Anti-Müllerian hormone profiling in prepubertal horses and its relationship with gonadal function

Scarlet, D.; Wulf, Manuela; Kuhl, Juliane; Köhne, Martin; Ille, Natascha; Conley, Alan J; Aurich, Christine

doi:10.1016/j.theriogenology.2018.05.012

Cited by 15 publications

(19 citation statements)

References 33 publications

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“…The second notable observation was the high degree of variation in AMH concentrations in filly foals at birth. This was not observed in a recent study involving a smaller number of filly foals that were included in a longitudinal study (Scarlet et al 2018). Several of the newborn fillies in the current study had AMH concentrations that were in a range expected of colts (Claes et al 2013).…”

Section: Discussioncontrasting

confidence: 75%

“…The analysis of samples from over 1100 females from birth through their third decade of life provides a unique view of how AMH concentrations change with age in this species. The results of a recent longitudinal study in horses indicated that AMH concentrations were fairly constant during the first 2 years of life and that concentrations at birth were correlated with those at 2 years of age (Scarlet et al 2018). As the current data illustrate, despite the gradual loss of ovarian follicles that occurs inevitably with age in mammals (Gougeon et al 1994, Faddy & Gosden 1995, 1996, Faddy 2000, Bristol-Gould et al 2006, Hansen et al 2008, Coxworth & Hawkes 2010, Monget et al 2012, it is only after 20 years of age that AMH concentrations begin to decline in mares, and thereafter to decline quite rapidly.…”

Section: Discussionsupporting

confidence: 49%

“…Given this variability, it is unclear what extremes of variation in follicle populations might exist on the ovaries of at least some foals at birth. If this variability truly exists in larger populations of neonatal fillies, even if significantly correlated with concentrations at 2 years of age as previously shown (Scarlet et al 2018), AMH at birth may not be very predictive of concentrations in later life. Regardless, it is clear that AMH concentrations actually increase as mares enter their most fertile years, and AMH only begins to reflect diminishing follicular reserves in mares as they approach their third decade of life.…”

Section: Discussionmentioning

confidence: 87%

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Anti-Müllerian hormone and ovarian aging in mares

Uliani

Conley

Corbin

et al. 2019

Journal of Endocrinology

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Anti-Müllerian hormone (AMH) is used as a marker of follicle population numbers and potential fertility in several species including horses but limited data exist across the lifespan. No one has decreased ovarian reserve experimentally to investigate whether a corresponding, quantitative decrease in AMH results. Concentrations of AMH across the lifespan were compiled from 1101 equine females sampled from birth to >33 years of age. Young and old mares (averaging 6 and 19 years) were hemi-ovariectomized and circulating AMH was assessed before and daily thereafter for 15 days. The remaining ovary was removed later and blood was drawn again before and after this second surgery for AMH determination. Polynomial regression analysis and analysis of mares grouped by 5-year intervals of age demonstrated AMH concentrations to be higher in mares aged 5–10 and 10–15 years than 0–5 years of age and lower in mares after 20 years of age. There was high variability in AMH concentrations among neonatal fillies, some of which had concentrations typical of males. Hemi-ovariectomy was followed by a decrease of AMH, almost exactly halving concentrations in intact mares. Concentrations of AMH had returned to intact levels in old mares before complete ovariectomy, as if exhibiting ovarian compensatory hypertrophy, but recovery of AMH was not evident in young mares. AMH may reflect ovarian senescence in mares after 20 years of age but is too variable to do so in the first two decades of life. The ovarian endocrine response to hemi-ovariectomy in mares appears to change with age.

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Section: Discussioncontrasting

confidence: 75%

Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 87%

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Anti-Müllerian hormone and ovarian aging in mares

Uliani

Conley

Corbin

et al. 2019

Journal of Endocrinology

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“…For instance, antral follicle count (AFC) serves as a marker for ovarian reserve (18). A positive correlation between AMH and AFC has been found in young (25), middle-aged and older mares (6). It was noted that AMH and AFC were highly repeatebale in mares (6).…”

Section: Resultsmentioning

confidence: 99%

Serum anti-Müllerian hormone levels during estrus and diestrus in mares

EVKURAN¹,

Kaşıkçı²

2020

Medycyna Weterynaryjna

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Anti-Müllerian hormone (AMH) is a homodimeric glycoprotin secreted by granulosa cells in postnatal females. AMH plays an important role in follicle recruitment. The aim of the study was to analyse AMH concentrations under the guidance of circulating estradiol and progesterone levels in mares. The study was conducted on 25 non-lactating mares with regular estrous cycles. Blood samples were collected during estrus and diestrus to analyse estradiol, progesterone and AMH concentrations. Estradiol and progesterone concentrations were significantly different (P < 0.001) between estrus and diestrus. AMH concentrations in estrus were significantly higher than those in diestrus (P < 0.05). According to the authors’ knowledge, this is the first report to suggest that the sexual cycle stage affects AMH concentrations in mares. Further investigations are needed to reveal factors affecting the AMH production and secretion pattern throughout the sexual cycle.

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“…This approach would result in economic benefits for stallion breeders and prevent legal disputes concerning warranty claims. Unfortunately, an approach to predict future fertility at a prepubertal age based on Anti-Müllerian-Hormone plasma concentration in colts failed (Scarlet et al 2018).…”

Section: Figmentioning

confidence: 99%

Semen quality evaluation in young stallions – feasibility and comparison of two different protocols

Köhne¹,

Brüning²,

Stuhtmann³

et al. 2020

PHK

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Semen analysis is a valuable approach to assess male fertility in the horse. The aim of this study was to evaluate the effects of two different semen collection protocols, stallion's age, season and previous rearing conditions on sperm parameters of young stallions. Semen samples were obtained from 23 stallions (age range: 30-42 months old) every six weeks. Stallions were reared under different conditions prior to the start of the study. Semen collection was either performed twice with an interval of one hour on a given day (protocol A) or once daily for seven days (protocol B). The ejaculates were examined for total sperm number (TSN), progressive motility (PM), plasma membrane integrity (PMI), DNA fragmentation index (DFI) and semen quality after storage at 5 °C for 24 hours. TSN and DFI were higher for protocol A as compared to protocol B. TSN in stallions submitted to protocol A (1 st ejaculate) was influenced by season, whereas TSN remained unchanged in stallions submitted to protocol B. PM exceeded 70 % in every ejaculate and the protocol did not significantly affect motility. PMI was lower in ejaculates of 33.4 months old stallions on average as compared to older individuals. No other semen parameter was affected by the stallions' age. In conclusion, breeding soundness examination in 33.4 months old stallions is feasible and semen analysis after daily collection for 7 days provides less variable results throughout the year than semen analysis with protocol A. Therefore, semen analysis after daily collection for 7 days is recommended for young stallions.

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Anti-Müllerian hormone profiling in prepubertal horses and its relationship with gonadal function

Cited by 15 publications

References 33 publications

Anti-Müllerian hormone and ovarian aging in mares

Anti-Müllerian hormone and ovarian aging in mares

Serum anti-Müllerian hormone levels during estrus and diestrus in mares

Semen quality evaluation in young stallions – feasibility and comparison of two different protocols

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