Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Parnsamut, C; Brimson, Sirikalaya

doi:10.4238/2015.april.17.15

Cited by 55 publications

(38 citation statements)

References 30 publications

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“…Herzog et al [47] reported no effect of Ag NPs on the cellular level of TNF-α gene expression similar to Ag NPs 1 in this experiment. Contrarily to the present findings, Parnsamut and Brimson [48] reported a TNF-α gene downregulation in T-lymphocyte leukemic (Jurkat) cells in response to Ag NPs, however, the dose they applied was below their calculated IC50. This variability in genes expression levels could illustrate the molecular basis for the lower IC50 of Ag NPs 2 compared to Ag NPs 1 despite the smaller size of the later.…”

Section: Discussionsupporting

confidence: 67%

In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes

Sayed

Sabry

Hedeab

et al. 2019

Egyptian Journal of Histology

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Introduction: In the field of medicine, silver nanoparticles (Ag NPs) with their distinctive characteristics represent an interesting new cancer therapy. Cancer is a serious disease, despite the recent advances in its treatment; there are still some cases that are resistant to the current therapy. Aim of the work: This study aimed at examining the characteristics of 2 forms of silver nanomaterial (Ag NPs 1 and Ag NPs 2) and investigating their effect on breast cancer (MCF7) and liver cancer (HEP-G2) as well as on human normal melanocytes cell lines (HBF4). Materials and methods: It was found that both samples of nanoparticles have negative surface charge and their mean sizes were 9.02 nm and 24.6 nm respectively. Both Ag NPs 1 and Ag NPs 2 had a dose-dependent cytotoxicity on MCF7 and HEP-G2 with negligible effect on normal cell line (HBF4) measured by MTT assay. Results: Inhibitory Concentration 50 of Ag NPs1 and Ag NPs2 were 20.06 and 16.03 nmol/ml respectively. The resultant cell death was via apoptosis as illustrated by the inverted light microscopy. RT-PCR assessment revealed significant upregulation in Caspase 3 gene expression in the malignant cell lines exposed to each silver nanomaterials and remarkable downregulation VEGF and upregulation in TNF-α in either cancer cell line exposed to Ag NPs2 only. Meanwhile, the change in these cellular markers was minimal in normal human melanocyte cell line. Conclusion: This study indicates that silver nanoparticles can be used as anticancer agent with minimal effect on normal tissues. The cell viability depends not only on the size of Ag NPs but also their shape, surface charge and degree of repulsion between particles and aggregation.

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Section: Discussionsupporting

confidence: 67%

In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes

Sayed

Sabry

Hedeab

et al. 2019

Egyptian Journal of Histology

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“…On the other hand, the use of nanoparticles is very promising, but this is less known and controllable, and it has been not used as a strategy of the present research in order to avoid potential cytotoxicity concerns [8]. A low dose of silver might have a beneficial effect on the bone formation, as it could promote the proliferation and osteogenesis of the mesenchymal stem cells and have an inhibition effect on the production of the Tumor Necrosis Factor cytokine (TNF-α), resulting in an inhibition of osteoclastogenesis and inflammation [9,10]; however, a wrong concentration can also lead to an opposite effect, resulting in strong cytotoxicity and imbalance toward the osteoclasts formation, thus interfering with the bone remodeling process [11].…”

Section: Introductionmentioning

confidence: 99%

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

et al. 2020

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Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this study is to develop antibacterial and bioactive surfaces (Ti6Al4V alloy and a silica-based bioactive glass) by chemical doping with strontium and/or silver ions. The surfaces were characterized by Scanning Electron Microscopy equipped with Energy Dispersive X ray Spectroscopy (SEM-EDS), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM). To better focus on the cells–bacteria competition for the implant surface, in addition to the standard assays for the evaluation of the bacteria adhesion (ISO22196) and for single-cell cultures or biofilm formation, an innovative set of co-cultures of cells and bacteria is here proposed to simulate a competitive surface colonization. The results suggest that all the bioactive tested materials were cytocompatible toward the bone progenitor cells representative for the self-healing process, and that the doped ones were effective in reducing the surface colonization from a pathogenic drug-resistant strain of Staphylococcus aureus. The co-cultures experiments demonstrated that the doped surfaces were able to protect the adhered osteoblasts from the bacteria colonization as well as prevent the infection prior to the surface colonization by the osteoblasts.

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“…Unlike the polymeric composite, the hybrid material presented a significant inhibition (60-70%; p < 0.05) of pro-inflammatory cytokines secretion (Figure 1). This effect was probably due to silver nanoparticles interference in distinct signaling pathways preventing cell proliferation, as discussed in previous studies [4].…”

mentioning

confidence: 58%

Anti-Inflammatory Activity of Biomaterials Intended for Periodontal Disease Treatment

Seciu

Crăciunescu

Stan

et al. 2019

Priorities of Chemistry for a Sustainable Development-Priochem

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Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Cited by 55 publications

References 30 publications

In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes

In Vitro Characterization and Evaluation of Silver Nanoparticles Cytotoxicity on Human “Liver and Breast” Cancer Cells Versus Normal Melanocytes

Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

Anti-Inflammatory Activity of Biomaterials Intended for Periodontal Disease Treatment

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