Anti-malarial activity and toxicity assessment of Himatanthus articulatus, a plant used to treat malaria in the Brazilian Amazon

Vale, Valdicley Vieira; Vilhena, Thyago da Costa; Trindade, Rafaela Cabral dos Santos da; Ferreira, Márlia Regina C.; Percário, Sandro; Soares, Luciana Ferreira; Pereira, Washington Luiz Assunção; Brandão, Geraldo Célio; Oliveira, Alaı́de Braga de; Dolabela, Maria Fâni; Vasconcelos, Flávio

doi:10.1186/s12936-015-0643-1

Cited by 22 publications

(10 citation statements)

References 43 publications

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“…The inactivity of H. articulatus extract and its fractions in promastigote may be related to the major iridoid plumeride . [12]. The plumeride contains five OH groups which makes very polar difficult to pass through the membrane parasite.…”

Section: Discussionmentioning

confidence: 99%

Anti-promastigote Activity of the Amazon Plants

Silva-Silva¹,

Monteiro²,

Brígido³

et al. 2017

JPP

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This study evaluated in vitro activity of ethanol extract, fractions, and isolated substance from Amazon species against promastigotes of L. amazonensis. The ethanol extracts were concentrated and fractionation. The anti-promastigote activity was evaluated through the cell viability assessment method (MTT). The ethanol extract, fractions, and isolated substance from Himatanthus articulatus and Parahancornia fasciculata were inactive in promastigote of L. amazonensis, as the ethanol extract of Physalis angulata. The hexane fractions from different parts of Montrichardia linifera showed anti-promastigote activity probably due to the presence of steroids and terpenes. All species in studies were inactive, except of M. linifera. The few polar constituents can be responsible for the activity. Therefore, the isolation and purification of the active on L. amazonensis promastigotes are urgently required.

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Section: Discussionmentioning

confidence: 99%

Anti-promastigote Activity of the Amazon Plants

Silva-Silva¹,

Monteiro²,

Brígido³

et al. 2017

JPP

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“…n an effort to find new strategies for malaria control, recent research has investigated the role medicinal plants in regulating infections with Plasmodium spp (Mohd Abd Razak et al 2014;Mubaraki et al 2014;Odediran et al 2014;Vale et al 2015)…”

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confidence: 99%

Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice

2015

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Malaria is still one of the most common infectious diseases and leads to various public health problems worldwide. Medicinal plants are promising sources for identifying novel agents with potential antimalarial activity. This study aimed to investigate the antimalarial and the antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice. Mice were divided into five groups. The first group served as a vehicle control; the second, third, fourth, and fifth groups were infected with 1 × 10(6) P. chabaudi-parasitized erythrocytes. Mice of the last three groups were gavaged with 100 μl of I. oblongifolia leave extract (IOLE) at a dose of 100, 200, and 300 mg IOLE/kg, respectively, once daily for 7 days. IOLE was significantly able to lower the percentage of parasitemia. The most effective dose was the 100 mg IOLE/kg, which could reduce the parasitemia from about 38 to 12 %. The infection induced spleen injury. This was evidenced by disorganization of spleen white and red pulps, appearance of hemozoin granules and parasitized erythrocytes. These changes in spleen led to the increased histological score. Also, the infection increased the spleen oxidative damage where the levels of nitrite/nitrate, malondialdehyde, and catalase were significantly altered. All these infection-induced parameters were significantly improved during IOLE treatment. In addition, the mRNA expression of inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha were upregulated after infection with P. chabaudi, whereas IOLE significantly reduced the expression of these genes. Our results indicate that I. oblongifolia leaves extract exhibits a significant antimalarial and antioxidant effects, and protects host spleen tissue from injuries induced by P. chabaudi.

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“…The ethanolic extract was subjected to extraction under reflux [ 49 ], yielding 4 fractions: hexane, dichloromethane, ethyl acetate, and methanol. The extract and its fractions were subjected to characterization in thin-layer chromatography (Silicycle, Quebec City, QC, Canada) (TLC; revelators: UV and Dragendorff’s reagent) and high-performance liquid chromatography with diode-array detection (HPLC-DAD, Manufacturer, Milford, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Flavopereirine—An Alkaloid Derived from Geissospermum vellosii—Presents Leishmanicidal Activity In Vitro

et al. 2019

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Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of Leishmania amazonensis. In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC50 of 0.23 and 0.15 μg/mL, respectively). The extract, fractions, and flavopereirine presented low toxicity, with high selectivity for the alkaloid. Furthermore, flavopereirine showed no violation of Lipinski’s rule of five, showing even better results than the known inhibitor of oligopeptidase B, antipain, with three violations. Flavopereirine also interacted with residue Tyr-499 of oligopeptidase B during the molecular dynamics simulations, giving a few insights of a possible favorable mechanism of interaction and a possible inhibitory pathway. Flavopereirine proved to be a promising molecule for its antileishmanial activity.

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Anti-malarial activity and toxicity assessment of Himatanthus articulatus, a plant used to treat malaria in the Brazilian Amazon

Cited by 22 publications

References 43 publications

Anti-promastigote Activity of the Amazon Plants

Anti-promastigote Activity of the Amazon Plants

Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice

Flavopereirine—An Alkaloid Derived from Geissospermum vellosii—Presents Leishmanicidal Activity In Vitro

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