Anti-Nociceptive and Anti-Inflammatory Activities of Extracts from the Stem Bark of Croton macrostachyus (Euphorbiaceae) in Mice and Rats

Kim, Albert; Mbiantcha, Marius; Nguelefack, Télesphore Benoît; Ateufack, Gilbert; Watcho, Pierre; Ndontsa, Blanche Laure; Tané, Pierre

doi:10.2202/1553-3840.1255

Cited by 18 publications

(18 citation statements)

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“…However, standard ascorbic acid has shown strong antioxidant activity than test samples. e phytochemical studies reported showed that stem bark of Croton macrostachyus constituted major secondary metabolites such as flavonoids, phenols, terpenoids, saponins, tannins, alkaloids, and steroids [19,20]. ese phytochemicals were reported to have an antioxidant activity in previous studies [33][34][35][36][37][38][39][40].…”

Section: Discussionmentioning

confidence: 99%

“…e plant showed antibacterial [15], antidiarrheal [16], anticonvulsant [17], antidiabetic [14], and antihyperalgesic activity [18]. e phytochemical screening showed that the stem bark of Croton macrostachyus constituted the major secondary metabolites such as tannins, steroids, alkaloids, phenols, terpenoids, saponins, and flavonoids, which might be the reason for the plant widespread pharmacological activity [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Inflammation was associated with pronounced cardiac damage like heart failure [64]. e stem bark extract of Croton macrostachyus was reported to have an anti-inflammatory effect and this might be one possible reason for its protective effect [19]. Results are expressed as mean ± SEM (n � 6).…”

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confidence: 99%

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Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide‐Induced Cardiotoxicity in Rats

Ayza

Balasubramanian²,

Berhe

2020

Evidence-Based Complementary and Alternative Medicine

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Context. Croton macrostachyus Hochst. ex Delile (Euphorbiaceae) has been used in traditional medicine to manage heart failure and other heart diseases in Ethiopia. Objective. To evaluate the antioxidant and cardioprotective activities of stem bark extract and solvent fractions of Croton macrostachyus on cyclophosphamide-induced cardiotoxicity in rats. Materials and Methods. DPPH free radical scavenging assay method was used to determine antioxidant activity whereas Sprague-Dawley rats were used to evaluate the cardioprotective activity. Except for the normal control, all groups were subjected to cyclophosphamide (200 mg/kg, i.p.) toxicity on the first day. Enalapril at 10 mg/kg was used as a reference. The hydromethanolic crude extract (100, 200, and 400 mg/kg) and aqueous and ethyl acetate fractions (100 and 200 mg/kg, each) were administered for 10 days. The cardioprotective activities were evaluated using cardiac biomarkers such as Troponin I, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), and histopathological studies of heart tissue. Results. Crude extract and ethyl acetate and aqueous fractions exhibited free radical scavenging activities at IC50 of 594 μg/mL, 419 μg/mL, and 716 μg/mL, respectively. Crude extract at 400 mg/kg decreased the levels of troponin, AST, ALT, and ALP to 0.29 ± 0.06 ng/mL, 103.00 ± 7.63 U/L, 99.80 ± 6.18 U/L, and 108.80 ± 8.81 U/L, respectively. In addition, ethyl acetate fraction at 200 mg/kg decreased the levels of troponin, AST, ALT, and ALP to 0.22 ± 0.02 ng/mL, 137.00 ± 14.30 U/L, 90.33 ± 6.13 U/L, and 166.67 ± 13.50 U/L, respectively, compared with the cyclophosphamide control group. Conclusions. Croton macrostachyus possesses cardioprotective activities and it could be a possible source of treatment for cardiotoxicity induced by cyclophosphamide.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide‐Induced Cardiotoxicity in Rats

Ayza

Balasubramanian²,

Berhe

2020

Evidence-Based Complementary and Alternative Medicine

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“…Croton macrostachyus (Hochst) pipe cleaner ex and Galinier, is a plant used in African folk medicine to treat different ailments, including pain and inflammation [ 7 , 8 ]. Indeed, previous studies have shown that aqueous and organic extracts from the stem bark of C. macrostachyus given orally, possess antinociceptive and anti-inflammatory activities in distinct acute animal models [ 9 ]. The present study aimed at providing additional insight about the antinociceptive effects of the methanol/methylene chloride extract of the stem bark of C. macrostachyus in different models of chronic inflammatory and neuropathic pain and to evaluate its preliminary mechanisms of action.…”

Section: Introductionmentioning

confidence: 99%

TRPV1 channel inhibition contributes to the antinociceptive effects of Croton macrostachyus extract in mice

