Anti-microbial Properties of Valeriana officinalis, Satureja bachtiarica and Thymus daenensis Methanolic Extracts against Helicobacter pylori

Abstract

Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori.
 Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used.
 Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The inhibition zone diameter was the highest at 10% concentration of methanolic extracts of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis and had the highest growth inhibitory effect at higher concentrations.
 Conclusion: The methanolic extracts of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis herbs showed high anti-H. pylori effects in high concentrations and among them, Satureja bachtiarica showed the highest antibacterial effect.

Antibacterial Effects of Thymus daenensis Celak Ethanolic Extract Against Food-borne Bacteria

“…(2017) [34] disclosed that the T. kotschyanus, T. vulgaris, and T. pubescens had the highest antibacterial effects amongst all examined thyme species. Antibacterial effects of diverse thymus species cultivated in Italy [38], Iran [39], Brazil [39], and Hungary [40] were reported in the literature. In keeping with this, further researches are required to assess the antibacterial effects of all types of the T. daenensis extract against diverse kinds of food-borne bacteria.…”

Antimicrobial properties of medicinal plants; The new therapeutic aspect of Valeriana officinalis

“…Valerenic acid can be used to in different disorders such as depression, chronic anxiety, and sleep disorders on the basis of previous studies [4]. Based on previous literatures, the valerenic acid component had antimicrobial effects on different microorganisms such as Helicobacter pylori, Staphylococcus aureus and Candida albicans and its methanolic extract functions were dose-dependent [6][7][8]. Since the antimicrobial activity of V. officinalis in the total extract was correlated with abundant monoterpenoids and sesquiterpenoids, more research on various aspects of pharmaceutical effects is suggested.…”