Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions

Guo, Dawei; Zhu, Lifei; Huang, Zhihai; Zhou, Haixia; Ge, Yili; Ma, Wenjuan; Wu, Jie; Zhang, Xiuyan; Zhou, Xuefeng; Zhao, Yun; Gu, Ning

doi:10.1016/j.biomaterials.2013.07.015

Cited by 259 publications

(206 citation statements)

References 60 publications

Supporting

Mentioning

193

Contrasting

Unclassified

Order By: Relevance

“…AgNPs are known to promote ROS production and DNA damage (26,53,54) which is consistent with their potential use as cancer therapeutics (29,55,56). However, as with most cytotoxic drugs, there are invariably off-target effects which restrict their use and our data indicates that AgNPs induce syncytin-1 expression in leukaemic cell lines.…”

Section: Discussionsupporting

confidence: 80%

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Alqahtani

Promtong

Oliver

et al. 2016

MUTAGE

View full text Add to dashboard Cite

Human endogenous retrovirus (HERV) sequences make up approximately 8% of the human genome and increased expression of some HERV proteins has been observed in various pathologies including leukaemia and multiple sclerosis (MS). However, little is known about the function of these HERV proteins or environmental factors which regulate their expression. Silver nanoparticles (AgNPs) are used very extensively as antimicrobials and antivirals in numerous consumer products although their effect on the expression of HERV gene products is unknown. Cell proliferation and cell toxicity assays were carried out on human acute T lymphoblastic leukaemia (MOLT-4) and Fanconi anaemia associated acute myeloid leukaemia (FA-AML1) cells treated with two different sizes of AgNPs (7 nm and 50 nm diameter). RT-PCR and Western blotting were then used to the assess expression of HERV-W syncytin-1 mRNA and protein in these cells. FA-AML1 cells were more sensitive overall than MOLT-4 to treatment with the smaller 7 nm sized AgNp's being the most toxic in these cells. MOLT-4 cell were more resistant and showed no evidence of differential toxicity to the different sized particles. Syncytin-1 mRNA and protein were induced by both 7 and 50 nm AgNPs in both cell types yet with different kinetics. In summary, the observation that AgNPs induce expression of syncytin-1 in FA-AML1 and MOLT-4 cells at doses as little as 5 µg/ml is grounds for concern since this protein is up-regulated in both malignant and neurodegenerative diseases. Considering the widespread use of AgNPs in the environment it is clear that their ability to induce syncytin-1 should be investigated further in other cell types.3

show abstract

Section: Discussionsupporting

confidence: 80%

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Alqahtani

Promtong

Oliver

et al. 2016

MUTAGE

View full text Add to dashboard Cite

show abstract

“…Continued on next page 6 acute myeloid leukemia cell lines, showing IC 50 values ranging from 0.5-20 ppm of AgNps (Kim et al, 2009;Zhou and Wang, 2012;Guo et al, 2013). AuNPs inhibited the proliferation of multiple myeloma cell lines and human lung adenocarcinoma cells with IC 50 values ranging from 5-60 ppm (Bhattacharya et al, 2007;Choi et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Parnsamut¹,

Brimson²

2015

Genet. Mol. Res.

View full text Add to dashboard Cite

“…In this work, we also studied the synergic effect of Ali and AgNPs. AgNPs have already been proposed as anticancer agents because of their intrinsic toxic properties [34,35], and the AgNPs' cytotoxic effect against cancer cells has already been observed in vitro on U87MG cells, human leukemic K562 cells [36] and in vivo [37]. Regarding the stability of the radiolabeled NPs, DTPA and histidine challenges are the most efficient methods to determine stability.…”

Section: Discussionmentioning

confidence: 99%

Targeted Delivery of Silver Nanoparticles and Alisertib: In Vitro and In Vivo Synergistic Effect Against Glioblastoma

et al. 2014

View full text Add to dashboard Cite

Aim: Targeted biocompatible nanoplatforms presenting multiple therapeutic functions have great potential for the treatment of cancer. Materials & methods: Multifunctional nanocomposites formed by polymeric nanoparticles (PNPs) containing two cytotoxic agents -the drug alisertib and silver nanoparticles -were synthesized. These PNPs have been conjugated with a chlorotoxin, an active targeting 36-amino acid-long peptide that specifically binds to MMP-2, a receptor overexpressed by brain cancer cells. Results:The individual and synergistic activity of these two cytotoxic agents against glioblastoma multiforme was tested both in vitro and in vivo. The induced cytotoxicity in a human glioblastoma-astrocytoma epithelial-like cell line (U87MG) was studied in vitro through a trypan blue exclusion test after 48 and 72 h of exposure. Subsequently, the PNPs' biodistribution in healthy animals and their effect on tumor reduction in tumor-bearing mice were studied using PNPs radiolabeled with 99m Tc. Conclusion: Tumor reduction was achieved in vivo when using silver/alisertib@PNPs-chlorotoxin.

show abstract

Anti-leukemia activity of PVP-coated silver nanoparticles via generation of reactive oxygen species and release of silver ions

Cited by 259 publications

References 60 publications

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Targeted Delivery of Silver Nanoparticles and Alisertib: In Vitro and In Vivo Synergistic Effect Against Glioblastoma

Contact Info

Product

Resources

About