Nguelefack

Dutra

Paszcuk

et al. 2015

BMC Complement Altern Med

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BackgroundPrevious study showed that extracts from Croton macrostachyus (Euphorbiaceae) exhibit analgesic effects in acute pain models. The present study evaluates the antinociceptive properties of the methanol/methylene chloride extract (MECM) of the stem bark of this plant using mice models of persistent inflammatory and neuropathic pain, and assesses its mechanism of action.MethodsMECM was tested on Complete Freund adjuvant (CFA)-induced persistent thermal and mechanical pain, neuropathic pain induced by partial sciatic nerve ligation (PSNL), prostaglandin E2 (PGE2)-induced acute mechanical hyperalgesia, as well as on nociception induced by capsaicin in mice. Mechanical hyperalgesia was assessed using von Frey hair in awake mice. The mechanism of action of MECM was evaluated by using glibenclamide on PGE2-induced hyperalgesia or rimonabant on capsaicin-induced pain.ResultsMECM administered orally at the doses of 250 and 500 mg/kg, induced long lasting and significant antihyperalgesic effects on CFA-inflammatory and PSNL-induced neuropathic pain. MECM significantly reduced the mechanical hyperalgesia induced by PGE2 either when administered preventively or therapeutically. MECM also significantly and time dependently inhibited the capsaicin-induced nociception. These effects were not affected by glibenclamide or by rimonabant.ConclusionsThe present results demonstrate that the oral administration of MECM to mice resulted in long lasting antihyperalgesic activity in inflammatory and neuropathic pain as well as in acute and persistent pain. The mechanism underlying the long lasting MECM antihyperalgesic effect is currently unknown, but might be mediated, at least partially, through the modulation of TRPV1 receptors.

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“…The plant showed antimicrobial activity against pathogens which are common contaminants of wound, such as Staphylococcus aureus and Pseudomonas aeruoginosa (Kalayou et al, 2012;Taye et al, 2011). The plant is also reported to have significant anti-inflammatory and analgesic activities in animal models (Kamanyi et al, 2009). Even though majority of the Ethiopian population uses traditional medicine, only limited studies were conducted on the traditional medicinal plants compared to the multicultural diversity and the diverse flora of the country (Tekalign et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Experimental evaluation of wound healing activity of Croton macrostachyus in rat

Mechesso¹,

Tadese²,

Tesfaye³

et al. 2016

Afr. J. Pharm. Pharmacol.

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Croton macrostachyus leaves are used for treatment of wounds by traditional healers in Ethiopia. Despite the use of this plant in the treatment of wound, there is limited data to support its medicinal use. The present study provides scientific evaluation for the wound healing potential of methanolic extract of C. macrostachyus leaves in rats. The leaves of Croton macrostachyus were studied for the presence of some secondary metabolites and wound healing activity. Ointments were made by incorporating the methanolic extract in simple ointment base B.P. in the concentration of 5 and 10% (w/w). Standard Nitrofurazone was used for comparison. Wound healing activity was studied, using excision and incision wound models. In excision wound model, percentage wound contraction, period of epithelization and morphological changes on the healed wounds were studied while incision wound model was used to determine breaking strength. The results were expressed as mean ± standard error of mean (SEM) and comparisons among treatment groups were made using one-way analysis of variance. Phytochemical screening of the methanolic extracts of leaves of C. macrostachyus showed the presence of different metabolites such as flavonoids and saponins which are reported to have significant wound healing activity. The results of epithelization period, percentage of wound contraction and morphological evaluation of groups of animals in the test groups showed significant (p < 0.05) wound healing activity compared to those treated with simple ointment. Similarly, the difference in breaking strength was significant (p < 0.05) for both 5 and 10% (w/w) methanol extract of C. macrostachyus ointment treated groups. Morphological evaluation showed a relatively better healing and growth of hair around the wound area in the 10% methanol extract of C. macrostachyus ointment treated group. Methanolic extract of C. macrostachyus enhanced wound healing significantly, corroborating the folk medicinal use of this plant.

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Anti-Nociceptive and Anti-Inflammatory Activities of Extracts from the Stem Bark of Croton macrostachyus (Euphorbiaceae) in Mice and Rats

Cited by 18 publications

References 0 publications

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide‐Induced Cardiotoxicity in Rats

Cardioprotective Effect of Croton macrostachyus Stem Bark Extract and Solvent Fractions on Cyclophosphamide‐Induced Cardiotoxicity in Rats

TRPV1 channel inhibition contributes to the antinociceptive effects of Croton macrostachyus extract in mice

Experimental evaluation of wound healing activity of Croton macrostachyus in rat

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