Free Radical Scavenging and Anti-lipid Peroxidative Effects of a Hydro-ethanolic Extract of the Whole Plant of Synedrella nodiflora (L.) Gaertn (Asteraceae)

Abstract

Background: Synedrella nodiflora (L.) Gaertn. (Asteraceae), a native Ghanaian shrub, has been used for the treatment of epilepsy, hiccup and threatened abortion. The present study aimed at investigating the possible mechanisms of antioxidant effects of the hydro-ethanolic extract of the whole plant. Methods: Total phenolic content was determined using the FolinCiocalteau assay and the antioxidant capacity by the phosphomolybdenum method. The antioxidant activity was evaluated by the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging assays, reducing power assay and inhibition of linoleic acid peroxidation and lipid peroxidation in pentylenetetrazole (PTZ)-kindled rat brains. Results: The extract (0.1-3.0 mg ml -1 ) was found to contain phenolic compounds which could be responsible for the antioxidant properties since the coefficient of correlation between the Total Phenolic Content (TPC) and the Total Antioxidant Capacity (TAC) was high (r 2 = 0.9908). Both n-propyl gallate (0.001-0.03 mg ml -1 ), a reference antioxidant and the extract (0.1-3 mg ml -1 ) exhibited antioxidant properties by reducing Fe 3+ to Fe 2+ in the reducing power test, scavenged DPPH free radicals and effectively inhibited linoleic acid autoxidation and also inhibited lipid peroxidation in PTZ-kindled rat brains. Conclusions:These findings suggest that hydro-ethanolic extract of Synedrella nodiflora contains antioxidant principles which may contribute to its traditional use in epilepsy management.

Effect of extraction solvents on the biomolecules and antioxidant properties of Scorzonera undulata (Asteraceae): Application of factorial design optimization phenolic extraction

“…AFS.2015.4.32 high phenolic compounds content showed by this extract. Amoateng et al (2011) reported that DPPH scavenging ability of Synedra nodifl ora (Asteraceae family) extracted in ethanol solvent (70%) is about 0.31 mg/ml of extract, suggesting that our results are more important. In the DPPH…”

Antioxidant and Anti-Proliferative Effects of an Ethyl Acetate Fraction of the Hydro-Ethanolic Extract of Synedrella Nodiflora (L) Gaertn

“…The total phenolic content of the crude extract (SNE) was assayed by the Folin Ciocalteu's method, as published by Amoateng et al, 2011 [5] with slight modifications. An aliquot (10 µl) of the extract (20 mg/ml) was placed into an Eppendorf tube, to which 0.79 ml of distilled water was added.…”

“…In Ghana, the whole plant is boiled, and the aqueous extract drunk for the treatment of neurological conditions such as epilepsy, whiles the leaves are used for threatened abortion, hiccup, laxative and as feed for livestock [1]. The crude hydro-ethanolic extract of this whole plant has been found to possess anticonvulsant [3], sedative [4], antioxidant [5], anti-nociceptive properties [6,7] and anti-proliferative effects on onion root and shoot apical meristem and wister rat bone marrow cells [8]. Hence, this study seeks to investigate further the antioxidant potential and anti-proliferative activities of various fractions of the hydro-ethanolic extract of this plant.…”

High pressure extraction increases the antioxidant potential and in vitro bio-accessibility of bioactive compounds from discarded blueberries

“…For example, after in vitro digestion, the polyphenols could have been degraded by the alkaline pH and subsequent decrease in the TPC, TFC and the antioxidant capacity (Bobrich et al, 2014). The degradation of bioactive compounds may also come from enzymatic reactions in the sample, resulting in the degradation of bioactive compounds (Amoateng, Assumeng-Koffuor, Sarpong, & Oteng-Agyapong, 2011). Likewise, the processing effect on potential bio-accessibility of antioxidant capacity and bioactive compounds can exert a positive or negative effect, depending on the food matrix in the processing conditions (Lafarga, Villaró, Bobo, Simó, & Aguiló-Aguayo, 2019).…”