6] Free radicals and associated lipid peroxidation are implicated in epilepsy and inflammation [7] and the role of antioxidants in the management of conditions caused by oxidative stress have been reported. [8][9][10][11] We have demonstrated the anticonvulsant effects of the hydro-ethanolic extract of the plant in our laboratory.…”

Section: Free Radical Scavenging and Anti-lipid Peroxidative Effects mentioning

confidence: 99%

Free Radical Scavenging and Anti-lipid Peroxidative Effects of a Hydro-ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn (Asteraceae)

Amoateng

Koffuor

Sarpong

et al. 2011

Free Radicals and Antioxidants

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Background: Synedrella nodiflora (L.) Gaertn. (Asteraceae), a native Ghanaian shrub, has been used for the treatment of epilepsy, hiccup and threatened abortion. The present study aimed at investigating the possible mechanisms of antioxidant effects of the hydro-ethanolic extract of the whole plant. Methods: Total phenolic content was determined using the FolinCiocalteau assay and the antioxidant capacity by the phosphomolybdenum method. The antioxidant activity was evaluated by the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays, reducing power assay and inhibition of linoleic acid peroxidation and lipid peroxidation in pentylenetetrazole (PTZ)-kindled rat brains. Results: The extract (0.1-3.0 mg ml -1 ) was found to contain phenolic compounds which could be responsible for the antioxidant properties since the coefficient of correlation between the Total Phenolic Content (TPC) and the Total Antioxidant Capacity (TAC) was high (r 2 = 0.9908). Both n-propyl gallate (0.001-0.03 mg ml -1 ), a reference antioxidant and the extract (0.1-3 mg ml -1 ) exhibited antioxidant properties by reducing Fe 3+ to Fe 2+ in the reducing power test, scavenged DPPH free radicals and effectively inhibited linoleic acid autoxidation and also inhibited lipid peroxidation in PTZ-kindled rat brains. Conclusions:These findings suggest that hydro-ethanolic extract of Synedrella nodiflora contains antioxidant principles which may contribute to its traditional use in epilepsy management.

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Section: Free Radical Scavenging and Anti-lipid Peroxidative Effects mentioning

confidence: 99%

Free Radical Scavenging and Anti-lipid Peroxidative Effects of a Hydro-ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn (Asteraceae)

Amoateng

Koffuor

Sarpong

et al. 2011

Free Radicals and Antioxidants

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“…This paw pressure test was conducted according to the method described by Woode et al 21 and Zakaria et al 22 The rats were placed on the Ugo Basile dynamic plantar aesthesiometer for 20 minutes prior to the experiment to enable them to adapt to their surroundings. Around 30 minutes after oral administration of the treatment drug, 50 μL of 1% carrageenan was injected into their right hind paws via intraplantar injection.…”

Section: Randall-sellito Paw Pressure Test (Mechanical Hyperalgesia)mentioning

confidence: 99%

Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice

Goh

Tang

Chiong

et al. 2014

IJN

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Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall–Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall–Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac.

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“…The highest therapeutic dose of the hydro-ethanolic extract demonstrating anticonvulsant and related neuropharmacological properties is lower (i.e. 1000 mg kg -1 ) (Woode et al, 2009, Amoateng et al, 2012, than the doses used in this study (which are 1600, 3200 and 6400 mg kg -1 ). Thus this present study only demonstrates the effects of acute administration of high doses of the extract beyond 1000 mg kg -1 .…”

Section: Parametermentioning

confidence: 52%

“…The hydro-ethanolic extract of the whole plant has been found to possess anticonvulsant (Amoateng et al, 2012), sedative (Woode et al, 2011), in vitro antioxidant and free radical scavenging properties and anti-nociceptive properties (Woode et al, 2009). To explore the plant for further pre-clinical anti-epileptic drug discovery and development, it is important to investigate the toxicity of the plant.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and haematological changes following an acute toxicity study of a hydro-ethanolic whole plant extract of <i>Synedrella nodiflora</i> (L) Gaertn in male Sprague-Dawley rats

Adjei¹,

Amoateng²,

Osei‐Safo³

et al. 2014

J. Med. Biomed. Sci.

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Synedrella nodiflora (L) Gaertn (family Asteraceae), a common weed in Ghana, is traditionally used for the management of epilepsy, hiccup and threatened abortion. To further promote the ethnopharmacological uses of the plant, an acute toxicity of a hydro-ethanolic whole plant extract was assessed in male Sprague-Dawley rats. The lethal dose (LD50) and effects of a single oral administration of the extract (1600, 3200 and 6400 mg kg -1 ) on haematological and serum biochemical parameters were measured. The extract produced no mortality in the rats treated during a 48-hour examination and after a subsequent 12-day assessment. Thus the LD50 was indicated as being greater than 6400 mg kg -1 . The extract also did not significantly affect any of the haematological and serum biochemical indices. This result suggests that acute oral administration of the hydroethanolic extract of Synedrella nodiflora is virtually non-toxic in male Sprague-Dawley rats under normal laboratory conditions.

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Anti-Nociceptive Effects of an Ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn in Mice: Involvement of Adenosinergic Mechanisms

Cited by 20 publications

References 34 publications

Free Radical Scavenging and Anti-lipid Peroxidative Effects of a Hydro-ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn (Asteraceae)

Free Radical Scavenging and Anti-lipid Peroxidative Effects of a Hydro-ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn (Asteraceae)

Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice

Biochemical and haematological changes following an acute toxicity study of a hydro-ethanolic whole plant extract of <i>Synedrella nodiflora</i> (L) Gaertn in male Sprague-Dawley rats

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