Experimental evaluation of wound healing activity of Croton macrostachyus in rat

Abstract

Croton macrostachyus leaves are used for treatment of wounds by traditional healers in Ethiopia. Despite the use of this plant in the treatment of wound, there is limited data to support its medicinal use. The present study provides scientific evaluation for the wound healing potential of methanolic extract of C. macrostachyus leaves in rats. The leaves of Croton macrostachyus were studied for the presence of some secondary metabolites and wound healing activity. Ointments were made by incorporating the methanolic extract in simple ointment base B.P. in the concentration of 5 and 10% (w/w). Standard Nitrofurazone was used for comparison. Wound healing activity was studied, using excision and incision wound models. In excision wound model, percentage wound contraction, period of epithelization and morphological changes on the healed wounds were studied while incision wound model was used to determine breaking strength. The results were expressed as mean ± standard error of mean (SEM) and comparisons among treatment groups were made using one-way analysis of variance. Phytochemical screening of the methanolic extracts of leaves of C. macrostachyus showed the presence of different metabolites such as flavonoids and saponins which are reported to have significant wound healing activity. The results of epithelization period, percentage of wound contraction and morphological evaluation of groups of animals in the test groups showed significant (p < 0.05) wound healing activity compared to those treated with simple ointment. Similarly, the difference in breaking strength was significant (p < 0.05) for both 5 and 10% (w/w) methanol extract of C. macrostachyus ointment treated groups. Morphological evaluation showed a relatively better healing and growth of hair around the wound area in the 10% methanol extract of C. macrostachyus ointment treated group. Methanolic extract of C. macrostachyus enhanced wound healing significantly, corroborating the folk medicinal use of this plant.

Evaluation of Wound Healing Activity of Methanolic Crude Extract and Solvent Fractions of the Leaves of Vernonia auriculifera Hiern (Asteraceae) in Mice

“…1,[12][13][14]17 Many plants and various preparations thereof have been used traditionally concerning wound treatment, especially due to their immense potential to affect the wound healing process. 18 To mention some, Vernonia amygdalina Del, 19 Brucea antidysenterica J.F.Mill, 20 Achyranthes aspera, 21 Croton macrostachyus, 22 Datura stramonium, 23 Bersama abyssinica, 24 Rumexa byssinicus, 1 Cordial africana, 25 Aloe spp, 26 and many others.…”

In Vitro Antibacterial Effects of Chloroform, Methanol and Water Extracts of Croton Macrostachyus Stem Bark Against Escherichia Coli and Staphylococcus Aureus Standard and Clinical Strains

“…It is also characterized by creamy to yellow-white colored flowers with green (when young) to grey (at maturity) fruits. C. macrostachyus is commonly named as 'Bisana' in Amharic, Ethiopia and it is an important medicinal plant in East Africa including Ethiopia (Abraham et al, 2016). It is traditionally used for the treatment of wounds (Teklehaymanot and Giday, 2007;Giday et al, 2009;Abraham et al, 2016) malaria, rabies, and gonorrhea Tineaversi color, diarrhea, hepatitis, jaundice, and scabies (Teklehaymanot and Giday, 2007).…”

Evaluation of the Wound Healing Activity of Commelina Benghalensis Leaves Extract in Experimental Rats

“…The forceps on one side was hooked to a fixed rod of metal while the other forcep was joined to a thread suspended by weights running over a pulley. 7 As early as gapping of the wound occurred, addition of weights was ceased and simultaneously the weights were lifted so as to avoid entire wound opening. The weights required to produce gapping were noted.…”