Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways

Abstract

BackgroundArctiin, isolated from Forsythia suspensa has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects in vitro. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages.MethodsWe investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes.ResultsArctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE2, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-κB in macrophages was inhibited by arctiin.ConclusionTaken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.

Natural lignans from Arctium lappa as antiaging agents in Caenorhabditis elegans

“…For example, arctigenin, one of the major bioactive lignans in A. lappa, has strong antiproliferative and apoptotic effects against different cancer cell lines (Ryu et al, 1995). Besides, arctigenin and arctiin have potent anti-inflammatory effects via inhibiting lipopolysaccharide-induced nitric oxide (NO) production and the release of pro-inflammatory cytokines in murine macrophages (Kou et al, 2011;Lee et al, 2011). However, a possible antiaging potential of the bioactive lignans from A. lappa has not been reported.…”

Arctigenin Attenuates Ischemia/Reperfusion Induced Ventricular Arrhythmias by Decreasing Oxidative Stress in Rats

“…As ischemia /reperfusion injury remains an under studied therapeutic target [4], there is growing interest in the evaluation of antioxidant phytochemicals capable of preventing or protecting stress of oxidative agents associated with myocardial ischemia/reperfusion injury. Arctigenin (ATG), a lignan extract from Fructus Arctii, is the dried fruit of Arctium Lappa L. It is known to possess anti -inflammatory, immunemodulatory, anti viral, anti microbial, anti carcinogenic, vasodilatory and antiplatelet aggregation properties [5][6][7][8]. The nuclear factor erythroid 2-related factor 2 (Nrf2) is induced in response to oxidative stress, which might be caused by increased levels of endogenously generated ROSs [9].…”

In vivo and In vitro of Arctiin Schistosomicidal Activity

“…Several biological activities have been described for Arctiin, a molecule isolated from the Arctium lappa L., such as antiinflammatory [16] and anti-proliferative [23][24][25][26]. In this work, we sought to investigate schistosomicidal potential of this molecule In vitro effects of arctiin at 50, 100 and 200 μM concentrations on parasite viability, motor activity and tegument damage evaluated on daily basis for 3 days [2,7].…”

“…Research of natural products has provided remarkable new drugs or drug leads to combat several diseases [14][15][16][17][18].…”

“…Arctiin, a dibenzylbutyrolactone lignan, is the main constituent found in fruits of A. lappa that displays several biological activities, especially anti-inflammatory ( Figure 1A) [15,16]. Thus, the aim of this study was to evaluate the in vitro and in vivo schistosomicidal effects of arctiin in mice infected with S. mansoni, which have not yet been described.…”