Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization

Abstract

Aim . To evaluate the anti-invasive effect of ethanol extracts of rhizome of Dryopteris crassirhizoma (EEDC) in matrix invasion and formation of functional invadopodia and to determine the anti-tumor effect of EEDC in a mouse model of mandibular invasion by gingival squamous cell carcinoma (SCC). Methods . The rhizome of D crassirhizoma was extracted in ethanol. The anti-invasive effect of EEDC was analyzed with a Matrigel-coated transwell invasion and 3D culture system. Crucial factors related to the control of cancer cell invasion by EEDC were determined using a human protease array. Molecular evidence supporting the anti-invasive effect of EEDC in oral SCC (OSCC) cells used an invadopodia-mediated extracellular matrix (ECM) degradation; an in vivo athymic mouse model was also provided. Results . EEDC treatment (10 µg/mL) suppressed transwell migration and invasion of HSC-3 OSCC cells without cytotoxicity. Decreased levels of matrix metalloprotease (MMP)-7, kalikrein 10, cathepsin V, MMP-2, and cathepsin D were also found in EEDC-treated HSC-3 cells based on human protease array. The anti-invasive effects of EEDC involved the suppression of invadopodia-mediated ECM degradation via inhibition of globular-actin elongation. The anti-invasive effect resulting from disturbance of functional invadopodia formation by EEDC was observed even at a low concentration of 5 µg/mL. The phosphorylation of cortactin involved in functional invadopodia formation was decreased at EEDC concentrations that inhibited invadopodia formation. The anti-tumor effect of EEDC was also observed in a mouse xenograft model. Administration of EEDC resulted in inhibition of tumor growth and progression. Conclusions . EEDC represents a potential anti-invasive and anti-tumor agent in cancer control.

NUDT1-Knockdown Inhibits Proliferation, Invasion and Migration of CAL27

“…NUDT1-knockdown also inhibits the invasion of gastric cancer and hepatocellular carcinoma [9,13], while has not been investigated in OSCC. The invasion ability of OSCC was regulated by invadopodia formation level [25]. During the invasion movement of OSCC, the cancer cells will generate the invadopodia rstly, which could secret the MMP2, MMP9 and MMP14 to break up the local and distance extracellular matrix respectively [26,27].…”

Anticoronaviral Activity of the Natural Phloroglucinols, Dryocrassin ABBA and Filixic Acid ABA from the Rhizome of Dryopteris crassirhizoma by Targeting the Main Protease of SARS-CoV-2

“…The rhizome of Dryopteris crassirhizoma Nakai. (Dryopteridaceae) has been used as traditional medicine in East Asia and was recently reported to have anticancer [12], antiinflammatory [13], antibacterial [14], and anthelmintic [15] activity as well as antiviral activity against dengue virus [16]. It was reported that from >20 phloroglucinols isolated from D. crassirhizoma, dryocrassin ABBA and filixic acid ABA exhibited inhibitory activity against the neuraminidase of influenza virus and inhibited influenza virus infection [17].…”

Dryopteris juxtapostia Root and Shoot: Determination of Phytochemicals; Antioxidant, Anti-Inflammatory, and Hepatoprotective Effects; and Toxicity Assessment

“…Dryopteris chrysocoma , Dryopteris blanfordii , and Dryopteris crassirhizoma were reported to exhibit anti-inflammatory activities [ 12 , 13 , 14 ]. In other studies, Dryopteris fragrans and Dryopteris crassirhizoma extracts were found to possess anticancer potential [ 15 , 16 ]. However, Dryopteris juxtapostia extracts have not been explored for biological potential to date.…”