Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study

Leite, Liz M.; Carvalho, Antônio Germano G.; Ferreira, Pollyanna L. F. Tavares; Pessoa, Igor Xavier; Gonçalves, Danilo Oliveira; Lopes, Amanda de Araújo; Góes, Jean Guilherme dos Santos; Castro-Alves, Victor Costa; Leal, Luzia Kalyne Almeida Moreira; Brito, Gerly A. C.; Viana, G. S. B.

doi:10.1007/s10787-011-0077-5

Cited by 97 publications

(59 citation statements)

References 49 publications

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“…Minocycline depresses the release of oxygen radicals from various types of cells, including leukocytes, and the production of nitric oxide, through its effect on nitric oxide synthase (Amin et al, 1996). Data from in vitro rat brain assays show that minocycline is an effective antioxidant, with radical scavenging potency, similarly to vitamin E (Kraus et al, 2005), confirming previous results from our laboratory (Leite et al, 2011).…”

Section: Discussionsupporting

confidence: 87%

“…Minocycline is known to present a variety of antiapoptotic and anti-inflammatory effects, and these actions are manifested at low doses of the drug, as previously observed by several models of inflammation in vivo (Leite et al, 2011).…”

Section: Discussionmentioning

confidence: 72%

“…Their results indicated that inhibiting microglial activity may be an important strategy in the treatment of diabetic retinopathy, and minocycline could be a good option for the the delay or prevention of vision loss associated with the disease. We recently showed that TNF-α and iNOS positive cells appear in much lower number in the paw and periodontium of minocycline-treated rats, in models of carrageenan-induced edema and periodontal disease (Leite et al, 2011;Menezes, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Minocycline decreases blood glucose and triglyceride levels and reverses histological and immunohisto-chemical alterations in pancreas, liver and kidney of alloxan-induced diabetic rats

Viana¹,

Pessoa²,

Ferreira³

et al. 2014

J. Diabetes Endocrinol.

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Increasing evidence suggests that oxidative stress and inflammation play major roles in diabetes mellitus and its complications. Furthermore, hyperglycemia increases the production of free radicals, resulting in oxidative stress. Minocycline presents potent anti-inflammatory and antioxidant activities, as evaluated by in vivo and in vitro models. In the present study, the minocycline anti-diabetic effect was assessed in the model of alloxan-induced diabetes. Alloxan was injected to male Wistar rats (50 mg/kg, intravenously), and their blood was collected 48 h later and also after treatments, for measurements of glycemia, triglycerides, cholesterol and liver transaminases. Groups of untreated diabetic controls and diabetic treated with minocycline (1 to 50 mg/kg, peritoneally, p.o.) or glibenclamide (5 mg/kg, p.o., as reference), for different periods, were used. Furthermore, slices of pancreas, liver and kidney were submitted to histological and immunohistochemical analyses. While significant decreases in glucose and triglycerides were shown at the 5th and mainly at the 30th days after minocycline treatments, as compared to the untreated diabetic group, no changes were observed in total cholesterol, alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels. Histological analyses of pancreas, liver and kidney showed that minocycline significantly reversed tissue alterations, as those seen in untreated diabetic animals. Besides, minocycline also reduced tumor necrosis factor (TNF)-alpha, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expressions. The beneficial minocycline effects in diabetes could be due, at least partly, to its antiinflammatory and antioxidant properties, indicating that this drug may be a therapeutic alternative in diabetes mellitus and other pathological conditions where inflammation plays a significant role.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

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Minocycline decreases blood glucose and triglyceride levels and reverses histological and immunohisto-chemical alterations in pancreas, liver and kidney of alloxan-induced diabetic rats

Viana¹,

Pessoa²,

Ferreira³

et al. 2014

J. Diabetes Endocrinol.

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“…Most conventional anti-inflammatory drugs including diclomol gel suppress inflammatory responses at the later phase of inflammation [23]. In this study, hexane fraction of C. afer balm formula suppressed xylene-induced ear thickness from 10 to 360 minutes which suggested that the anti-inflammatory activity of hexane fraction of C. afer balm formula could be through modulation of arachidonic acid metabolism.…”

Section: Discussionsupporting

confidence: 50%

Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model

Anyasor¹,

Ijituyi²

2018

A J Physiol Biochem Pharmacol

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Objective: This study was designed to evaluate the effects of topically applied hexane fraction and aqueous extract of Costus afer leaves balm and cream formula on xylene-induced ear edema. Methods: In vitro anti-inflammatory study was carried out using thermally induced protein denaturation and human erythrocyte membrane stabilization assays while in vivo anti-inflammatory study was performed using xylene-induced ear edema in rats. Results: Hexane fraction of C. afer leaves significantly inhibited thermally induced protein denaturation and stabilized human erythrocyte membrane against hypotonicity-induced hemolysis when compared with aqueous extract of C. afer leaves. Furthermore, in vivo study showed that 10% and 20% w/w hexane fraction of C. afer leaves balm formula at 360 minutes significantly suppressed xylene-induced ear edema by 82.02% (0.16 ± 0.01 mm) and 96.62% (0.03 ± 0.01 mm), respectively, when compared with 10% and 20% w/w aqueous extract of C. afer leaves cream formula with 55.06% (0.40 ± 0.01 mm) and 67.42% (0.29 ± 0.02 mm) inhibitions, respectively. Conclusion: The findings from this study revealed that hexane fraction of C. afer leaves balm formula exhibited higher anti-inflammatory activity against xylene-induced topical inflammation. ARTICLE HISTORY

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“…Previously, we demonstrated a potent anti-inflammatory effect of Mino acutely administered in several models of inflammation [70]. In addition, Mino sub-chronically injected significantly reversed neurochemical and behavior alterations, in models of global ischemia and PD in rats [71].…”

Section: Discussionmentioning

confidence: 99%

Minocycline exerts a neuroprotective action against 6-OHDA-induced neurotoxicity: in vivo and in vitro studies

Ferreira¹,

Viana²

2015

neurol neurosci

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Background: Parkinson's disease (PD) is a chronic neurological disorder characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta. The oxidative stress and inflammation, among other factors, are involved in the mechanisms of cell death in PD. We studied the neuroprotective properties of minocycline (Mino), focusing on its behavioral, neurochemical and immunohistochemical effects. Besides, Mino effects were also studied on an in vitro model of PD, as well as on the release of MPO in human neutrophils, and also its antioxidant activity.

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Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study

Abstract: Our data indicate that anti-inflammatory and antioxidant effects, involve the inhibition of iNOS and TNF-alpha, among other properties, and these encourage clinical studies of these compounds for new therapeutic applications, especially those were inflammation plays a role.

Cited by 97 publications

References 49 publications

Minocycline decreases blood glucose and triglyceride levels and reverses histological and immunohisto-chemical alterations in pancreas, liver and kidney of alloxan-induced diabetic rats

Minocycline decreases blood glucose and triglyceride levels and reverses histological and immunohisto-chemical alterations in pancreas, liver and kidney of alloxan-induced diabetic rats

Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model

Minocycline exerts a neuroprotective action against 6-OHDA-induced neurotoxicity: in vivo and in vitro studies

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